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On-line quantitation of enzymatic mannan hydrolysates in small-volume bioreactors by microdialysis sampling and column liquid chromatography-integrated pulsed electrochemical detection

Torto, Nelson; Marko-Varga, György LU ; Gorton, Lo LU ; Stålbrand, Henrik LU and Tjerneld, Folke LU (1996) In Journal of Chromatography A 725(1). p.165-175
Abstract
Quantitative aspects of on-line microdialysis sampling are investigated with respect to sampling in small-volume bioreactors. Three modes of microdialysis sampling; continuous-flow microdialysis sampling (CFMS), stopped-flow microdialysis sampling with continuous stirring, and stopped-flow microdialysis sampling with stopped stirring were investigated as a possible means for studying bioprocesses. The hydrolytic properties of a well characterised endomannanase from Aspergillus niger were studied using 0.01% ivory nut mannan as substrate in a 5-ml reaction vessel. On-line sampling was achieved using a microdialysis probe fitted with an SPS 6005 membrane. Stopped-flow microdialysis sampling was found to give the least analyte depletion and... (More)
Quantitative aspects of on-line microdialysis sampling are investigated with respect to sampling in small-volume bioreactors. Three modes of microdialysis sampling; continuous-flow microdialysis sampling (CFMS), stopped-flow microdialysis sampling with continuous stirring, and stopped-flow microdialysis sampling with stopped stirring were investigated as a possible means for studying bioprocesses. The hydrolytic properties of a well characterised endomannanase from Aspergillus niger were studied using 0.01% ivory nut mannan as substrate in a 5-ml reaction vessel. On-line sampling was achieved using a microdialysis probe fitted with an SPS 6005 membrane. Stopped-flow microdialysis sampling was found to give the least analyte depletion and thus used for quantitation of the enzymatic hydrolysates. However, CFMS can be used when analyte depletion is not significant (large-volume reactor). Hydrolysis of ivory nut mannan gave mainly mannobiose and mannotriose in almost equal amounts, which is consistent with an endo-wise hydrolysis. The concentrations of mannose and mannopentaose did not change significantly over the monitoring period, however, that of mannotetraose increased gradually up to 11 h where it starts to decrease. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Oligosaccharides, Mannoses, Mannan, Polysaccharides, β-Mannanase, Microdialysis, Sampling methods, Enzymes
in
Journal of Chromatography A
volume
725
issue
1
pages
165 - 175
publisher
Elsevier
external identifiers
  • scopus:0029924414
ISSN
0021-9673
DOI
10.1016/0021-9673(95)00945-0
language
English
LU publication?
yes
id
e550b606-1c4e-4379-8fc1-17f8718e5a1d (old id 126358)
date added to LUP
2007-06-28 09:14:18
date last changed
2017-03-05 04:25:49
@article{e550b606-1c4e-4379-8fc1-17f8718e5a1d,
  abstract     = {Quantitative aspects of on-line microdialysis sampling are investigated with respect to sampling in small-volume bioreactors. Three modes of microdialysis sampling; continuous-flow microdialysis sampling (CFMS), stopped-flow microdialysis sampling with continuous stirring, and stopped-flow microdialysis sampling with stopped stirring were investigated as a possible means for studying bioprocesses. The hydrolytic properties of a well characterised endomannanase from Aspergillus niger were studied using 0.01% ivory nut mannan as substrate in a 5-ml reaction vessel. On-line sampling was achieved using a microdialysis probe fitted with an SPS 6005 membrane. Stopped-flow microdialysis sampling was found to give the least analyte depletion and thus used for quantitation of the enzymatic hydrolysates. However, CFMS can be used when analyte depletion is not significant (large-volume reactor). Hydrolysis of ivory nut mannan gave mainly mannobiose and mannotriose in almost equal amounts, which is consistent with an endo-wise hydrolysis. The concentrations of mannose and mannopentaose did not change significantly over the monitoring period, however, that of mannotetraose increased gradually up to 11 h where it starts to decrease.},
  author       = {Torto, Nelson and Marko-Varga, György and Gorton, Lo and Stålbrand, Henrik and Tjerneld, Folke},
  issn         = {0021-9673},
  keyword      = {Oligosaccharides,Mannoses,Mannan,Polysaccharides,β-Mannanase,Microdialysis,Sampling methods,Enzymes},
  language     = {eng},
  number       = {1},
  pages        = {165--175},
  publisher    = {Elsevier},
  series       = {Journal of Chromatography A},
  title        = {On-line quantitation of enzymatic mannan hydrolysates in small-volume bioreactors by microdialysis sampling and column liquid chromatography-integrated pulsed electrochemical detection},
  url          = {http://dx.doi.org/10.1016/0021-9673(95)00945-0},
  volume       = {725},
  year         = {1996},
}