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Separation of amino acids and peptides by temperature induced phase partitioning. Theoretical model for partitioning and experimental data

Johansson, Hans-Olof LU ; Karlström, Gunnar LU and Tjerneld, Folke LU (1999) In Bioseparation 7(4-5). p.259-267
Abstract
There is a strong interest in use of `smart polymers' in separation systems. These are polymers which can react on external influence, such as temperature or pH change. With such polymers it is possible from the outside to affect the properties of a separation system. Amphiphilic copolymers show drastic changes in solubility properties, such as self-association and phase separation, at e.g. temperature increase. The random copolymers of ethylene oxide and propylene oxide units (EOPO-polymers) can form aqueous two-phase systems above the copolymer cloud point temperature. Two phases are formed, one consisting of 40-60% polymer in water and the other of almost 100% water. Amino acids and peptides can be partitioned in the thermoseparating... (More)
There is a strong interest in use of `smart polymers' in separation systems. These are polymers which can react on external influence, such as temperature or pH change. With such polymers it is possible from the outside to affect the properties of a separation system. Amphiphilic copolymers show drastic changes in solubility properties, such as self-association and phase separation, at e.g. temperature increase. The random copolymers of ethylene oxide and propylene oxide units (EOPO-polymers) can form aqueous two-phase systems above the copolymer cloud point temperature. Two phases are formed, one consisting of 40-60% polymer in water and the other of almost 100% water. Amino acids and peptides can be partitioned in the thermoseparating systems. The partitioning strongly depends on the solute hydrophobicity, where aromatic amino acids and peptides are partitioned to the polymer phase and hydrophilic to the water phase. Salt effects can be used to enhance the partitioning of charged molecules. The thermodynamic driving forces which govern the partitioning of molecules in a thermoseparated aqueous phase system is described with use of the Flory-Huggins theory for polymer solutions. Expressions are derived which show the entropic and enthalpic effects on solute partitioning. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
aqueous two-phase system, Flory-Huggins theory, peptides, phase separation, temperature-induced phase separation, thermoseparating polymer
in
Bioseparation
volume
7
issue
4-5
pages
259 - 267
publisher
Springer
ISSN
1573-8272
DOI
10.1023/A:1008063919674
language
English
LU publication?
yes
id
79d6077c-2f20-4365-9036-7c911c56cdba (old id 125336)
date added to LUP
2007-07-05 15:58:14
date last changed
2016-04-16 03:27:58
@article{79d6077c-2f20-4365-9036-7c911c56cdba,
  abstract     = {There is a strong interest in use of `smart polymers' in separation systems. These are polymers which can react on external influence, such as temperature or pH change. With such polymers it is possible from the outside to affect the properties of a separation system. Amphiphilic copolymers show drastic changes in solubility properties, such as self-association and phase separation, at e.g. temperature increase. The random copolymers of ethylene oxide and propylene oxide units (EOPO-polymers) can form aqueous two-phase systems above the copolymer cloud point temperature. Two phases are formed, one consisting of 40-60% polymer in water and the other of almost 100% water. Amino acids and peptides can be partitioned in the thermoseparating systems. The partitioning strongly depends on the solute hydrophobicity, where aromatic amino acids and peptides are partitioned to the polymer phase and hydrophilic to the water phase. Salt effects can be used to enhance the partitioning of charged molecules. The thermodynamic driving forces which govern the partitioning of molecules in a thermoseparated aqueous phase system is described with use of the Flory-Huggins theory for polymer solutions. Expressions are derived which show the entropic and enthalpic effects on solute partitioning.},
  author       = {Johansson, Hans-Olof and Karlström, Gunnar and Tjerneld, Folke},
  issn         = {1573-8272},
  keyword      = {aqueous two-phase system,Flory-Huggins theory,peptides,phase separation,temperature-induced phase separation,thermoseparating polymer},
  language     = {eng},
  number       = {4-5},
  pages        = {259--267},
  publisher    = {Springer},
  series       = {Bioseparation},
  title        = {Separation of amino acids and peptides by temperature induced phase partitioning. Theoretical model for partitioning and experimental data},
  url          = {http://dx.doi.org/10.1023/A:1008063919674},
  volume       = {7},
  year         = {1999},
}