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Effects of ultraviolet-radiation on viability of isolated beta-vulgaris and hordeum-vulgare protoplasts

Bornman, Janet LU ; Bornman, C H and Björn, Lars Olof LU (1982) In Zeitschrift für Pflanzenphysiologie 105(4). p.297-306
Abstract
Estimates of viability as measured by vital staining with fluorescein diacetate (FDA) were carried out on freshly isolated and partially aged (16-h-old) B. vulgaris and H. vulgare mesophyll protoplasts following irradiation with UV-B. Damage to the photosynthetic system by UV-B was determined by delayed light emission (DLE). In the case of freshly isolated protoplasts Beta was .apprx. 30% more susceptible than Hordeum following 3 h irradiation, with viability decreasing from 90% to 40%. After storage of protoplasts on ice for 16 h UV-B radiation markedly depressed viability in both species, but in the case of Hordeum there was a substantial initial loss of nearly 70% in viability over the 1st hour of irradiation. The first 10 min of UV-B... (More)
Estimates of viability as measured by vital staining with fluorescein diacetate (FDA) were carried out on freshly isolated and partially aged (16-h-old) B. vulgaris and H. vulgare mesophyll protoplasts following irradiation with UV-B. Damage to the photosynthetic system by UV-B was determined by delayed light emission (DLE). In the case of freshly isolated protoplasts Beta was .apprx. 30% more susceptible than Hordeum following 3 h irradiation, with viability decreasing from 90% to 40%. After storage of protoplasts on ice for 16 h UV-B radiation markedly depressed viability in both species, but in the case of Hordeum there was a substantial initial loss of nearly 70% in viability over the 1st hour of irradiation. The first 10 min of UV-B radiation decreased the intensity of DLE by 40% without appreciably affecting the decay rate. Longer treatment times did not give a proportional effect so that even after 60 min of UV-B the inhibition did not exceed 60%. This suggested that although the enzyme system responsible for FDA hydrolysis may be partially inactivated (viability was 75-80% as compared with 90% in the control), the UV-B did not penetrate the innermost parts of the chloroplasts, but left some thylakoids undamaged. (Less)
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publication status
published
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in
Zeitschrift für Pflanzenphysiologie
volume
105
issue
4
pages
297 - 306
publisher
Gustav Fischer Verlag
ISSN
0044-328X
language
English
LU publication?
yes
id
5bf3b6c7-efb6-4f12-bc66-2b5480bf4563 (old id 134623)
date added to LUP
2007-07-30 09:51:56
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2016-04-16 03:31:55
@article{5bf3b6c7-efb6-4f12-bc66-2b5480bf4563,
  abstract     = {Estimates of viability as measured by vital staining with fluorescein diacetate (FDA) were carried out on freshly isolated and partially aged (16-h-old) B. vulgaris and H. vulgare mesophyll protoplasts following irradiation with UV-B. Damage to the photosynthetic system by UV-B was determined by delayed light emission (DLE). In the case of freshly isolated protoplasts Beta was .apprx. 30% more susceptible than Hordeum following 3 h irradiation, with viability decreasing from 90% to 40%. After storage of protoplasts on ice for 16 h UV-B radiation markedly depressed viability in both species, but in the case of Hordeum there was a substantial initial loss of nearly 70% in viability over the 1st hour of irradiation. The first 10 min of UV-B radiation decreased the intensity of DLE by 40% without appreciably affecting the decay rate. Longer treatment times did not give a proportional effect so that even after 60 min of UV-B the inhibition did not exceed 60%. This suggested that although the enzyme system responsible for FDA hydrolysis may be partially inactivated (viability was 75-80% as compared with 90% in the control), the UV-B did not penetrate the innermost parts of the chloroplasts, but left some thylakoids undamaged.},
  author       = {Bornman, Janet and Bornman, C H and Björn, Lars Olof},
  issn         = {0044-328X},
  language     = {eng},
  number       = {4},
  pages        = {297--306},
  publisher    = {Gustav Fischer Verlag},
  series       = {Zeitschrift für Pflanzenphysiologie},
  title        = {Effects of ultraviolet-radiation on viability of isolated beta-vulgaris and hordeum-vulgare protoplasts},
  volume       = {105},
  year         = {1982},
}