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Mannan-Degrading Enzymes from Cellulomonas fimi

Stoll, Dominik; Stålbrand, Henrik LU and Warren, R Antony (1999) In Applied and Environmental Microbiology 65(6). p.2598-2605
Abstract
The genes man26a and man2A from Cellulomonas fimi encode mannanase 26A (Man26A) and -mannosidase 2A (Man2A), respectively. Mature Man26A is a secreted, modular protein of 951 amino acids, comprising a catalytic module in family 26 of glycosyl hydrolases, an S-layer homology module, and two modules of unknown function. Exposure of Man26A produced by Escherichia coli to C. fimi protease generates active fragments of the enzyme that correspond to polypeptides with mannanase activity produced by C. fimi during growth on mannans, indicating that it may be the only mannanase produced by the organism. A significant fraction of the Man26A produced by C. fimi remains cell associated. Man2A is an intracellular enzyme comprising a catalytic module in... (More)
The genes man26a and man2A from Cellulomonas fimi encode mannanase 26A (Man26A) and -mannosidase 2A (Man2A), respectively. Mature Man26A is a secreted, modular protein of 951 amino acids, comprising a catalytic module in family 26 of glycosyl hydrolases, an S-layer homology module, and two modules of unknown function. Exposure of Man26A produced by Escherichia coli to C. fimi protease generates active fragments of the enzyme that correspond to polypeptides with mannanase activity produced by C. fimi during growth on mannans, indicating that it may be the only mannanase produced by the organism. A significant fraction of the Man26A produced by C. fimi remains cell associated. Man2A is an intracellular enzyme comprising a catalytic module in a subfamily of family 2 of the glycosyl hydrolases that at present contains only mammalian -mannosidases. (Less)
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organization
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type
Contribution to journal
publication status
published
subject
in
Applied and Environmental Microbiology
volume
65
issue
6
pages
2598 - 2605
publisher
American Society for Microbiology
external identifiers
  • scopus:0039180039
ISSN
0099-2240
language
English
LU publication?
yes
id
693e0c03-48a9-47fc-8534-7a72aeabffa4 (old id 125413)
alternative location
http://aem.asm.org/cgi/content/abstract/65/6/2598
date added to LUP
2007-07-06 15:46:54
date last changed
2017-04-23 03:34:00
@article{693e0c03-48a9-47fc-8534-7a72aeabffa4,
  abstract     = {The genes man26a and man2A from Cellulomonas fimi encode mannanase 26A (Man26A) and -mannosidase 2A (Man2A), respectively. Mature Man26A is a secreted, modular protein of 951 amino acids, comprising a catalytic module in family 26 of glycosyl hydrolases, an S-layer homology module, and two modules of unknown function. Exposure of Man26A produced by Escherichia coli to C. fimi protease generates active fragments of the enzyme that correspond to polypeptides with mannanase activity produced by C. fimi during growth on mannans, indicating that it may be the only mannanase produced by the organism. A significant fraction of the Man26A produced by C. fimi remains cell associated. Man2A is an intracellular enzyme comprising a catalytic module in a subfamily of family 2 of the glycosyl hydrolases that at present contains only mammalian -mannosidases.},
  author       = {Stoll, Dominik and Stålbrand, Henrik and Warren, R Antony},
  issn         = {0099-2240},
  language     = {eng},
  number       = {6},
  pages        = {2598--2605},
  publisher    = {American Society for Microbiology},
  series       = {Applied and Environmental Microbiology},
  title        = {Mannan-Degrading Enzymes from Cellulomonas fimi},
  volume       = {65},
  year         = {1999},
}