B-lineage commitment prior to surface expression of B220 and CD19 on hematopoietic progenitor cells
(2008) In Blood 112(4). p.1048-1055- Abstract
- Commitment of hematopoietic progenitor cells to B-lymphoid cell fate has been suggested to coincide with the development of PAX5-expressing B220(+)CD19(+) pro-B cells. We have used a transgenic reporter mouse, expressing human CD25 under the control of the B-lineage-restricted Igll1 (lambda 5) promoter to investigate the lineage potential of early progenitor cells in the bone marrow. This strategy allowed us to identify a reporter expressing LIN-B220(-)CD19(-)CD127(+)FLT3(+) SCA1(low)KIT(low) population that displays a lack of myeloid and a 90% reduction in in vitro T-cell potential compared with its reporter-negative counterpart. Gene expression analysis demonstrated that these lineage-restricted cells express B-lineage-associated genes... (More)
- Commitment of hematopoietic progenitor cells to B-lymphoid cell fate has been suggested to coincide with the development of PAX5-expressing B220(+)CD19(+) pro-B cells. We have used a transgenic reporter mouse, expressing human CD25 under the control of the B-lineage-restricted Igll1 (lambda 5) promoter to investigate the lineage potential of early progenitor cells in the bone marrow. This strategy allowed us to identify a reporter expressing LIN-B220(-)CD19(-)CD127(+)FLT3(+) SCA1(low)KIT(low) population that displays a lack of myeloid and a 90% reduction in in vitro T-cell potential compared with its reporter-negative counterpart. Gene expression analysis demonstrated that these lineage-restricted cells express B-lineage-associated genes to levels comparable with that observed in pro-B cells. These data suggest that B-lineage commitment can occur before the expression of B220 and CD19. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1252912
- author
- Månsson, Robert LU ; Zandi, Sasan ; Anderson, Kristina LU ; Martensson, Inga-Lill ; Jacobsen, Sten Eirik W LU ; Bryder, David LU and Sigvardsson, Mikael LU
- organization
- publishing date
- 2008
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Blood
- volume
- 112
- issue
- 4
- pages
- 1048 - 1055
- publisher
- American Society of Hematology
- external identifiers
-
- wos:000258392300026
- scopus:50849143861
- pmid:18495958
- ISSN
- 1528-0020
- DOI
- 10.1182/blood-2007-11-125385
- language
- English
- LU publication?
- yes
- id
- 4f074ea8-aec4-4c1f-9e78-33f2716ed684 (old id 1252912)
- date added to LUP
- 2016-04-01 12:24:21
- date last changed
- 2022-07-22 20:08:14
@article{4f074ea8-aec4-4c1f-9e78-33f2716ed684, abstract = {{Commitment of hematopoietic progenitor cells to B-lymphoid cell fate has been suggested to coincide with the development of PAX5-expressing B220(+)CD19(+) pro-B cells. We have used a transgenic reporter mouse, expressing human CD25 under the control of the B-lineage-restricted Igll1 (lambda 5) promoter to investigate the lineage potential of early progenitor cells in the bone marrow. This strategy allowed us to identify a reporter expressing LIN-B220(-)CD19(-)CD127(+)FLT3(+) SCA1(low)KIT(low) population that displays a lack of myeloid and a 90% reduction in in vitro T-cell potential compared with its reporter-negative counterpart. Gene expression analysis demonstrated that these lineage-restricted cells express B-lineage-associated genes to levels comparable with that observed in pro-B cells. These data suggest that B-lineage commitment can occur before the expression of B220 and CD19.}}, author = {{Månsson, Robert and Zandi, Sasan and Anderson, Kristina and Martensson, Inga-Lill and Jacobsen, Sten Eirik W and Bryder, David and Sigvardsson, Mikael}}, issn = {{1528-0020}}, language = {{eng}}, number = {{4}}, pages = {{1048--1055}}, publisher = {{American Society of Hematology}}, series = {{Blood}}, title = {{B-lineage commitment prior to surface expression of B220 and CD19 on hematopoietic progenitor cells}}, url = {{http://dx.doi.org/10.1182/blood-2007-11-125385}}, doi = {{10.1182/blood-2007-11-125385}}, volume = {{112}}, year = {{2008}}, }