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Protein partitioning in weakly charged polymer-surfactant aqueous two-phase systems

Sivars, Ulf; Bergfeldt, Karin; Piculell, Lennart LU and Tjerneld, Folke LU (1996) In Journal of Chromatography. B 680(1-2). p.43-53
Abstract
The study includes partitioning of proteins in aqueous two-phase systems consisting of the polymer dextran and the non-ionic surfactant C12E5 (pentaethylene glycol mono-n-dodecyl ether). In this system a micelle-enriched phase is in equilibrium with a polymer-enriched phase. Charges can be introduced into the micelles by the addition of charged surfactants. The charge of the mixed micelles is easily varied in sign and magnitude independently of pH, by the addition of different amounts of negatively charged surfactant, sodium dodecyl sulphate (SDS), or positively charged surfactant dodecyl trimethyl ammonium chloride (DoTAC). A series of water-soluble model proteins (BSA, β-lactoglobulin, myoglobin, cytochrome c and lysozyme), with... (More)
The study includes partitioning of proteins in aqueous two-phase systems consisting of the polymer dextran and the non-ionic surfactant C12E5 (pentaethylene glycol mono-n-dodecyl ether). In this system a micelle-enriched phase is in equilibrium with a polymer-enriched phase. Charges can be introduced into the micelles by the addition of charged surfactants. The charge of the mixed micelles is easily varied in sign and magnitude independently of pH, by the addition of different amounts of negatively charged surfactant, sodium dodecyl sulphate (SDS), or positively charged surfactant dodecyl trimethyl ammonium chloride (DoTAC). A series of water-soluble model proteins (BSA, β-lactoglobulin, myoglobin, cytochrome c and lysozyme), with different net charges at pH 7.1, have been partitioned in non-charged systems and in systems with charged mixed micelles or charged polymer (dextran sulphate). It is shown that partition coefficients for charged proteins in dextran-C12E5 systems can be strongly affected by addition of charged surfactants (SDS, DoTAC) or polymer (dextran sulphate) and that the effects are directly correlated to protein net charge. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Aqueous two-phase systems, Partitioning, Proteins, Surfactants, Dextrans
in
Journal of Chromatography. B
volume
680
issue
1-2
pages
43 - 53
publisher
Elsevier
external identifiers
  • scopus:0029898721
ISSN
1873-376X
DOI
10.1016/0378-4347(95)00564-1
language
English
LU publication?
yes
id
53988d0b-bdb5-4db8-8e3e-b06e50371818 (old id 126350)
date added to LUP
2007-07-06 15:22:22
date last changed
2017-01-01 04:47:09
@article{53988d0b-bdb5-4db8-8e3e-b06e50371818,
  abstract     = {The study includes partitioning of proteins in aqueous two-phase systems consisting of the polymer dextran and the non-ionic surfactant C12E5 (pentaethylene glycol mono-n-dodecyl ether). In this system a micelle-enriched phase is in equilibrium with a polymer-enriched phase. Charges can be introduced into the micelles by the addition of charged surfactants. The charge of the mixed micelles is easily varied in sign and magnitude independently of pH, by the addition of different amounts of negatively charged surfactant, sodium dodecyl sulphate (SDS), or positively charged surfactant dodecyl trimethyl ammonium chloride (DoTAC). A series of water-soluble model proteins (BSA, β-lactoglobulin, myoglobin, cytochrome c and lysozyme), with different net charges at pH 7.1, have been partitioned in non-charged systems and in systems with charged mixed micelles or charged polymer (dextran sulphate). It is shown that partition coefficients for charged proteins in dextran-C12E5 systems can be strongly affected by addition of charged surfactants (SDS, DoTAC) or polymer (dextran sulphate) and that the effects are directly correlated to protein net charge.},
  author       = {Sivars, Ulf and Bergfeldt, Karin and Piculell, Lennart and Tjerneld, Folke},
  issn         = {1873-376X},
  keyword      = {Aqueous two-phase systems,Partitioning,Proteins,Surfactants,Dextrans},
  language     = {eng},
  number       = {1-2},
  pages        = {43--53},
  publisher    = {Elsevier},
  series       = {Journal of Chromatography. B},
  title        = {Protein partitioning in weakly charged polymer-surfactant aqueous two-phase systems},
  url          = {http://dx.doi.org/10.1016/0378-4347(95)00564-1},
  volume       = {680},
  year         = {1996},
}