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Effects of 17alpha-ethinylestradiol on immune parameters in the lizard Sceloporus occidentalis

Burnham, D Kim; Lackey, Amanda; Manering, Michelle; Jaensson Gyllenbäck, Elin LU ; Pearson, Jamie; Tyler, Daniel O; Melson, David and Talent, Larry G (2003) In Environmental Toxicology 18(4). p.211-218
Abstract
We examined the effect of 17alpha-ethinylestradiol on immunity of the Western fence lizard, Sceloporus occidentalis. Injection of 17alpha-ethinylestradiol resulted in dose-dependent suppression of peripheral blood leukocyte levels as determined by cell counts, whereas total spleen cell levels were decreased only at higher doses of 17alpha-ethinylestradiol. In contrast, spleen cell proliferation was enhanced by 17alpha-ethinylestradiol as measured by reduction of MTT to formazan following a two-way mixed lymphocyte reaction. Antibody responses were unaffected. Effects on peripheral blood leukocyte levels and spleen cell proliferation similar to those observed in response to injection of 17alpha-ethinylestradiol were observed following... (More)
We examined the effect of 17alpha-ethinylestradiol on immunity of the Western fence lizard, Sceloporus occidentalis. Injection of 17alpha-ethinylestradiol resulted in dose-dependent suppression of peripheral blood leukocyte levels as determined by cell counts, whereas total spleen cell levels were decreased only at higher doses of 17alpha-ethinylestradiol. In contrast, spleen cell proliferation was enhanced by 17alpha-ethinylestradiol as measured by reduction of MTT to formazan following a two-way mixed lymphocyte reaction. Antibody responses were unaffected. Effects on peripheral blood leukocyte levels and spleen cell proliferation similar to those observed in response to injection of 17alpha-ethinylestradiol were observed following injection of a single dose of hydrocortisone. However, injection of lizards with 17alpha-ethinylestradiol did not result in a significant increase in serum cortisol. Results of this study suggest that exposure of Western fence lizards to 17alpha-ethinylestradiol leads to decreased numbers of circulating leukocytes and total spleen cell numbers and the enhancement of spleen cell proliferation in a two-way mixed lymphocyte reaction. These effects probably involve mechanisms other than or in addition to the induction of cortisol release. (Less)
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author
publishing date
type
Contribution to journal
publication status
published
subject
in
Environmental Toxicology
volume
18
issue
4
pages
211 - 218
publisher
John Wiley & Sons
external identifiers
  • wos:000184632600001
  • scopus:0042978779
ISSN
1520-4081
DOI
10.1002/tox.10117
language
English
LU publication?
no
id
63616822-673a-4fd0-9f99-a235af661940 (old id 1297619)
date added to LUP
2009-07-14 09:16:25
date last changed
2018-09-02 03:24:30
@article{63616822-673a-4fd0-9f99-a235af661940,
  abstract     = {We examined the effect of 17alpha-ethinylestradiol on immunity of the Western fence lizard, Sceloporus occidentalis. Injection of 17alpha-ethinylestradiol resulted in dose-dependent suppression of peripheral blood leukocyte levels as determined by cell counts, whereas total spleen cell levels were decreased only at higher doses of 17alpha-ethinylestradiol. In contrast, spleen cell proliferation was enhanced by 17alpha-ethinylestradiol as measured by reduction of MTT to formazan following a two-way mixed lymphocyte reaction. Antibody responses were unaffected. Effects on peripheral blood leukocyte levels and spleen cell proliferation similar to those observed in response to injection of 17alpha-ethinylestradiol were observed following injection of a single dose of hydrocortisone. However, injection of lizards with 17alpha-ethinylestradiol did not result in a significant increase in serum cortisol. Results of this study suggest that exposure of Western fence lizards to 17alpha-ethinylestradiol leads to decreased numbers of circulating leukocytes and total spleen cell numbers and the enhancement of spleen cell proliferation in a two-way mixed lymphocyte reaction. These effects probably involve mechanisms other than or in addition to the induction of cortisol release.},
  author       = {Burnham, D Kim and Lackey, Amanda and Manering, Michelle and Jaensson Gyllenbäck, Elin and Pearson, Jamie and Tyler, Daniel O and Melson, David and Talent, Larry G},
  issn         = {1520-4081},
  language     = {eng},
  number       = {4},
  pages        = {211--218},
  publisher    = {John Wiley & Sons},
  series       = {Environmental Toxicology},
  title        = {Effects of 17alpha-ethinylestradiol on immune parameters in the lizard Sceloporus occidentalis},
  url          = {http://dx.doi.org/10.1002/tox.10117},
  volume       = {18},
  year         = {2003},
}