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Characterization of acetylated 4-O-methylglucuronoxylan isolated from aspen employing 1H and 13C NMR spectroscopy

Teleman, Anita; Lundqvist, Jon LU ; Tjerneld, Folke LU ; Stålbrand, Henrik LU and Dahlman, Olof (2000) In Carbohydrate Research 329(4). p.807-815
Abstract
Water-soluble hemicelluloses were extracted from milled aspen wood (Populus tremula) employing microwave oven treatment at 180oC for 10 min. The final pH of this extract was 3.5. From this extract oligo- and polysaccharides were isolated and subsequently fractionated by size-exclusion chromatography. The structures of the saccharides in three of the fractions obtained were determined by 1H and 13C NMR spectroscopy, using homonuclear and heteronuclear two-dimensional techniques. The polysaccharides present in the two fractions eluted first were O-acetyl-(4-O-methylglucurono)xylans. The average degree of acetylation of the xylose residues in these compounds was 0.6. The structural element... (More)
Water-soluble hemicelluloses were extracted from milled aspen wood (Populus tremula) employing microwave oven treatment at 180oC for 10 min. The final pH of this extract was 3.5. From this extract oligo- and polysaccharides were isolated and subsequently fractionated by size-exclusion chromatography. The structures of the saccharides in three of the fractions obtained were determined by 1H and 13C NMR spectroscopy, using homonuclear and heteronuclear two-dimensional techniques. The polysaccharides present in the two fractions eluted first were O-acetyl-(4-O-methylglucurono)xylans. The average degree of acetylation of the xylose residues in these compounds was 0.6. The structural element ->4)[4-O-Me-α-d-GlcpA-(1->2)][3-O-Ac]-β-d-Xylp-(1-> could also be identified. On the average, these two xylans were composed of the following (1->4)-linked β-d-xylopyranosyl structural elements: unsubstituted (50 mol%), 2-O-acetylated (13 mol%), 3-O-acetylated (21 mol%), 2,3-di-O-acetylated (6 mol%) and [MeGlcA α-(1->2)][3-O-acetylated] (10 mol%). Most of the 4-O-methylglucuronyl and acetyl substituents in the isolated polysaccharides survived the microwave oven treatment. The third fraction, eluted last, contained acetylated xylo-oligosaccharides, with minor contamination by an acetylated mannan. In the case of these xylo-oligosaccharides, the average degree of acetylation was 0.3. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Aspen wood (Populus tremula), Hemicellulose, Xylan, extraction, structure, O-Acetylation, fractionation, Microwave oven
in
Carbohydrate Research
volume
329
issue
4
pages
807 - 815
publisher
Elsevier
external identifiers
  • scopus:0034534373
ISSN
1873-426X
DOI
10.1016/S0008-6215(00)00249-4
language
English
LU publication?
yes
id
7e3230d7-aa93-41b5-ba95-22c2f18219a9 (old id 125204)
date added to LUP
2007-07-06 16:43:33
date last changed
2017-08-13 03:33:24
@article{7e3230d7-aa93-41b5-ba95-22c2f18219a9,
  abstract     = {Water-soluble hemicelluloses were extracted from milled aspen wood (Populus tremula) employing microwave oven treatment at 180oC for 10 min. The final pH of this extract was 3.5. From this extract oligo- and polysaccharides were isolated and subsequently fractionated by size-exclusion chromatography. The structures of the saccharides in three of the fractions obtained were determined by 1H and 13C NMR spectroscopy, using homonuclear and heteronuclear two-dimensional techniques. The polysaccharides present in the two fractions eluted first were O-acetyl-(4-O-methylglucurono)xylans. The average degree of acetylation of the xylose residues in these compounds was 0.6. The structural element ->4)[4-O-Me-α-d-GlcpA-(1->2)][3-O-Ac]-β-d-Xylp-(1-> could also be identified. On the average, these two xylans were composed of the following (1->4)-linked β-d-xylopyranosyl structural elements: unsubstituted (50 mol%), 2-O-acetylated (13 mol%), 3-O-acetylated (21 mol%), 2,3-di-O-acetylated (6 mol%) and [MeGlcA α-(1->2)][3-O-acetylated] (10 mol%). Most of the 4-O-methylglucuronyl and acetyl substituents in the isolated polysaccharides survived the microwave oven treatment. The third fraction, eluted last, contained acetylated xylo-oligosaccharides, with minor contamination by an acetylated mannan. In the case of these xylo-oligosaccharides, the average degree of acetylation was 0.3.},
  author       = {Teleman, Anita and Lundqvist, Jon and Tjerneld, Folke and Stålbrand, Henrik and Dahlman, Olof},
  issn         = {1873-426X},
  keyword      = {Aspen wood (Populus tremula),Hemicellulose,Xylan,extraction,structure,O-Acetylation,fractionation,Microwave oven},
  language     = {eng},
  number       = {4},
  pages        = {807--815},
  publisher    = {Elsevier},
  series       = {Carbohydrate Research},
  title        = {Characterization of acetylated 4-O-methylglucuronoxylan isolated from aspen employing 1H and 13C NMR spectroscopy},
  url          = {http://dx.doi.org/10.1016/S0008-6215(00)00249-4},
  volume       = {329},
  year         = {2000},
}