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Driving forces for phase separation and partitioning in aqueous two-phase systems

Johansson, Hans-Olof LU ; Karlström, Gunnar LU ; Tjerneld, Folke LU and Haynes, Charles A (1998) In Journal of Chromatography. B 711(1-2). p.3-17
Abstract
A set of simple analytical equations, derived from the Flory-Huggins theory, are used to identify the dominant driving forces for phase separation and solute (e.g., protein) partitioning, in the absence and presence of added electrolyte, in every general class of aqueous two-phase systems. The resulting model appears to capture the basic nature of two-phase systems and all trends observed experimentally. Case studies are used to identify fundamental differences in and the magnitudes of enthalpic and entropic contributions to partitioning in polymer-polymer (e.g., PEG-dextran), polymer-salt, and thermoseparating polymer-water (e.g., UCON-water) two-phase systems. The model therefore provides practitioners with a better understanding of... (More)
A set of simple analytical equations, derived from the Flory-Huggins theory, are used to identify the dominant driving forces for phase separation and solute (e.g., protein) partitioning, in the absence and presence of added electrolyte, in every general class of aqueous two-phase systems. The resulting model appears to capture the basic nature of two-phase systems and all trends observed experimentally. Case studies are used to identify fundamental differences in and the magnitudes of enthalpic and entropic contributions to partitioning in polymer-polymer (e.g., PEG-dextran), polymer-salt, and thermoseparating polymer-water (e.g., UCON-water) two-phase systems. The model therefore provides practitioners with a better understanding of partition systems, and industry with a simple, fundamental tool for selecting an appropriate two-phase system for a particular separation. (Less)
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author
; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Proteins
in
Journal of Chromatography. B
volume
711
issue
1-2
pages
3 - 17
publisher
Elsevier
external identifiers
  • scopus:0032569060
ISSN
1387-2273
DOI
10.1016/S0378-4347(97)00585-9
language
English
LU publication?
yes
additional info
The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Theoretical Chemistry (S) (011001039), Biochemistry and Structural Biology (S) (000006142)
id
7708fbae-0430-4464-84c0-d8a8465357d9 (old id 125552)
date added to LUP
2016-04-01 12:31:42
date last changed
2023-01-03 17:49:23
@article{7708fbae-0430-4464-84c0-d8a8465357d9,
  abstract     = {{A set of simple analytical equations, derived from the Flory-Huggins theory, are used to identify the dominant driving forces for phase separation and solute (e.g., protein) partitioning, in the absence and presence of added electrolyte, in every general class of aqueous two-phase systems. The resulting model appears to capture the basic nature of two-phase systems and all trends observed experimentally. Case studies are used to identify fundamental differences in and the magnitudes of enthalpic and entropic contributions to partitioning in polymer-polymer (e.g., PEG-dextran), polymer-salt, and thermoseparating polymer-water (e.g., UCON-water) two-phase systems. The model therefore provides practitioners with a better understanding of partition systems, and industry with a simple, fundamental tool for selecting an appropriate two-phase system for a particular separation.}},
  author       = {{Johansson, Hans-Olof and Karlström, Gunnar and Tjerneld, Folke and Haynes, Charles A}},
  issn         = {{1387-2273}},
  keywords     = {{Proteins}},
  language     = {{eng}},
  number       = {{1-2}},
  pages        = {{3--17}},
  publisher    = {{Elsevier}},
  series       = {{Journal of Chromatography. B}},
  title        = {{Driving forces for phase separation and partitioning in aqueous two-phase systems}},
  url          = {{http://dx.doi.org/10.1016/S0378-4347(97)00585-9}},
  doi          = {{10.1016/S0378-4347(97)00585-9}},
  volume       = {{711}},
  year         = {{1998}},
}