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Autochthonous versus allochthonous carbon sources of bacteria: Results from whole-lake C-13 addition experiments

Kritzberg, Emma LU ; Cole, J J; Pace, M L; Granéli, Wilhelm LU and Bade, D L (2004) In Limnology and Oceanography 49(2). p.588-596
Abstract
Organic substrates for pelagic bacteria are derived from dissolved organic carbon (DOC) in the water column. DOC is a heterogeneous mixture of molecules, some of which are imported from the watershed (allochthonous DOC) and others that are produced by autotrophs within the system (autochthonous DOC). We examined the importance of autochthonous versus allochthonous DOC in supporting the growth of pelagic bacteria by manipulating the C-13 content of autochthonous sources in a whole-lake experiment. (NaHCO3)-C-13 was added daily to two small forested lakes for a period of 42 d, thereby strongly labeling autochthonous primary production. To obtain bacterial carbon isotopes, bacteria were regrown in vitro in particle-free lake water and in situ... (More)
Organic substrates for pelagic bacteria are derived from dissolved organic carbon (DOC) in the water column. DOC is a heterogeneous mixture of molecules, some of which are imported from the watershed (allochthonous DOC) and others that are produced by autotrophs within the system (autochthonous DOC). We examined the importance of autochthonous versus allochthonous DOC in supporting the growth of pelagic bacteria by manipulating the C-13 content of autochthonous sources in a whole-lake experiment. (NaHCO3)-C-13 was added daily to two small forested lakes for a period of 42 d, thereby strongly labeling autochthonous primary production. To obtain bacterial carbon isotopes, bacteria were regrown in vitro in particle-free lake water and in situ in dialysis tubes; little difference was found between the two methods. The contribution of autochthonous versus allochthonous carbon to the bacterial biomass was estimated by applying a two-member mixing model using a C-13 of -28parts per thousand as the allochthonous end member. The autochthonous end member, which varied over time, was estimated indirectly by several approaches. The bacterial biomass consisted of 35-70% allochthonous carbon. This result confirms the often-stated hypothesis that autochthonous carbon alone does not support bacterial production. On the other hand, autochthonous DOC was preferentially utilized relative to terrestrial DOC. On the basis of C-13 measurements, only 13% of the DOC standing stock was of recent autochthonous origin, but it supported 30-65% of bacterial production. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Limnology and Oceanography
volume
49
issue
2
pages
588 - 596
publisher
ASLO
external identifiers
  • wos:000224979500026
ISSN
1939-5590
language
English
LU publication?
yes
id
5328cee1-d2c2-4cd8-b48c-f7434d1270ca (old id 136594)
alternative location
http://80-www.aslo.org/lo/toc/vol_49/issue_2/0588.pdf
date added to LUP
2007-06-28 14:13:49
date last changed
2016-04-16 05:03:19
@article{5328cee1-d2c2-4cd8-b48c-f7434d1270ca,
  abstract     = {Organic substrates for pelagic bacteria are derived from dissolved organic carbon (DOC) in the water column. DOC is a heterogeneous mixture of molecules, some of which are imported from the watershed (allochthonous DOC) and others that are produced by autotrophs within the system (autochthonous DOC). We examined the importance of autochthonous versus allochthonous DOC in supporting the growth of pelagic bacteria by manipulating the C-13 content of autochthonous sources in a whole-lake experiment. (NaHCO3)-C-13 was added daily to two small forested lakes for a period of 42 d, thereby strongly labeling autochthonous primary production. To obtain bacterial carbon isotopes, bacteria were regrown in vitro in particle-free lake water and in situ in dialysis tubes; little difference was found between the two methods. The contribution of autochthonous versus allochthonous carbon to the bacterial biomass was estimated by applying a two-member mixing model using a C-13 of -28parts per thousand as the allochthonous end member. The autochthonous end member, which varied over time, was estimated indirectly by several approaches. The bacterial biomass consisted of 35-70% allochthonous carbon. This result confirms the often-stated hypothesis that autochthonous carbon alone does not support bacterial production. On the other hand, autochthonous DOC was preferentially utilized relative to terrestrial DOC. On the basis of C-13 measurements, only 13% of the DOC standing stock was of recent autochthonous origin, but it supported 30-65% of bacterial production.},
  author       = {Kritzberg, Emma and Cole, J J and Pace, M L and Granéli, Wilhelm and Bade, D L},
  issn         = {1939-5590},
  language     = {eng},
  number       = {2},
  pages        = {588--596},
  publisher    = {ASLO},
  series       = {Limnology and Oceanography},
  title        = {Autochthonous versus allochthonous carbon sources of bacteria: Results from whole-lake C-13 addition experiments},
  volume       = {49},
  year         = {2004},
}