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Standardization of diagnostic PCR for the detection of foodborne pathogens.

Malorny, B; Tassios, P T; Rådström, Peter LU ; Cook, N; Wagner, M and Hoorfar, J (2003) In International Journal of Food Microbiology 83(1). p.39-48
Abstract
In vitro amplification of nucleic acids using the polymerase chain reaction (PCR) has become, since its discovery in the 1980s, a powerful diagnostic tool for the analysis of microbial infections as well as for the analysis of microorganisms in food samples. However, despite its potential, PCR has neither gained wide acceptance in routine diagnostics nor been widely incorporated in standardized methods. Lack of validation and standard protocols, as well as variable quality of reagents and equipment, influence the efficient dissemination of PCR methodology from expert research laboratories to end-user laboratories. Moreover, the food industry understandably requires and expects officially approved standards. Recognizing this, in 1999, the... (More)
In vitro amplification of nucleic acids using the polymerase chain reaction (PCR) has become, since its discovery in the 1980s, a powerful diagnostic tool for the analysis of microbial infections as well as for the analysis of microorganisms in food samples. However, despite its potential, PCR has neither gained wide acceptance in routine diagnostics nor been widely incorporated in standardized methods. Lack of validation and standard protocols, as well as variable quality of reagents and equipment, influence the efficient dissemination of PCR methodology from expert research laboratories to end-user laboratories. Moreover, the food industry understandably requires and expects officially approved standards. Recognizing this, in 1999, the European Commission approved the research project, FOOD-PCR (http://www.PCR.dk), which aims to validate and standardize the use of diagnostic PCR for the detection of pathogenic bacteria in foods. The present review focuses on the harmonization procedure and standardization criteria for detection of foodborne pathogens by PCR. The progress of standardization so far and future perspectives of diagnostic PCR are discussed. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
PCR, Harmonization and standardization, Detection, Foodborne pathogens
in
International Journal of Food Microbiology
volume
83
issue
1
pages
39 - 48
publisher
Elsevier
external identifiers
  • wos:000182692300004
  • pmid:12672591
  • scopus:0037466726
ISSN
0168-1605
DOI
language
English
LU publication?
yes
id
d9a7f958-97c2-415b-b0d7-1f447cd75932 (old id 132820)
date added to LUP
2007-06-28 16:18:45
date last changed
2018-06-17 03:58:26
@article{d9a7f958-97c2-415b-b0d7-1f447cd75932,
  abstract     = {In vitro amplification of nucleic acids using the polymerase chain reaction (PCR) has become, since its discovery in the 1980s, a powerful diagnostic tool for the analysis of microbial infections as well as for the analysis of microorganisms in food samples. However, despite its potential, PCR has neither gained wide acceptance in routine diagnostics nor been widely incorporated in standardized methods. Lack of validation and standard protocols, as well as variable quality of reagents and equipment, influence the efficient dissemination of PCR methodology from expert research laboratories to end-user laboratories. Moreover, the food industry understandably requires and expects officially approved standards. Recognizing this, in 1999, the European Commission approved the research project, FOOD-PCR (http://www.PCR.dk), which aims to validate and standardize the use of diagnostic PCR for the detection of pathogenic bacteria in foods. The present review focuses on the harmonization procedure and standardization criteria for detection of foodborne pathogens by PCR. The progress of standardization so far and future perspectives of diagnostic PCR are discussed.},
  author       = {Malorny, B and Tassios, P T and Rådström, Peter and Cook, N and Wagner, M and Hoorfar, J},
  issn         = {0168-1605},
  keyword      = {PCR,Harmonization and standardization,Detection,Foodborne pathogens},
  language     = {eng},
  number       = {1},
  pages        = {39--48},
  publisher    = {Elsevier},
  series       = {International Journal of Food Microbiology},
  title        = {Standardization of diagnostic PCR for the detection of foodborne pathogens.},
  url          = {http://dx.doi.org/},
  volume       = {83},
  year         = {2003},
}