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Prevalence of Clostridium botulinum types B, E, and F in faecal samples from Swedish cattle.

Dahlenborg, Maria; Borch, Elisabeth and Rådström, Peter LU (2003) In International Journal of Food Microbiology 82(2). p.105-110
Abstract
Faeces were collected from 60 cows at three slaughterhouses situated in southern and central Sweden. The faecal samples were collected during two sampling periods over the year, summer and winter. All samples were analysed for the presence of Clostridium botulinum spores, according to a combined selection and enrichment PCR procedure. One PCR assay was specific for part of the type B neurotoxin gene, while the other assay was specific for both type E and F neurotoxin genes. The prevalence of C. botulinum in Swedish cattle was established to be 73% for non-proteolytic type B and less than 5% for types E and F. Twenty-eight (64%) of the positive faecal samples had a spore load of less than 4 spores/g. Statistical analysis (ANOVA) showed that... (More)
Faeces were collected from 60 cows at three slaughterhouses situated in southern and central Sweden. The faecal samples were collected during two sampling periods over the year, summer and winter. All samples were analysed for the presence of Clostridium botulinum spores, according to a combined selection and enrichment PCR procedure. One PCR assay was specific for part of the type B neurotoxin gene, while the other assay was specific for both type E and F neurotoxin genes. The prevalence of C. botulinum in Swedish cattle was established to be 73% for non-proteolytic type B and less than 5% for types E and F. Twenty-eight (64%) of the positive faecal samples had a spore load of less than 4 spores/g. Statistical analysis (ANOVA) showed that seasonal variation (summer and winter) had a significant effect on the prevalence of C. botulinum type B in cattle, whereas the effect of geographical location of rearing of the cattle (southern and central Sweden) was less significant. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Cattle, Prevalence, Faecal samples, Clostridium botulinum
in
International Journal of Food Microbiology
volume
82
issue
2
pages
105 - 110
publisher
Elsevier
external identifiers
  • pmid:12568750
  • wos:000181786700002
  • scopus:12244291208
ISSN
0168-1605
DOI
language
English
LU publication?
yes
id
e8cc49da-ea1d-4b5a-9e42-c024707cec3a (old id 132777)
date added to LUP
2007-06-28 11:37:50
date last changed
2018-05-29 10:24:32
@article{e8cc49da-ea1d-4b5a-9e42-c024707cec3a,
  abstract     = {Faeces were collected from 60 cows at three slaughterhouses situated in southern and central Sweden. The faecal samples were collected during two sampling periods over the year, summer and winter. All samples were analysed for the presence of Clostridium botulinum spores, according to a combined selection and enrichment PCR procedure. One PCR assay was specific for part of the type B neurotoxin gene, while the other assay was specific for both type E and F neurotoxin genes. The prevalence of C. botulinum in Swedish cattle was established to be 73% for non-proteolytic type B and less than 5% for types E and F. Twenty-eight (64%) of the positive faecal samples had a spore load of less than 4 spores/g. Statistical analysis (ANOVA) showed that seasonal variation (summer and winter) had a significant effect on the prevalence of C. botulinum type B in cattle, whereas the effect of geographical location of rearing of the cattle (southern and central Sweden) was less significant.},
  author       = {Dahlenborg, Maria and Borch, Elisabeth and Rådström, Peter},
  issn         = {0168-1605},
  keyword      = {Cattle,Prevalence,Faecal samples,Clostridium botulinum},
  language     = {eng},
  number       = {2},
  pages        = {105--110},
  publisher    = {Elsevier},
  series       = {International Journal of Food Microbiology},
  title        = {Prevalence of Clostridium botulinum types B, E, and F in faecal samples from Swedish cattle.},
  url          = {http://dx.doi.org/},
  volume       = {82},
  year         = {2003},
}