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Ability of antioxidants to prevent oxidative mutations in Salmonella typhimurium TA102,

Grey, Carl LU and Adlercreutz, Patrick LU (2003) In Mutation Research 527(1-2). p.27-36
Abstract
An assay for the ability of antioxidants to prevent mutations induced by various oxidants in Salmonella typhimurium TA102 cells was developed. Protection against hydrogen-peroxide-induced mutagenicity was observed for quercetin, caffeic acid, ascorbic acid and dimethyl sulfoxide (used as a solvent for water-insoluble antioxidants). No protective effect was observed for green tea extract (weakly pro-oxidative), catechin, rutin, sinigrin, ferulic acid and -tocopherol. Mutagenicity caused by tert-butyl hydroperoxide (tBOOH) was prevented most effectively by quercetin and ascorbic acid, whereas weaker effects were observed for green tea extract and for rutin, and no effect being observed for the other antioxidants tested. The results for... (More)
An assay for the ability of antioxidants to prevent mutations induced by various oxidants in Salmonella typhimurium TA102 cells was developed. Protection against hydrogen-peroxide-induced mutagenicity was observed for quercetin, caffeic acid, ascorbic acid and dimethyl sulfoxide (used as a solvent for water-insoluble antioxidants). No protective effect was observed for green tea extract (weakly pro-oxidative), catechin, rutin, sinigrin, ferulic acid and -tocopherol. Mutagenicity caused by tert-butyl hydroperoxide (tBOOH) was prevented most effectively by quercetin and ascorbic acid, whereas weaker effects were observed for green tea extract and for rutin, and no effect being observed for the other antioxidants tested. The results for hydrogen peroxide indicate iron chelation to be the most important protective mechanism. Radical scavenging appeared to be effective only with dimethyl sulfoxide and ascorbic acid, which are effective scavengers of hydroxyl radicals and were used here in high concentrations. It is proposed that the hydrogen-peroxide-induced mutations in the Salmonella cells are caused by hydroxyl radicals generated by iron ions closely associated with DNA. Protection against mutagenicity caused by tert-butyl hydroperoxide appears to occur mainly through the scavenging of alkoxyl and possibly of alkyl radicals. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
tert-Butyl hydroperoxide, Hydrogen peroxide, Oxidative DNA damage, Antioxidant, Salmonella typhimurium
in
Mutation Research
volume
527
issue
1-2
pages
27 - 36
publisher
Elsevier
external identifiers
  • pmid:12787911
  • wos:000183573400004
  • scopus:0037685148
ISSN
1873-135X
DOI
language
English
LU publication?
yes
id
c17d9810-1b3e-4112-8271-fece56ceff5c (old id 129126)
date added to LUP
2007-07-02 09:48:36
date last changed
2018-05-29 10:23:02
@article{c17d9810-1b3e-4112-8271-fece56ceff5c,
  abstract     = {An assay for the ability of antioxidants to prevent mutations induced by various oxidants in Salmonella typhimurium TA102 cells was developed. Protection against hydrogen-peroxide-induced mutagenicity was observed for quercetin, caffeic acid, ascorbic acid and dimethyl sulfoxide (used as a solvent for water-insoluble antioxidants). No protective effect was observed for green tea extract (weakly pro-oxidative), catechin, rutin, sinigrin, ferulic acid and -tocopherol. Mutagenicity caused by tert-butyl hydroperoxide (tBOOH) was prevented most effectively by quercetin and ascorbic acid, whereas weaker effects were observed for green tea extract and for rutin, and no effect being observed for the other antioxidants tested. The results for hydrogen peroxide indicate iron chelation to be the most important protective mechanism. Radical scavenging appeared to be effective only with dimethyl sulfoxide and ascorbic acid, which are effective scavengers of hydroxyl radicals and were used here in high concentrations. It is proposed that the hydrogen-peroxide-induced mutations in the Salmonella cells are caused by hydroxyl radicals generated by iron ions closely associated with DNA. Protection against mutagenicity caused by tert-butyl hydroperoxide appears to occur mainly through the scavenging of alkoxyl and possibly of alkyl radicals.},
  author       = {Grey, Carl and Adlercreutz, Patrick},
  issn         = {1873-135X},
  keyword      = {tert-Butyl hydroperoxide,Hydrogen peroxide,Oxidative DNA damage,Antioxidant,Salmonella typhimurium},
  language     = {eng},
  number       = {1-2},
  pages        = {27--36},
  publisher    = {Elsevier},
  series       = {Mutation Research},
  title        = {Ability of antioxidants to prevent oxidative mutations in Salmonella typhimurium TA102,},
  url          = {http://dx.doi.org/},
  volume       = {527},
  year         = {2003},
}