A comparison of serum and EDTA plasma in the measurement of glutamic acid decarboxylase autoantibodies (GADA) and autoantibodies to islet antigen-2 (IA-2A) using the RSR radioimmunoassay (RIA) and enzyme linked immunosorbent assay (ELISA) kits.
Rahmati, Kobra ; Lernmark, Åke LU
; Becker, Charlotte
LU
; Foltyn Zadura, Anna
LU
; Larsson, Karin
; Ivarsson, Sten
LU
and Törn, Carina
LU
(2008)
In Clinical Laboratory
54(7-8).
p.227-235
- Abstract
- BACKGROUND: Glutamic acid decarboxylase antibodies (GADA) and tyrosine phosphatase antibodies (islet antigen-2 antibodies; IA-2A) are used in clinical practise to identify type 1 diabetes. METHODS: GADA and IA-2A were measured with RSR-ELISA kits in samples from 76 newly diagnosed type 1 diabetic children and 120 healthy controls. The aim was to evaluate performance of RSR-ELISA kits for GADA and IA-2A when serum and Ca2+ treated plasma were used. RESULTS: GADA achieved high area under the curve (AUC) both for serum 0.95 (95% CI 0.90-0.99) and for Ca2+ treated plasma 0.95 (95% CI 0.91-0.99). At specificity 98%, sensitivity was 84% for serum and 87% for Ca2+ treated plasma. IA-2A achieved AUC 0.92 (95% CI 0.87-0.97) for serum and 0.94 (95%... (More)
- BACKGROUND: Glutamic acid decarboxylase antibodies (GADA) and tyrosine phosphatase antibodies (islet antigen-2 antibodies; IA-2A) are used in clinical practise to identify type 1 diabetes. METHODS: GADA and IA-2A were measured with RSR-ELISA kits in samples from 76 newly diagnosed type 1 diabetic children and 120 healthy controls. The aim was to evaluate performance of RSR-ELISA kits for GADA and IA-2A when serum and Ca2+ treated plasma were used. RESULTS: GADA achieved high area under the curve (AUC) both for serum 0.95 (95% CI 0.90-0.99) and for Ca2+ treated plasma 0.95 (95% CI 0.91-0.99). At specificity 98%, sensitivity was 84% for serum and 87% for Ca2+ treated plasma. IA-2A achieved AUC 0.92 (95% CI 0.87-0.97) for serum and 0.94 (95% CI 0.90-0.98) for Ca2+ treated plasma. Using the lowest standard (15 WHO-Units/ml) as cut-off, specificity for serum was 100% and for Ca2+ treated plasma 99% with sensitivity 74% in both cases. Sensitivity was higher in ELISA compared to RIA (74%; p = 0.0080) for GADA measurement and similar for ELISA and RIA IA-2A measurements (76%; p = 0.50). CONCLUSION: Both RSR-ELISAs, GADA and IA-2A showed excellent performance for serum as well as for Ca2+ treated plasma. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1262060
- author
- Rahmati, Kobra
; Lernmark, Åke
LU
; Becker, Charlotte
LU
; Foltyn Zadura, Anna
LU
; Larsson, Karin
; Ivarsson, Sten
LU
and Törn, Carina
LU
- organization
- publishing date
- 2008
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Clinical Laboratory
- volume
- 54
- issue
- 7-8
- pages
- 227 - 235
- publisher
- Clin Lab. Publications
- external identifiers
-
- wos:000260167800001
- pmid:18942490
- scopus:55049133519
- ISSN
- 1433-6510
- language
- English
- LU publication?
- yes
- id
- 2e6d3430-8da2-4c54-be91-2935177daac3 (old id 1262060)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/18942490?dopt=Abstract
- date added to LUP
- 2016-04-04 09:37:25
- date last changed
- 2022-03-31 03:43:37
@article{2e6d3430-8da2-4c54-be91-2935177daac3,
abstract = {{BACKGROUND: Glutamic acid decarboxylase antibodies (GADA) and tyrosine phosphatase antibodies (islet antigen-2 antibodies; IA-2A) are used in clinical practise to identify type 1 diabetes. METHODS: GADA and IA-2A were measured with RSR-ELISA kits in samples from 76 newly diagnosed type 1 diabetic children and 120 healthy controls. The aim was to evaluate performance of RSR-ELISA kits for GADA and IA-2A when serum and Ca2+ treated plasma were used. RESULTS: GADA achieved high area under the curve (AUC) both for serum 0.95 (95% CI 0.90-0.99) and for Ca2+ treated plasma 0.95 (95% CI 0.91-0.99). At specificity 98%, sensitivity was 84% for serum and 87% for Ca2+ treated plasma. IA-2A achieved AUC 0.92 (95% CI 0.87-0.97) for serum and 0.94 (95% CI 0.90-0.98) for Ca2+ treated plasma. Using the lowest standard (15 WHO-Units/ml) as cut-off, specificity for serum was 100% and for Ca2+ treated plasma 99% with sensitivity 74% in both cases. Sensitivity was higher in ELISA compared to RIA (74%; p = 0.0080) for GADA measurement and similar for ELISA and RIA IA-2A measurements (76%; p = 0.50). CONCLUSION: Both RSR-ELISAs, GADA and IA-2A showed excellent performance for serum as well as for Ca2+ treated plasma.}},
author = {{Rahmati, Kobra and Lernmark, Åke and Becker, Charlotte and Foltyn Zadura, Anna and Larsson, Karin and Ivarsson, Sten and Törn, Carina}},
issn = {{1433-6510}},
language = {{eng}},
number = {{7-8}},
pages = {{227--235}},
publisher = {{Clin Lab. Publications}},
series = {{Clinical Laboratory}},
title = {{A comparison of serum and EDTA plasma in the measurement of glutamic acid decarboxylase autoantibodies (GADA) and autoantibodies to islet antigen-2 (IA-2A) using the RSR radioimmunoassay (RIA) and enzyme linked immunosorbent assay (ELISA) kits.}},
url = {{http://www.ncbi.nlm.nih.gov/pubmed/18942490?dopt=Abstract}},
volume = {{54}},
year = {{2008}},
}