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Positively Regulated Glycerol/G3P-Dependent Bacillus subtilis Gene Expression System Based on Anti-Termination.

Lewin, Anna LU ; Su, Xiao-Dong and Hederstedt, Lars LU (2009) In Journal of Molecular Microbiology and Biotechnology 17. p.61-70
Abstract
Plasmid pLALA was constructed for glycerol or glycerol-3-phosphate inducible plasmid-borne gene expression in Bacillus subtilis and closely related Gram-positive bacteria. Gene expression using pLALA is based on anti-termination of transcription and involves the B. subtilis GlpP protein that in the presence of glycerol-3-phosphate acts as an anti-terminator protein by binding to the 5'-untranslated region of glpD mRNA. Properties and the usefulness of the system, denoted LALA, were validated by inducible production in B. subtilis strainsof two water-soluble proteins (beta-galactosidase and a protein phospho-tyrosine phosphatase), and one integral membrane protein (heme A synthase). Advantages with LALA is that it is based on positive... (More)
Plasmid pLALA was constructed for glycerol or glycerol-3-phosphate inducible plasmid-borne gene expression in Bacillus subtilis and closely related Gram-positive bacteria. Gene expression using pLALA is based on anti-termination of transcription and involves the B. subtilis GlpP protein that in the presence of glycerol-3-phosphate acts as an anti-terminator protein by binding to the 5'-untranslated region of glpD mRNA. Properties and the usefulness of the system, denoted LALA, were validated by inducible production in B. subtilis strainsof two water-soluble proteins (beta-galactosidase and a protein phospho-tyrosine phosphatase), and one integral membrane protein (heme A synthase). Advantages with LALA is that it is based on positive control, does not involve a DNA-binding protein, and that glycerol, a cheap and stable compound, can be used as inducer of gene expression. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Anti-termination, glp, Glycerol inducible, Bacillus subtilis
in
Journal of Molecular Microbiology and Biotechnology
volume
17
pages
61 - 70
publisher
Karger
external identifiers
  • wos:000268373100002
  • scopus:68149160868
ISSN
1464-1801
DOI
10.1159/000166210
language
English
LU publication?
yes
id
49b62a06-b757-4eec-8b8b-37961d8df1c1 (old id 1262036)
date added to LUP
2008-11-27 13:40:28
date last changed
2017-08-09 15:40:09
@article{49b62a06-b757-4eec-8b8b-37961d8df1c1,
  abstract     = {Plasmid pLALA was constructed for glycerol or glycerol-3-phosphate inducible plasmid-borne gene expression in Bacillus subtilis and closely related Gram-positive bacteria. Gene expression using pLALA is based on anti-termination of transcription and involves the B. subtilis GlpP protein that in the presence of glycerol-3-phosphate acts as an anti-terminator protein by binding to the 5'-untranslated region of glpD mRNA. Properties and the usefulness of the system, denoted LALA, were validated by inducible production in B. subtilis strainsof two water-soluble proteins (beta-galactosidase and a protein phospho-tyrosine phosphatase), and one integral membrane protein (heme A synthase). Advantages with LALA is that it is based on positive control, does not involve a DNA-binding protein, and that glycerol, a cheap and stable compound, can be used as inducer of gene expression.},
  author       = {Lewin, Anna and Su, Xiao-Dong and Hederstedt, Lars},
  issn         = {1464-1801},
  keyword      = {Anti-termination,glp,Glycerol inducible,Bacillus subtilis},
  language     = {eng},
  pages        = {61--70},
  publisher    = {Karger},
  series       = {Journal of Molecular Microbiology and Biotechnology},
  title        = {Positively Regulated Glycerol/G3P-Dependent <em>Bacillus subtilis</em> Gene Expression System Based on Anti-Termination.},
  url          = {http://dx.doi.org/10.1159/000166210},
  volume       = {17},
  year         = {2009},
}