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CRIM1 is localized to the podocyte filtration slit diaphragm of the adult human kidney

Nyström, Jenny; Hultenby, Kjell; Ek, Sara LU ; Sjölund, Jonas LU ; Axelson, Håkan LU ; Jirström, Karin LU ; Saleem, Moin; Nilsson, Kristina LU and Johansson, Martin LU (2009) In Nephrology Dialysis Transplantation 24(7). p.2038-2044
Abstract
Background. CRIM1 is a plasma membrane bound protein

containing six cysteine-rich repeats (CRR). Through these,

CRIM1 has been shown to interact with a subgroup of the

TGF-β superfamily, the bone morphogenic proteins (BMP)

isoforms 2, 4 and 7. The probable action is to modulate

the signalling properties of these factors. CRIM1 has also

been shown to regulate the release of VEGFA by podocytes

during renal organogenesis. Knock-out studies in mice have

shown that CRIM1 is critically involved in the development

of the central nervous system, eye and kidney. Replacement

of CRIM1 with a defective version leads to renal dysgenesis

and perinatal... (More)
Background. CRIM1 is a plasma membrane bound protein

containing six cysteine-rich repeats (CRR). Through these,

CRIM1 has been shown to interact with a subgroup of the

TGF-β superfamily, the bone morphogenic proteins (BMP)

isoforms 2, 4 and 7. The probable action is to modulate

the signalling properties of these factors. CRIM1 has also

been shown to regulate the release of VEGFA by podocytes

during renal organogenesis. Knock-out studies in mice have

shown that CRIM1 is critically involved in the development

of the central nervous system, eye and kidney. Replacement

of CRIM1 with a defective version leads to renal dysgenesis

and perinatal death. We have analysed the distribution of

CRIM1 in adult human renal tissue.

Methods. To this end, we have used immunofluorescence,

immunohistochemistry and immunoelectron microscopy.

We performed western blotting for the CRIM1 protein,

using lysates from isolated glomerular podocytes and human

renal tissue homogenate. By using quantitative PCR,

we compared the CRIM1 mRNA levels in podocytes, human

renal tissue homogenate, primary human renal proximal

tubular epithelial cells and primary human pulmonary

artery smooth muscle cells.

Results. The results show that in the human adult kidney,

CRIM1 is mainly expressed in the glomerular podocytes

and is associated with the insertional region of the filtration

slit diaphragm (SD) of the podocyte pedicles.

Conclusions. CRIM1 is a protein that should be added to

the list of proteins associated with the podocyte filtration

SD and with the probable action of modulating BMP and

VEGFA signalling. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
podocyte, immunoelectron microscopy, bone morphogenic protein, filtration slit membrane
in
Nephrology Dialysis Transplantation
volume
24
issue
7
pages
2038 - 2044
publisher
Oxford University Press
external identifiers
  • wos:000267226500009
  • pmid:19158190
  • scopus:67651121922
ISSN
1460-2385
DOI
10.1093/ndt/gfn743
language
English
LU publication?
yes
id
2b2194b6-312f-418f-adcc-fd1a9d95beda (old id 1288636)
date added to LUP
2009-01-30 14:43:56
date last changed
2017-01-01 06:35:48
@article{2b2194b6-312f-418f-adcc-fd1a9d95beda,
  abstract     = {Background. CRIM1 is a plasma membrane bound protein<br/><br>
containing six cysteine-rich repeats (CRR). Through these,<br/><br>
CRIM1 has been shown to interact with a subgroup of the<br/><br>
TGF-β superfamily, the bone morphogenic proteins (BMP)<br/><br>
isoforms 2, 4 and 7. The probable action is to modulate<br/><br>
the signalling properties of these factors. CRIM1 has also<br/><br>
been shown to regulate the release of VEGFA by podocytes<br/><br>
during renal organogenesis. Knock-out studies in mice have<br/><br>
shown that CRIM1 is critically involved in the development<br/><br>
of the central nervous system, eye and kidney. Replacement<br/><br>
of CRIM1 with a defective version leads to renal dysgenesis<br/><br>
and perinatal death. We have analysed the distribution of<br/><br>
CRIM1 in adult human renal tissue.<br/><br>
Methods. To this end, we have used immunofluorescence,<br/><br>
immunohistochemistry and immunoelectron microscopy.<br/><br>
We performed western blotting for the CRIM1 protein,<br/><br>
using lysates from isolated glomerular podocytes and human<br/><br>
renal tissue homogenate. By using quantitative PCR,<br/><br>
we compared the CRIM1 mRNA levels in podocytes, human<br/><br>
renal tissue homogenate, primary human renal proximal<br/><br>
tubular epithelial cells and primary human pulmonary<br/><br>
artery smooth muscle cells.<br/><br>
Results. The results show that in the human adult kidney,<br/><br>
CRIM1 is mainly expressed in the glomerular podocytes<br/><br>
and is associated with the insertional region of the filtration<br/><br>
slit diaphragm (SD) of the podocyte pedicles.<br/><br>
Conclusions. CRIM1 is a protein that should be added to<br/><br>
the list of proteins associated with the podocyte filtration<br/><br>
SD and with the probable action of modulating BMP and<br/><br>
VEGFA signalling.},
  author       = {Nyström, Jenny and Hultenby, Kjell and Ek, Sara and Sjölund, Jonas and Axelson, Håkan and Jirström, Karin and Saleem, Moin and Nilsson, Kristina and Johansson, Martin},
  issn         = {1460-2385},
  keyword      = {podocyte,immunoelectron microscopy,bone morphogenic protein,filtration slit membrane},
  language     = {eng},
  number       = {7},
  pages        = {2038--2044},
  publisher    = {Oxford University Press},
  series       = {Nephrology Dialysis Transplantation},
  title        = {CRIM1 is localized to the podocyte filtration slit diaphragm of the adult human kidney},
  url          = {http://dx.doi.org/10.1093/ndt/gfn743},
  volume       = {24},
  year         = {2009},
}