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Novel polymer-polymer conjugates for recovery of lactic acid by aqueous two-phase extraction

Planas, Jordi; Kozlowski, Antoni; Harris, J Milton; Tjerneld, Folke LU and Hahn-Hägerdal, Bärbel LU (1999) In Biotechnology and Bioengineering 66(4). p.211-218
Abstract
A new family of polymer conjugates is proposed to overcome constraints in the applicability of aqueous two-phase systems for the recovery of lactic acid. Polyethylene glycol-polyethylenimine (PEI) conjugates and ethylene oxide propylene oxide-PEI (EOPO-PEI) conjugates were synthesized. Aqueous two-phase systems were generated when the conjugates were mixed with fractionated dextran or crude hydrolyzed starch. With 2% phosphate buffer in the systems, phase diagrams with critical points of 3.9% EOPO-PEI-3.8% dextran (DEX) and 3.5% EOPO-PEI-7.9% crude starch were obtained. The phase separation temperature of 10% EOPO-PEI solutions titrated with lactic acid to pH 6 was 35degreesC at 5% phosphate, and increased linearly to 63degreesC at 2%... (More)
A new family of polymer conjugates is proposed to overcome constraints in the applicability of aqueous two-phase systems for the recovery of lactic acid. Polyethylene glycol-polyethylenimine (PEI) conjugates and ethylene oxide propylene oxide-PEI (EOPO-PEI) conjugates were synthesized. Aqueous two-phase systems were generated when the conjugates were mixed with fractionated dextran or crude hydrolyzed starch. With 2% phosphate buffer in the systems, phase diagrams with critical points of 3.9% EOPO-PEI-3.8% dextran (DEX) and 3.5% EOPO-PEI-7.9% crude starch were obtained. The phase separation temperature of 10% EOPO-PEI solutions titrated with lactic acid to pH 6 was 35degreesC at 5% phosphate, and increased linearly to 63degreesC at 2% phosphate. Lactic acid partitioned to the top conjugate-rich phase of the new aqueous two-phase systems. In particular, the lactic acid partition coefficient was 2.1 in 10% EOPO-PEI-8% DEX systems containing 2% phosphate. In the same systems, the partitioning of the lactic acid bacterium, Lactococcus lactis subsp. lactis, was 0.45. The partitioning of propionic, succinic, and citric acids was also determined in the new aqueous two-phase systems. © 1999 John Wiley & Sons, Inc. Biotechnol Bioeng 66: 211-218, 1999. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
aqueous two-phase systems, lactic acid, partitioning, extraction, polyethylenimine
in
Biotechnology and Bioengineering
volume
66
issue
4
pages
211 - 218
publisher
John Wiley & Sons
external identifiers
  • scopus:0033396211
ISSN
1097-0290
DOI
10.1002/(SICI)1097-0290(1999)66:4<211::AID-BIT2>3.0.CO;2-1
language
English
LU publication?
yes
id
653fd123-f7e1-4300-b757-9b2c370882c7 (old id 125400)
date added to LUP
2007-06-28 16:59:47
date last changed
2017-01-01 04:33:29
@article{653fd123-f7e1-4300-b757-9b2c370882c7,
  abstract     = {A new family of polymer conjugates is proposed to overcome constraints in the applicability of aqueous two-phase systems for the recovery of lactic acid. Polyethylene glycol-polyethylenimine (PEI) conjugates and ethylene oxide propylene oxide-PEI (EOPO-PEI) conjugates were synthesized. Aqueous two-phase systems were generated when the conjugates were mixed with fractionated dextran or crude hydrolyzed starch. With 2% phosphate buffer in the systems, phase diagrams with critical points of 3.9% EOPO-PEI-3.8% dextran (DEX) and 3.5% EOPO-PEI-7.9% crude starch were obtained. The phase separation temperature of 10% EOPO-PEI solutions titrated with lactic acid to pH 6 was 35degreesC at 5% phosphate, and increased linearly to 63degreesC at 2% phosphate. Lactic acid partitioned to the top conjugate-rich phase of the new aqueous two-phase systems. In particular, the lactic acid partition coefficient was 2.1 in 10% EOPO-PEI-8% DEX systems containing 2% phosphate. In the same systems, the partitioning of the lactic acid bacterium, Lactococcus lactis subsp. lactis, was 0.45. The partitioning of propionic, succinic, and citric acids was also determined in the new aqueous two-phase systems. © 1999 John Wiley &amp; Sons, Inc. Biotechnol Bioeng 66: 211-218, 1999.},
  author       = {Planas, Jordi and Kozlowski, Antoni and Harris, J Milton and Tjerneld, Folke and Hahn-Hägerdal, Bärbel},
  issn         = {1097-0290},
  keyword      = {aqueous two-phase systems,lactic acid,partitioning,extraction,polyethylenimine},
  language     = {eng},
  number       = {4},
  pages        = {211--218},
  publisher    = {John Wiley & Sons},
  series       = {Biotechnology and Bioengineering},
  title        = {Novel polymer-polymer conjugates for recovery of lactic acid by aqueous two-phase extraction},
  url          = {http://dx.doi.org/10.1002/(SICI)1097-0290(1999)66:4<211::AID-BIT2>3.0.CO;2-1},
  volume       = {66},
  year         = {1999},
}