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High capacity xylose transport in Candida intermedia PYCC4715.

Gárdonyi, Márk LU ; Österberg, Måns; Rodrigues, C; Spencer-Martins, I and Hahn-Hägerdal, Bärbel LU (2003) In FEMS Yeast Research 3(1). p.45-52
Abstract
Xylose-utilising yeasts were screened to identify strains with high xylose transport capacity. Among the fastest-growing strains in xylose medium, Candida intermedia PYCC 4715 showed the highest xylose transport capacity. Maximal specific growth rate was the same in glucose and xylose media (small mu, Greekmax=0.5 h−1, 30°C). Xylose transport showed biphasic kinetics when cells were grown in either xylose- or glucose-limited culture. The high-affinity xylose/proton symport system (Km=0.2 mM, Vmax=7.5 mmol h−1 g−1) was more repressed by glucose than by xylose. The less specific low-affinity transport system (K=50 mM, Vmax=11 mmol h−1 g−1) appeared to operate through a facilitated-diffusion mechanism and was expressed constitutively.... (More)
Xylose-utilising yeasts were screened to identify strains with high xylose transport capacity. Among the fastest-growing strains in xylose medium, Candida intermedia PYCC 4715 showed the highest xylose transport capacity. Maximal specific growth rate was the same in glucose and xylose media (small mu, Greekmax=0.5 h−1, 30°C). Xylose transport showed biphasic kinetics when cells were grown in either xylose- or glucose-limited culture. The high-affinity xylose/proton symport system (Km=0.2 mM, Vmax=7.5 mmol h−1 g−1) was more repressed by glucose than by xylose. The less specific low-affinity transport system (K=50 mM, Vmax=11 mmol h−1 g−1) appeared to operate through a facilitated-diffusion mechanism and was expressed constitutively. Inhibition experiments showed that glucose is a substrate of both xylose transport systems. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
FEMS Yeast Research
volume
3
issue
1
pages
45 - 52
publisher
Elsevier
external identifiers
  • wos:000181502500007
  • scopus:0037369565
ISSN
1567-1364
DOI
10.1016/S1567-1356(02)00137-X
language
English
LU publication?
yes
id
8c48c7f9-5e14-4ff0-b45d-825a3560e4ba (old id 132782)
date added to LUP
2007-06-28 11:57:15
date last changed
2017-05-28 03:34:57
@article{8c48c7f9-5e14-4ff0-b45d-825a3560e4ba,
  abstract     = {Xylose-utilising yeasts were screened to identify strains with high xylose transport capacity. Among the fastest-growing strains in xylose medium, Candida intermedia PYCC 4715 showed the highest xylose transport capacity. Maximal specific growth rate was the same in glucose and xylose media (small mu, Greekmax=0.5 h−1, 30°C). Xylose transport showed biphasic kinetics when cells were grown in either xylose- or glucose-limited culture. The high-affinity xylose/proton symport system (Km=0.2 mM, Vmax=7.5 mmol h−1 g−1) was more repressed by glucose than by xylose. The less specific low-affinity transport system (K=50 mM, Vmax=11 mmol h−1 g−1) appeared to operate through a facilitated-diffusion mechanism and was expressed constitutively. Inhibition experiments showed that glucose is a substrate of both xylose transport systems.},
  author       = {Gárdonyi, Márk and Österberg, Måns and Rodrigues, C and Spencer-Martins, I and Hahn-Hägerdal, Bärbel},
  issn         = {1567-1364},
  language     = {eng},
  number       = {1},
  pages        = {45--52},
  publisher    = {Elsevier},
  series       = {FEMS Yeast Research},
  title        = {High capacity xylose transport in Candida intermedia PYCC4715.},
  url          = {http://dx.doi.org/10.1016/S1567-1356(02)00137-X},
  volume       = {3},
  year         = {2003},
}