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Mass overloading in the flow field-flow fractionation channel studied by the behaviour of the ultra-large wheat protein glutein.

Arfvidsson, Cecilia LU and Wahlund, Karl-Gustav LU (2003) In Journal of Chromatography A 1011(1-2). p.99-109
Abstract
Flow field-flow fractionation (FFF) has previously been used in successful fractionation and characterisation of the ultra-large wheat protein glutenin. The many parameters, which may influence the retention behaviour, especially when analysing extremely high-molecular-mass samples such as glutenin, are here reported. Size determination from the sample retention time, using FFF theory, will as a result have a very low accuracy. The need for direct molecular mass determination, such as by light scattering, in combination with FFF, in order to do accurate size measurements of glutenin is pointed out as well as the importance to minimise the overloading.
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Chromatography A
volume
1011
issue
1-2
pages
99 - 109
publisher
Elsevier
external identifiers
  • wos:000185157500011
  • pmid:14518767
  • scopus:0042521032
ISSN
0021-9673
DOI
language
English
LU publication?
yes
id
743ac15a-dd00-4641-992c-fe907bd3785a (old id 132717)
date added to LUP
2007-06-26 12:37:47
date last changed
2018-05-29 10:41:41
@article{743ac15a-dd00-4641-992c-fe907bd3785a,
  abstract     = {Flow field-flow fractionation (FFF) has previously been used in successful fractionation and characterisation of the ultra-large wheat protein glutenin. The many parameters, which may influence the retention behaviour, especially when analysing extremely high-molecular-mass samples such as glutenin, are here reported. Size determination from the sample retention time, using FFF theory, will as a result have a very low accuracy. The need for direct molecular mass determination, such as by light scattering, in combination with FFF, in order to do accurate size measurements of glutenin is pointed out as well as the importance to minimise the overloading.},
  author       = {Arfvidsson, Cecilia and Wahlund, Karl-Gustav},
  issn         = {0021-9673},
  language     = {eng},
  number       = {1-2},
  pages        = {99--109},
  publisher    = {Elsevier},
  series       = {Journal of Chromatography A},
  title        = {Mass overloading in the flow field-flow fractionation channel studied by the behaviour of the ultra-large wheat protein glutein.},
  url          = {http://dx.doi.org/},
  volume       = {1011},
  year         = {2003},
}