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Humoral Immune Response Against Defined Oxidized Low-Density Lipoprotein Antigens Reflects Structure and Disease Activity of Carotid Plaques.

Goncalves, Isabel LU ; Gronholdt, Marie-Louise M; Söderberg, Ingrid LU ; Ares, Mikko LU ; Nordestgaard, Borge G; Bentzon, Jacob F; Nordin Fredrikson, Gunilla LU and Nilsson, Jan LU (2005) In Arteriosclerosis, Thrombosis and Vascular Biology 25(6). p.1250-1255
Abstract
Background - Immune responses against oxidized low- density lipoprotein ( LDL) play an important role in atherosclerosis. The aim of this study was to investigate if humoral immune response against specific oxidized LDL antigens, such as aldehyde- modified peptide sequences of apolipoprotein B- 100, reflects disease activity and structure of atherosclerotic plaques. Methods and Results - Plaques were obtained from 114 symptomatic subjects referred to carotid endarterectomy and characterized immunohistochemically and histologically. Plasma levels of IgG and IgM against aldehyde- modified apolipoprotein B- 100 amino acid sequences 661 to 680, 3136 to 3155 ( peptide 210), and 3661 to 3680 ( peptide 240) were determined by enzyme- linked... (More)
Background - Immune responses against oxidized low- density lipoprotein ( LDL) play an important role in atherosclerosis. The aim of this study was to investigate if humoral immune response against specific oxidized LDL antigens, such as aldehyde- modified peptide sequences of apolipoprotein B- 100, reflects disease activity and structure of atherosclerotic plaques. Methods and Results - Plaques were obtained from 114 symptomatic subjects referred to carotid endarterectomy and characterized immunohistochemically and histologically. Plasma levels of IgG and IgM against aldehyde- modified apolipoprotein B- 100 amino acid sequences 661 to 680, 3136 to 3155 ( peptide 210), and 3661 to 3680 ( peptide 240) were determined by enzyme- linked immunosorbent assay. High levels of IgG against peptide 210 were associated with increased plaque content of lipids ( r = 0.24, P < 0.05) and hemorrhage ( r = 0.27, P = 0.005), with decreased content of fibrous tissue ( r = - 0.25, P = 0.01), but also with lower total plaque volume ( r = - 0.21, P < 0.05). In contrast, high levels of IgM against peptide 240 were associated with plaques with more fibrous tissue ( r = 0.35, P < 0.001), less lipids ( r = - 0.34, P < 0.001), and less macrophages ( r = - 0.24, P < 0.05). IgM against peptide 210 were found to be associated with plaque fibrous tissue ( r = 0.20, P < 0.05), less lipids ( r = - 0.21, P < 0.05), and less macrophages ( r = - 0.27, P = 0.01). Conclusion - These findings support the notion that immune responses against oxidized LDL epitopes are involved in atherosclerosis and that the level of circulating antibodies against these structures may reflect disease activity in the arterial wall. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
atherosclerosis, low-density lipoprotein, antibodies, modified, carotid stenosis, carotid plaque
in
Arteriosclerosis, Thrombosis and Vascular Biology
volume
25
issue
6
pages
1250 - 1255
publisher
American Heart Association
external identifiers
  • pmid:15831809
  • wos:000229465000029
  • scopus:20144378591
ISSN
1524-4636
DOI
10.1161/01.ATV.0000166518.96137.38
language
English
LU publication?
yes
id
9ae7b9e1-e7f1-48c4-aeed-bdc36e28ce7b (old id 136199)
alternative location
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=15831809&dopt=Abstract
date added to LUP
2007-07-20 12:38:05
date last changed
2017-10-29 03:41:38
@article{9ae7b9e1-e7f1-48c4-aeed-bdc36e28ce7b,
  abstract     = {Background - Immune responses against oxidized low- density lipoprotein ( LDL) play an important role in atherosclerosis. The aim of this study was to investigate if humoral immune response against specific oxidized LDL antigens, such as aldehyde- modified peptide sequences of apolipoprotein B- 100, reflects disease activity and structure of atherosclerotic plaques. Methods and Results - Plaques were obtained from 114 symptomatic subjects referred to carotid endarterectomy and characterized immunohistochemically and histologically. Plasma levels of IgG and IgM against aldehyde- modified apolipoprotein B- 100 amino acid sequences 661 to 680, 3136 to 3155 ( peptide 210), and 3661 to 3680 ( peptide 240) were determined by enzyme- linked immunosorbent assay. High levels of IgG against peptide 210 were associated with increased plaque content of lipids ( r = 0.24, P &lt; 0.05) and hemorrhage ( r = 0.27, P = 0.005), with decreased content of fibrous tissue ( r = - 0.25, P = 0.01), but also with lower total plaque volume ( r = - 0.21, P &lt; 0.05). In contrast, high levels of IgM against peptide 240 were associated with plaques with more fibrous tissue ( r = 0.35, P &lt; 0.001), less lipids ( r = - 0.34, P &lt; 0.001), and less macrophages ( r = - 0.24, P &lt; 0.05). IgM against peptide 210 were found to be associated with plaque fibrous tissue ( r = 0.20, P &lt; 0.05), less lipids ( r = - 0.21, P &lt; 0.05), and less macrophages ( r = - 0.27, P = 0.01). Conclusion - These findings support the notion that immune responses against oxidized LDL epitopes are involved in atherosclerosis and that the level of circulating antibodies against these structures may reflect disease activity in the arterial wall.},
  author       = {Goncalves, Isabel and Gronholdt, Marie-Louise M and Söderberg, Ingrid and Ares, Mikko and Nordestgaard, Borge G and Bentzon, Jacob F and Nordin Fredrikson, Gunilla and Nilsson, Jan},
  issn         = {1524-4636},
  keyword      = {atherosclerosis,low-density lipoprotein,antibodies,modified,carotid stenosis,carotid plaque},
  language     = {eng},
  number       = {6},
  pages        = {1250--1255},
  publisher    = {American Heart Association},
  series       = {Arteriosclerosis, Thrombosis and Vascular Biology},
  title        = {Humoral Immune Response Against Defined Oxidized Low-Density Lipoprotein Antigens Reflects Structure and Disease Activity of Carotid Plaques.},
  url          = {http://dx.doi.org/10.1161/01.ATV.0000166518.96137.38},
  volume       = {25},
  year         = {2005},
}