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Ucon-benzoyl dextran aqueous two-phase systems: protein purification with phase component recycling

Lu, Min; Albertsson, Per-Åke LU ; Johansson, Göte and Tjerneld, Folke LU (1996) In Journal of Chromatography. B 680(1-2). p.65-70
Abstract
Benzoyl dextran with a degree of substitution of 0.18 was synthesized by reacting dextran T500 with benzoyl chloride. A new type of aqueous two-phase system composed of benzoyl dextran as bottom phase polymer and the random copolymer of ethylene oxide and propylene oxide (Ucon 50-HB-5100) as top phase polymer has been formed. The phase diagram for the system Ucon 50-HB-5100-benzoyl dextran with a degree of substitution of 0.18 was determined at room temperature. This two-phase system has been used to purify 3-phosphoglycerate kinase from baker's yeast. The top-phase polymer (Ucon) can be separated from target enzyme by increasing the temperature. The bottom-phase polymer (benzoyl dextran) could be recovered by addition of salt. Yeast... (More)
Benzoyl dextran with a degree of substitution of 0.18 was synthesized by reacting dextran T500 with benzoyl chloride. A new type of aqueous two-phase system composed of benzoyl dextran as bottom phase polymer and the random copolymer of ethylene oxide and propylene oxide (Ucon 50-HB-5100) as top phase polymer has been formed. The phase diagram for the system Ucon 50-HB-5100-benzoyl dextran with a degree of substitution of 0.18 was determined at room temperature. This two-phase system has been used to purify 3-phosphoglycerate kinase from baker's yeast. The top-phase polymer (Ucon) can be separated from target enzyme by increasing the temperature. The bottom-phase polymer (benzoyl dextran) could be recovered by addition of salt. Yeast homogenate was partitioned in a primary Ucon 50-HB-5100-benzoyl dextran aqueous two-phase system. After phase separation the top phase was removed and temperature-induced phase separation was used for formation of a water phase and a Ucon-rich phase. The benzoyl dextran-enriched bottom phase from the primary system was diluted, and the polymer was separated from water by addition of Na2SO4. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Aqueous two-phase system, Partitioning, Ucon, Benzoyl dextran, Protein
in
Journal of Chromatography. B
volume
680
issue
1-2
pages
65 - 70
publisher
Elsevier
external identifiers
  • scopus:0029953740
ISSN
1873-376X
DOI
10.1016/0378-4347(95)00484-X
language
English
LU publication?
yes
id
1c0f4888-3997-4bde-a2bf-8187fbb4ac1b (old id 126314)
date added to LUP
2007-07-06 10:59:10
date last changed
2017-08-20 03:32:09
@article{1c0f4888-3997-4bde-a2bf-8187fbb4ac1b,
  abstract     = {Benzoyl dextran with a degree of substitution of 0.18 was synthesized by reacting dextran T500 with benzoyl chloride. A new type of aqueous two-phase system composed of benzoyl dextran as bottom phase polymer and the random copolymer of ethylene oxide and propylene oxide (Ucon 50-HB-5100) as top phase polymer has been formed. The phase diagram for the system Ucon 50-HB-5100-benzoyl dextran with a degree of substitution of 0.18 was determined at room temperature. This two-phase system has been used to purify 3-phosphoglycerate kinase from baker's yeast. The top-phase polymer (Ucon) can be separated from target enzyme by increasing the temperature. The bottom-phase polymer (benzoyl dextran) could be recovered by addition of salt. Yeast homogenate was partitioned in a primary Ucon 50-HB-5100-benzoyl dextran aqueous two-phase system. After phase separation the top phase was removed and temperature-induced phase separation was used for formation of a water phase and a Ucon-rich phase. The benzoyl dextran-enriched bottom phase from the primary system was diluted, and the polymer was separated from water by addition of Na2SO4.},
  author       = {Lu, Min and Albertsson, Per-Åke and Johansson, Göte and Tjerneld, Folke},
  issn         = {1873-376X},
  keyword      = {Aqueous two-phase system,Partitioning,Ucon,Benzoyl dextran,Protein},
  language     = {eng},
  number       = {1-2},
  pages        = {65--70},
  publisher    = {Elsevier},
  series       = {Journal of Chromatography. B},
  title        = {Ucon-benzoyl dextran aqueous two-phase systems: protein purification with phase component recycling},
  url          = {http://dx.doi.org/10.1016/0378-4347(95)00484-X},
  volume       = {680},
  year         = {1996},
}