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SufA - a bacterial enzyme that cleaves fibrinogen and blocks fibrin network formation.

Karlsson, Christofer LU ; Mörgelin, Matthias LU ; Collin, Mattias LU ; Lood, Rolf LU ; Andersson, Marie-Louise LU ; Schmidtchen, Artur LU ; Björck, Lars LU and Frick, Inga-Maria LU (2009) In Microbiology1994-01-01+01:00 155(Pt 1). p.238-248
Abstract
Finegoldia magna is a member of the normal human bacterial flora on the skin and other non-sterile body surfaces, but this anaerobic coccus is also an important opportunistic pathogen. SufA was the first F. magna proteinase to be isolated and characterized. Many bacterial pathogens interfere with different steps of blood coagulation, and here we describe how purified SufA efficiently and specifically cleaves fibrinogen in human plasma. SufA is both secreted by F. magna and associated with the bacterial surface. Successful gene targeting has previously not been performed in anaerobic cocci, but in order to study the role of the SufA that is present at the bacterial surface, we constructed an F. magna mutant that expresses a truncated SufA... (More)
Finegoldia magna is a member of the normal human bacterial flora on the skin and other non-sterile body surfaces, but this anaerobic coccus is also an important opportunistic pathogen. SufA was the first F. magna proteinase to be isolated and characterized. Many bacterial pathogens interfere with different steps of blood coagulation, and here we describe how purified SufA efficiently and specifically cleaves fibrinogen in human plasma. SufA is both secreted by F. magna and associated with the bacterial surface. Successful gene targeting has previously not been performed in anaerobic cocci, but in order to study the role of the SufA that is present at the bacterial surface, we constructed an F. magna mutant that expresses a truncated SufA lacking proteolytic activity. In contrast to wild-type bacteria that delayed the coagulation of human plasma, mutant bacteria had no such effect. Wild-type and mutant bacteria adhered to keratinocytes equally well, but in a plasma environment only wild-type bacteria blocked the formation of fibrin networks surrounding adherent bacteria. The effective cleavage of fibrinogen by SufA suggests that the interference with fibrin network formation represents an adaptive mechanism of F. magna with potential implications also for pathogenicity. (Less)
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author
organization
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type
Contribution to journal
publication status
published
subject
in
Microbiology1994-01-01+01:00
volume
155
issue
Pt 1
pages
238 - 248
publisher
MAIK Nauka/Interperiodica
external identifiers
  • wos:000262730400025
  • pmid:19118364
  • scopus:61449101478
ISSN
1465-2080
DOI
10.1099/mic.0.021311-0
language
English
LU publication?
yes
id
64b25982-b2bb-4a96-9ce3-f4e343489bdd (old id 1290037)
alternative location
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2885652/
date added to LUP
2009-02-03 10:00:39
date last changed
2017-07-19 15:33:06
@article{64b25982-b2bb-4a96-9ce3-f4e343489bdd,
  abstract     = {Finegoldia magna is a member of the normal human bacterial flora on the skin and other non-sterile body surfaces, but this anaerobic coccus is also an important opportunistic pathogen. SufA was the first F. magna proteinase to be isolated and characterized. Many bacterial pathogens interfere with different steps of blood coagulation, and here we describe how purified SufA efficiently and specifically cleaves fibrinogen in human plasma. SufA is both secreted by F. magna and associated with the bacterial surface. Successful gene targeting has previously not been performed in anaerobic cocci, but in order to study the role of the SufA that is present at the bacterial surface, we constructed an F. magna mutant that expresses a truncated SufA lacking proteolytic activity. In contrast to wild-type bacteria that delayed the coagulation of human plasma, mutant bacteria had no such effect. Wild-type and mutant bacteria adhered to keratinocytes equally well, but in a plasma environment only wild-type bacteria blocked the formation of fibrin networks surrounding adherent bacteria. The effective cleavage of fibrinogen by SufA suggests that the interference with fibrin network formation represents an adaptive mechanism of F. magna with potential implications also for pathogenicity.},
  author       = {Karlsson, Christofer and Mörgelin, Matthias and Collin, Mattias and Lood, Rolf and Andersson, Marie-Louise and Schmidtchen, Artur and Björck, Lars and Frick, Inga-Maria},
  issn         = {1465-2080},
  language     = {eng},
  number       = {Pt 1},
  pages        = {238--248},
  publisher    = {MAIK Nauka/Interperiodica},
  series       = {Microbiology1994-01-01+01:00},
  title        = {SufA - a bacterial enzyme that cleaves fibrinogen and blocks fibrin network formation.},
  url          = {http://dx.doi.org/10.1099/mic.0.021311-0},
  volume       = {155},
  year         = {2009},
}