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Non-invasive, label-free cell counting and quantitative analysis of adherent cells using digital holography

Mölder, Anna; Sebesta, Mikael; Gustafsson, Mats LU ; Gisselson, Lennart; Gjörloff Wingren, Anette and Alm, Kersti LU (2008) In Journal of Microscopy 232(2). p.240-247
Abstract
Manual cell counting is time consuming and requires a high

degree of skill on behalf of the person performing the count.

Here we use a technique that utilizes digital holography,

allowing label-free and completely non-invasive cell counting

directly in cell culture vessels with adherent viable cells.

The images produced can provide both quantitative and

qualitative phase information from a single hologram.

The recently constructed microscope HolomonitorTM (Phase

Holographic Imaging AB, Lund, Sweden) combines the

commonly used phase contrast microscope with digital

holography, the latter giving us the possibility of achieving

quantitative... (More)
Manual cell counting is time consuming and requires a high

degree of skill on behalf of the person performing the count.

Here we use a technique that utilizes digital holography,

allowing label-free and completely non-invasive cell counting

directly in cell culture vessels with adherent viable cells.

The images produced can provide both quantitative and

qualitative phase information from a single hologram.

The recently constructed microscope HolomonitorTM (Phase

Holographic Imaging AB, Lund, Sweden) combines the

commonly used phase contrast microscope with digital

holography, the latter giving us the possibility of achieving

quantitative information on cellular shape, area, confluence

and optical thickness. This project aimed at determining the

accuracy and repeatability of cell counting measurements

using digital holography compared to the conventional

manual cell counting method using a haemocytometer. The

collected datawere also used to determine cell size and cellular

optical thickness.Theresults showthat digital holography can

be used for non-invasive automatic cell counting as precisely

as conventional manual cell counting (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Cell analysis, cellular optical thickness, cell size, cell counting, digital holography
in
Journal of Microscopy
volume
232
issue
2
pages
240 - 247
publisher
Wiley-Blackwell
external identifiers
  • wos:000260544500007
  • scopus:55449137792
ISSN
0022-2720
DOI
10.1111/j.1365-2818.2008.02095.x
language
English
LU publication?
yes
id
0b0c4c3b-0d2b-49ab-a214-94aa3c30c22f (old id 1270751)
date added to LUP
2008-11-27 13:05:50
date last changed
2017-10-01 03:40:49
@article{0b0c4c3b-0d2b-49ab-a214-94aa3c30c22f,
  abstract     = {Manual cell counting is time consuming and requires a high<br/><br>
degree of skill on behalf of the person performing the count.<br/><br>
Here we use a technique that utilizes digital holography,<br/><br>
allowing label-free and completely non-invasive cell counting<br/><br>
directly in cell culture vessels with adherent viable cells.<br/><br>
The images produced can provide both quantitative and<br/><br>
qualitative phase information from a single hologram.<br/><br>
The recently constructed microscope HolomonitorTM (Phase<br/><br>
Holographic Imaging AB, Lund, Sweden) combines the<br/><br>
commonly used phase contrast microscope with digital<br/><br>
holography, the latter giving us the possibility of achieving<br/><br>
quantitative information on cellular shape, area, confluence<br/><br>
and optical thickness. This project aimed at determining the<br/><br>
accuracy and repeatability of cell counting measurements<br/><br>
using digital holography compared to the conventional<br/><br>
manual cell counting method using a haemocytometer. The<br/><br>
collected datawere also used to determine cell size and cellular<br/><br>
optical thickness.Theresults showthat digital holography can<br/><br>
be used for non-invasive automatic cell counting as precisely<br/><br>
as conventional manual cell counting},
  author       = {Mölder, Anna and Sebesta, Mikael and Gustafsson, Mats and Gisselson, Lennart and Gjörloff Wingren, Anette and Alm, Kersti},
  issn         = {0022-2720},
  keyword      = {Cell analysis,cellular optical thickness,cell size,cell counting,digital holography},
  language     = {eng},
  number       = {2},
  pages        = {240--247},
  publisher    = {Wiley-Blackwell},
  series       = {Journal of Microscopy},
  title        = {Non-invasive, label-free cell counting and quantitative analysis of adherent cells using digital holography},
  url          = {http://dx.doi.org/10.1111/j.1365-2818.2008.02095.x},
  volume       = {232},
  year         = {2008},
}