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Incretin and islet hormonal responses to fat and protein ingestion in healthy men

Carr, Richard D.; Larsen, Marianne O.; Sörhede Winzell, Maria LU ; Jelic, Katarina; Lindgren, Ola LU ; Deacon, Carolyn F. and Ahrén, Bo LU (2008) In American Journal of Physiology: Endocrinology and Metabolism 295(4). p.779-784
Abstract
Glucagon-like peptide-1 (GLP-1) and glucose-dependent insulinotropic polypeptide (GIP) regulate islet function after carbohydrate ingestion. Whether incretin hormones are of importance for islet function after ingestion of noncarbohydrate macronutrients is not known. This study therefore examined integrated incretin and islet hormone responses to ingestion of pure fat (oleic acid; 0.88 g/kg) or protein (milk and egg protein; 2 g/kg) over 5 h in healthy men, aged 20-25 yr (n = 12); plain water ingestion served as control. Both intact (active) and total GLP-1 and GIP levels were determined as was plasma activity of dipeptidyl peptidase-4 (DPP-4). Following water ingestion, glucose, insulin, glucagon, GLP-1, and GIP levels and DPP-4 activity... (More)
Glucagon-like peptide-1 (GLP-1) and glucose-dependent insulinotropic polypeptide (GIP) regulate islet function after carbohydrate ingestion. Whether incretin hormones are of importance for islet function after ingestion of noncarbohydrate macronutrients is not known. This study therefore examined integrated incretin and islet hormone responses to ingestion of pure fat (oleic acid; 0.88 g/kg) or protein (milk and egg protein; 2 g/kg) over 5 h in healthy men, aged 20-25 yr (n = 12); plain water ingestion served as control. Both intact (active) and total GLP-1 and GIP levels were determined as was plasma activity of dipeptidyl peptidase-4 (DPP-4). Following water ingestion, glucose, insulin, glucagon, GLP-1, and GIP levels and DPP-4 activity were stable during the 5-h study period. Both fat and protein ingestion increased insulin, glucagon, GIP, and GLP-1 levels without affecting glucose levels or DPP-4 activity. The GLP-1 responses were similar after protein and fat, whereas the early (30 min) GIP response was higher after protein than after fat ingestion (P < 0.001). This was associated with sevenfold higher insulin and glucagon responses compared with fat ingestion (both P < 0.001). After protein, the early GIP, but not GLP-1, responses correlated to insulin (r(2) = 0.86; P = 0.0001) but not glucagon responses. In contrast, after fat ingestion, GLP-1 and GIP did not correlate to islet hormones. We conclude that, whereas protein and fat release both incretin and islet hormones, the early GIP secretion after protein ingestion may be of primary importance to islet hormone secretion. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
incretins, glucagon-like peptide-1, man, glucose-dependent, insulinotropic polypeptide, insulin, glucagon
in
American Journal of Physiology: Endocrinology and Metabolism
volume
295
issue
4
pages
779 - 784
publisher
American Physiological Society
external identifiers
  • wos:000259967600006
  • scopus:56049105157
ISSN
1522-1555
DOI
10.1152/ajpendo.90233.2008
language
English
LU publication?
yes
id
2c49784c-d53a-4b76-a92e-e2d3738f1439 (old id 1286136)
date added to LUP
2009-02-04 08:58:44
date last changed
2017-11-19 03:54:06
@article{2c49784c-d53a-4b76-a92e-e2d3738f1439,
  abstract     = {Glucagon-like peptide-1 (GLP-1) and glucose-dependent insulinotropic polypeptide (GIP) regulate islet function after carbohydrate ingestion. Whether incretin hormones are of importance for islet function after ingestion of noncarbohydrate macronutrients is not known. This study therefore examined integrated incretin and islet hormone responses to ingestion of pure fat (oleic acid; 0.88 g/kg) or protein (milk and egg protein; 2 g/kg) over 5 h in healthy men, aged 20-25 yr (n = 12); plain water ingestion served as control. Both intact (active) and total GLP-1 and GIP levels were determined as was plasma activity of dipeptidyl peptidase-4 (DPP-4). Following water ingestion, glucose, insulin, glucagon, GLP-1, and GIP levels and DPP-4 activity were stable during the 5-h study period. Both fat and protein ingestion increased insulin, glucagon, GIP, and GLP-1 levels without affecting glucose levels or DPP-4 activity. The GLP-1 responses were similar after protein and fat, whereas the early (30 min) GIP response was higher after protein than after fat ingestion (P &lt; 0.001). This was associated with sevenfold higher insulin and glucagon responses compared with fat ingestion (both P &lt; 0.001). After protein, the early GIP, but not GLP-1, responses correlated to insulin (r(2) = 0.86; P = 0.0001) but not glucagon responses. In contrast, after fat ingestion, GLP-1 and GIP did not correlate to islet hormones. We conclude that, whereas protein and fat release both incretin and islet hormones, the early GIP secretion after protein ingestion may be of primary importance to islet hormone secretion.},
  author       = {Carr, Richard D. and Larsen, Marianne O. and Sörhede Winzell, Maria and Jelic, Katarina and Lindgren, Ola and Deacon, Carolyn F. and Ahrén, Bo},
  issn         = {1522-1555},
  keyword      = {incretins,glucagon-like peptide-1,man,glucose-dependent,insulinotropic polypeptide,insulin,glucagon},
  language     = {eng},
  number       = {4},
  pages        = {779--784},
  publisher    = {American Physiological Society},
  series       = {American Journal of Physiology: Endocrinology and Metabolism},
  title        = {Incretin and islet hormonal responses to fat and protein ingestion in healthy men},
  url          = {http://dx.doi.org/10.1152/ajpendo.90233.2008},
  volume       = {295},
  year         = {2008},
}