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Simultaneous detection of ethanol, glucose and glycerol in wines using PQQ - dependent dehydrogenase based biosensors

Niculescu, Mihaela; Mieliauskiene, Rasa; Laurinavicius, Valdas and Csöregi, Elisabeth LU (2003) In Food Chemistry 82(3). p.481-489
Abstract
Amperometric biosensors based on corresponding dehydrogenases have been developed for the determination of ethanol, glucose and glycerol. The enzymes have been integrated in redox hydrogels using an Os complex-modified non-conducting polymer employed as the electrochemical mediator and poly(ethyleneglycol)-diglycidyl ether (PEGDGE) as the cross-linking agent. The developed biosensors showed a sensitive response to ethanol, glucose and glycerol within the concentration range 2.5–250, 20–800, and 1–200 M, detection limits of 1.2, 9 and 1 M, and sensitivities of 220, 87 and 32 mA M−1 cm−2, respectively. The ethanol, glucose and glycerol content of several types of wine was determined with these biosensors, and the results were compared with... (More)
Amperometric biosensors based on corresponding dehydrogenases have been developed for the determination of ethanol, glucose and glycerol. The enzymes have been integrated in redox hydrogels using an Os complex-modified non-conducting polymer employed as the electrochemical mediator and poly(ethyleneglycol)-diglycidyl ether (PEGDGE) as the cross-linking agent. The developed biosensors showed a sensitive response to ethanol, glucose and glycerol within the concentration range 2.5–250, 20–800, and 1–200 M, detection limits of 1.2, 9 and 1 M, and sensitivities of 220, 87 and 32 mA M−1 cm−2, respectively. The ethanol, glucose and glycerol content of several types of wine was determined with these biosensors, and the results were compared with those obtained by spectrophotometric methods. The developed biosensors have been successfully employed for simultaneous determination of all these substrates at the required sensitivity. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Ethanol, Glucose, Glycerol, Biosensor, Wine analysis
in
Food Chemistry
volume
82
issue
3
pages
481 - 489
publisher
Elsevier
external identifiers
  • wos:000184047800021
  • scopus:0037671541
ISSN
1873-7072
DOI
10.1016/S0308-8146(03)00118-3
language
English
LU publication?
yes
id
658e03f7-cada-4ad4-b766-07aa51ddab08 (old id 129242)
date added to LUP
2007-07-02 15:29:19
date last changed
2018-02-04 03:57:39
@article{658e03f7-cada-4ad4-b766-07aa51ddab08,
  abstract     = {Amperometric biosensors based on corresponding dehydrogenases have been developed for the determination of ethanol, glucose and glycerol. The enzymes have been integrated in redox hydrogels using an Os complex-modified non-conducting polymer employed as the electrochemical mediator and poly(ethyleneglycol)-diglycidyl ether (PEGDGE) as the cross-linking agent. The developed biosensors showed a sensitive response to ethanol, glucose and glycerol within the concentration range 2.5–250, 20–800, and 1–200 M, detection limits of 1.2, 9 and 1 M, and sensitivities of 220, 87 and 32 mA M−1 cm−2, respectively. The ethanol, glucose and glycerol content of several types of wine was determined with these biosensors, and the results were compared with those obtained by spectrophotometric methods. The developed biosensors have been successfully employed for simultaneous determination of all these substrates at the required sensitivity.},
  author       = {Niculescu, Mihaela and Mieliauskiene, Rasa and Laurinavicius, Valdas and Csöregi, Elisabeth},
  issn         = {1873-7072},
  keyword      = {Ethanol,Glucose,Glycerol,Biosensor,Wine analysis},
  language     = {eng},
  number       = {3},
  pages        = {481--489},
  publisher    = {Elsevier},
  series       = {Food Chemistry},
  title        = {Simultaneous detection of ethanol, glucose and glycerol in wines using PQQ - dependent dehydrogenase based biosensors},
  url          = {http://dx.doi.org/10.1016/S0308-8146(03)00118-3},
  volume       = {82},
  year         = {2003},
}