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Nanospray mass spectrometry with indirect conductive graphite coating

Viberg, Peter LU ; Nilsson, Staffan LU and Skog, Kerstin LU (2004) In Analytical Chemistry 76(14). p.4241-4244
Abstract
An easy and cost-effective method to manufacture a robust conductive graphite coating for nanospray mass spectrometry (nESI-MS) and capillary electrophoresis (CE)-nESI-MS is described. The method involves graphite coating of a tube sleeve, into which the nESI emitter is inserted and connected to a transfer capillary, instead of coating the actual emitter. The coating, made of graphite from a pencil and epoxy glue, was stable over long periods of use (>80 h) and showed excellent resistance toward various solvents. Stable electrospray was achieved in the investigated flow range (150-900 nL min(-1)), and salbutamol, diphenhydramine, and nortriptyline (M-w: 239-263 g mol(-1)) were detected in the nanomole per liter range during continuous... (More)
An easy and cost-effective method to manufacture a robust conductive graphite coating for nanospray mass spectrometry (nESI-MS) and capillary electrophoresis (CE)-nESI-MS is described. The method involves graphite coating of a tube sleeve, into which the nESI emitter is inserted and connected to a transfer capillary, instead of coating the actual emitter. The coating, made of graphite from a pencil and epoxy glue, was stable over long periods of use (>80 h) and showed excellent resistance toward various solvents. Stable electrospray was achieved in the investigated flow range (150-900 nL min(-1)), and salbutamol, diphenhydramine, and nortriptyline (M-w: 239-263 g mol(-1)) were detected in the nanomole per liter range during continuous pumping. CE-nESI-MS analysis gave excellent signal-to-noise ratios for 100-fmol injections. The technique allows simple exchange of the nESI emitter to suit a specific flow rate, and it minimizes risk of corona discharge. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Analytical Chemistry
volume
76
issue
14
pages
4241 - 4244
publisher
The American Chemical Society
external identifiers
  • wos:000222706400052
  • pmid:15253670
  • scopus:3242676968
ISSN
1520-6882
DOI
10.1021/ac049728e
language
English
LU publication?
yes
id
89c9ece1-7ea2-4d87-981c-5b00a7ffa238 (old id 138612)
date added to LUP
2007-06-27 16:25:44
date last changed
2017-01-01 04:56:24
@article{89c9ece1-7ea2-4d87-981c-5b00a7ffa238,
  abstract     = {An easy and cost-effective method to manufacture a robust conductive graphite coating for nanospray mass spectrometry (nESI-MS) and capillary electrophoresis (CE)-nESI-MS is described. The method involves graphite coating of a tube sleeve, into which the nESI emitter is inserted and connected to a transfer capillary, instead of coating the actual emitter. The coating, made of graphite from a pencil and epoxy glue, was stable over long periods of use (>80 h) and showed excellent resistance toward various solvents. Stable electrospray was achieved in the investigated flow range (150-900 nL min(-1)), and salbutamol, diphenhydramine, and nortriptyline (M-w: 239-263 g mol(-1)) were detected in the nanomole per liter range during continuous pumping. CE-nESI-MS analysis gave excellent signal-to-noise ratios for 100-fmol injections. The technique allows simple exchange of the nESI emitter to suit a specific flow rate, and it minimizes risk of corona discharge.},
  author       = {Viberg, Peter and Nilsson, Staffan and Skog, Kerstin},
  issn         = {1520-6882},
  language     = {eng},
  number       = {14},
  pages        = {4241--4244},
  publisher    = {The American Chemical Society},
  series       = {Analytical Chemistry},
  title        = {Nanospray mass spectrometry with indirect conductive graphite coating},
  url          = {http://dx.doi.org/10.1021/ac049728e},
  volume       = {76},
  year         = {2004},
}