Advanced

Analysis of phase composition in aqueous two-phase systems using a two-column chromatographic method: Application to lactic acid production by extractive fermentation

Planas, Jordi; Lefebvre, Denys; Tjerneld, Folke LU and Hahn-Hägerdal, Bärbel LU (1997) In Biotechnology and Bioengineering 54(4). p.303-311
Abstract
A new chromatographic system for the simultaneous analysis of polyethylene glycol, dextran, sugars, and low-molecular-weight fatty acids was developed. The system is based on a gel exclusion column which allows a first separation between high- and low-molecular-weight compounds, and a cationic exchange column used to further separate the low-molecular-weight compounds. Two applications of the system were demonstrated: (i) after optimizing eluent conditions the gel exclusion column was used to determine the influence of lactic acid, phosphate buffer, and lactic acid bacteria on the ethylene oxide propylene oxide-dextran T40 phase diagram by HPLC; (ii) the ion exchange column was coupled in series with the gel exclusion column and the... (More)
A new chromatographic system for the simultaneous analysis of polyethylene glycol, dextran, sugars, and low-molecular-weight fatty acids was developed. The system is based on a gel exclusion column which allows a first separation between high- and low-molecular-weight compounds, and a cationic exchange column used to further separate the low-molecular-weight compounds. Two applications of the system were demonstrated: (i) after optimizing eluent conditions the gel exclusion column was used to determine the influence of lactic acid, phosphate buffer, and lactic acid bacteria on the ethylene oxide propylene oxide-dextran T40 phase diagram by HPLC; (ii) the ion exchange column was coupled in series with the gel exclusion column and the concentration of polyethylene glycol, dextran, glucose, lactate, acetate, and formate was determined in samples from the fermentative production of lactic acid in a polyethylene glycol 8000-dextran T40 aqueous two-phase system. The fermentation was operated without pH control in a repeated extractive batch mode, where the cell-free top phase was replaced four times, whereas the cell-containing bottom phase was reused repeatedly. The yield was 1.1 mol of lactic acid formed per mole of glucose added and the productivity was 4.7 mM.h-1. The polymeric composition of the fermentation system was monitored during the five repeated extractive batches, and it showed a progressive depletion in polyethylene glycol and a progressive enrichment in dextran. © 1997 John Wiley & Sons, Inc. Biotechnol Bioeng 54: 303-311, 1997. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
lactic acid, ageous two-phase system, Lactococcus lactis, polymer analysis
in
Biotechnology and Bioengineering
volume
54
issue
4
pages
303 - 311
publisher
John Wiley & Sons
external identifiers
  • scopus:0343145697
ISSN
1097-0290
DOI
10.1002/(SICI)1097-0290(19970520)54:4<303::AID-BIT2>3.0.CO;2-O
language
English
LU publication?
yes
id
0db47200-5018-4477-8818-3593bc1129f9 (old id 126208)
date added to LUP
2007-06-28 16:46:09
date last changed
2017-07-23 03:51:17
@article{0db47200-5018-4477-8818-3593bc1129f9,
  abstract     = {A new chromatographic system for the simultaneous analysis of polyethylene glycol, dextran, sugars, and low-molecular-weight fatty acids was developed. The system is based on a gel exclusion column which allows a first separation between high- and low-molecular-weight compounds, and a cationic exchange column used to further separate the low-molecular-weight compounds. Two applications of the system were demonstrated: (i) after optimizing eluent conditions the gel exclusion column was used to determine the influence of lactic acid, phosphate buffer, and lactic acid bacteria on the ethylene oxide propylene oxide-dextran T40 phase diagram by HPLC; (ii) the ion exchange column was coupled in series with the gel exclusion column and the concentration of polyethylene glycol, dextran, glucose, lactate, acetate, and formate was determined in samples from the fermentative production of lactic acid in a polyethylene glycol 8000-dextran T40 aqueous two-phase system. The fermentation was operated without pH control in a repeated extractive batch mode, where the cell-free top phase was replaced four times, whereas the cell-containing bottom phase was reused repeatedly. The yield was 1.1 mol of lactic acid formed per mole of glucose added and the productivity was 4.7 mM.h-1. The polymeric composition of the fermentation system was monitored during the five repeated extractive batches, and it showed a progressive depletion in polyethylene glycol and a progressive enrichment in dextran. © 1997 John Wiley &amp; Sons, Inc. Biotechnol Bioeng 54: 303-311, 1997.},
  author       = {Planas, Jordi and Lefebvre, Denys and Tjerneld, Folke and Hahn-Hägerdal, Bärbel},
  issn         = {1097-0290},
  keyword      = {lactic acid,ageous two-phase system,Lactococcus lactis,polymer analysis},
  language     = {eng},
  number       = {4},
  pages        = {303--311},
  publisher    = {John Wiley & Sons},
  series       = {Biotechnology and Bioengineering},
  title        = {Analysis of phase composition in aqueous two-phase systems using a two-column chromatographic method: Application to lactic acid production by extractive fermentation},
  url          = {http://dx.doi.org/10.1002/(SICI)1097-0290(19970520)54:4<303::AID-BIT2>3.0.CO;2-O},
  volume       = {54},
  year         = {1997},
}