Ability of antioxidants to prevent oxidative mutations in Salmonella typhimurium TA102,
(2003) In Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis 527(1-2). p.27-36- Abstract
- An assay for the ability of antioxidants to prevent mutations induced by various oxidants in Salmonella typhimurium TA102 cells was developed. Protection against hydrogen-peroxide-induced mutagenicity was observed for quercetin, caffeic acid, ascorbic acid and dimethyl sulfoxide (used as a solvent for water-insoluble antioxidants). No protective effect was observed for green tea extract (weakly pro-oxidative), catechin, rutin, sinigrin, ferulic acid and -tocopherol. Mutagenicity caused by tert-butyl hydroperoxide (tBOOH) was prevented most effectively by quercetin and ascorbic acid, whereas weaker effects were observed for green tea extract and for rutin, and no effect being observed for the other antioxidants tested. The results for... (More)
- An assay for the ability of antioxidants to prevent mutations induced by various oxidants in Salmonella typhimurium TA102 cells was developed. Protection against hydrogen-peroxide-induced mutagenicity was observed for quercetin, caffeic acid, ascorbic acid and dimethyl sulfoxide (used as a solvent for water-insoluble antioxidants). No protective effect was observed for green tea extract (weakly pro-oxidative), catechin, rutin, sinigrin, ferulic acid and -tocopherol. Mutagenicity caused by tert-butyl hydroperoxide (tBOOH) was prevented most effectively by quercetin and ascorbic acid, whereas weaker effects were observed for green tea extract and for rutin, and no effect being observed for the other antioxidants tested. The results for hydrogen peroxide indicate iron chelation to be the most important protective mechanism. Radical scavenging appeared to be effective only with dimethyl sulfoxide and ascorbic acid, which are effective scavengers of hydroxyl radicals and were used here in high concentrations. It is proposed that the hydrogen-peroxide-induced mutations in the Salmonella cells are caused by hydroxyl radicals generated by iron ions closely associated with DNA. Protection against mutagenicity caused by tert-butyl hydroperoxide appears to occur mainly through the scavenging of alkoxyl and possibly of alkyl radicals. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/129126
- author
- Grey, Carl LU and Adlercreutz, Patrick LU
- organization
- publishing date
- 2003
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- tert-Butyl hydroperoxide, Hydrogen peroxide, Oxidative DNA damage, Antioxidant, Salmonella typhimurium
- in
- Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis
- volume
- 527
- issue
- 1-2
- pages
- 27 - 36
- publisher
- Elsevier
- external identifiers
-
- pmid:12787911
- wos:000183573400004
- scopus:0037685148
- ISSN
- 1879-2871
- DOI
- 10.1016/S0027-5107(03)00054-X
- language
- English
- LU publication?
- yes
- id
- c17d9810-1b3e-4112-8271-fece56ceff5c (old id 129126)
- date added to LUP
- 2016-04-01 17:03:04
- date last changed
- 2022-02-05 20:25:07
@article{c17d9810-1b3e-4112-8271-fece56ceff5c, abstract = {{An assay for the ability of antioxidants to prevent mutations induced by various oxidants in Salmonella typhimurium TA102 cells was developed. Protection against hydrogen-peroxide-induced mutagenicity was observed for quercetin, caffeic acid, ascorbic acid and dimethyl sulfoxide (used as a solvent for water-insoluble antioxidants). No protective effect was observed for green tea extract (weakly pro-oxidative), catechin, rutin, sinigrin, ferulic acid and -tocopherol. Mutagenicity caused by tert-butyl hydroperoxide (tBOOH) was prevented most effectively by quercetin and ascorbic acid, whereas weaker effects were observed for green tea extract and for rutin, and no effect being observed for the other antioxidants tested. The results for hydrogen peroxide indicate iron chelation to be the most important protective mechanism. Radical scavenging appeared to be effective only with dimethyl sulfoxide and ascorbic acid, which are effective scavengers of hydroxyl radicals and were used here in high concentrations. It is proposed that the hydrogen-peroxide-induced mutations in the Salmonella cells are caused by hydroxyl radicals generated by iron ions closely associated with DNA. Protection against mutagenicity caused by tert-butyl hydroperoxide appears to occur mainly through the scavenging of alkoxyl and possibly of alkyl radicals.}}, author = {{Grey, Carl and Adlercreutz, Patrick}}, issn = {{1879-2871}}, keywords = {{tert-Butyl hydroperoxide; Hydrogen peroxide; Oxidative DNA damage; Antioxidant; Salmonella typhimurium}}, language = {{eng}}, number = {{1-2}}, pages = {{27--36}}, publisher = {{Elsevier}}, series = {{Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis}}, title = {{Ability of antioxidants to prevent oxidative mutations in Salmonella typhimurium TA102,}}, url = {{http://dx.doi.org/10.1016/S0027-5107(03)00054-X}}, doi = {{10.1016/S0027-5107(03)00054-X}}, volume = {{527}}, year = {{2003}}, }