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B-lineage commitment prior to surface expression of B220 and CD19 on hematopoietic progenitor cells

Månsson, Robert LU ; Zandi, Sasan; Anderson, Kristina LU ; Martensson, Inga-Lill; Jacobsen, Sten Eirik W LU ; Bryder, David LU and Sigvardsson, Mikael LU (2008) In Blood 112(4). p.1048-1055
Abstract
Commitment of hematopoietic progenitor cells to B-lymphoid cell fate has been suggested to coincide with the development of PAX5-expressing B220(+)CD19(+) pro-B cells. We have used a transgenic reporter mouse, expressing human CD25 under the control of the B-lineage-restricted Igll1 (lambda 5) promoter to investigate the lineage potential of early progenitor cells in the bone marrow. This strategy allowed us to identify a reporter expressing LIN-B220(-)CD19(-)CD127(+)FLT3(+) SCA1(low)KIT(low) population that displays a lack of myeloid and a 90% reduction in in vitro T-cell potential compared with its reporter-negative counterpart. Gene expression analysis demonstrated that these lineage-restricted cells express B-lineage-associated genes... (More)
Commitment of hematopoietic progenitor cells to B-lymphoid cell fate has been suggested to coincide with the development of PAX5-expressing B220(+)CD19(+) pro-B cells. We have used a transgenic reporter mouse, expressing human CD25 under the control of the B-lineage-restricted Igll1 (lambda 5) promoter to investigate the lineage potential of early progenitor cells in the bone marrow. This strategy allowed us to identify a reporter expressing LIN-B220(-)CD19(-)CD127(+)FLT3(+) SCA1(low)KIT(low) population that displays a lack of myeloid and a 90% reduction in in vitro T-cell potential compared with its reporter-negative counterpart. Gene expression analysis demonstrated that these lineage-restricted cells express B-lineage-associated genes to levels comparable with that observed in pro-B cells. These data suggest that B-lineage commitment can occur before the expression of B220 and CD19. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Blood
volume
112
issue
4
pages
1048 - 1055
publisher
American Society of Hematology
external identifiers
  • wos:000258392300026
  • scopus:50849143861
ISSN
1528-0020
DOI
10.1182/blood-2007-11-125385
language
English
LU publication?
yes
id
4f074ea8-aec4-4c1f-9e78-33f2716ed684 (old id 1252912)
date added to LUP
2008-10-31 08:52:26
date last changed
2017-07-02 03:43:30
@article{4f074ea8-aec4-4c1f-9e78-33f2716ed684,
  abstract     = {Commitment of hematopoietic progenitor cells to B-lymphoid cell fate has been suggested to coincide with the development of PAX5-expressing B220(+)CD19(+) pro-B cells. We have used a transgenic reporter mouse, expressing human CD25 under the control of the B-lineage-restricted Igll1 (lambda 5) promoter to investigate the lineage potential of early progenitor cells in the bone marrow. This strategy allowed us to identify a reporter expressing LIN-B220(-)CD19(-)CD127(+)FLT3(+) SCA1(low)KIT(low) population that displays a lack of myeloid and a 90% reduction in in vitro T-cell potential compared with its reporter-negative counterpart. Gene expression analysis demonstrated that these lineage-restricted cells express B-lineage-associated genes to levels comparable with that observed in pro-B cells. These data suggest that B-lineage commitment can occur before the expression of B220 and CD19.},
  author       = {Månsson, Robert and Zandi, Sasan and Anderson, Kristina and Martensson, Inga-Lill and Jacobsen, Sten Eirik W and Bryder, David and Sigvardsson, Mikael},
  issn         = {1528-0020},
  language     = {eng},
  number       = {4},
  pages        = {1048--1055},
  publisher    = {American Society of Hematology},
  series       = {Blood},
  title        = {B-lineage commitment prior to surface expression of B220 and CD19 on hematopoietic progenitor cells},
  url          = {http://dx.doi.org/10.1182/blood-2007-11-125385},
  volume       = {112},
  year         = {2008},
}