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In-capillary micro solid-phase extraction and capillary electrophoresis separation of heterocyclic aromatic amines with nanospray mass spectrometric detection

Viberg, Peter LU ; Nilsson, Staffan LU and Skog, Kerstin LU (2004) In Analytical and Bioanalytical Chemistry 378(7). p.1729-1734
Abstract
A miniaturised technique to analyse and detect heterocyclic aromatic amines (HAs) using micro solid-phase extraction (muSPE) coupled on-line (in-capillary) to capillary electrophoresis (CE) separation with nanospray (nESI) mass spectrometry (MS) detection has been developed. HAs are mutagenic and carcinogenic compounds formed at low levels in protein-rich food during cooking. Due to the low concentrations of HAs and the high complexity of the matrix in which they exist, sensitive and selective analytical methods are required for quantification. muSPE was performed on a packed bed of C-18 particles inside the CE capillary, which minimised the dead volume. The on-line coupling of SPE, CE and nESI-MS reduced the time for extraction and... (More)
A miniaturised technique to analyse and detect heterocyclic aromatic amines (HAs) using micro solid-phase extraction (muSPE) coupled on-line (in-capillary) to capillary electrophoresis (CE) separation with nanospray (nESI) mass spectrometry (MS) detection has been developed. HAs are mutagenic and carcinogenic compounds formed at low levels in protein-rich food during cooking. Due to the low concentrations of HAs and the high complexity of the matrix in which they exist, sensitive and selective analytical methods are required for quantification. muSPE was performed on a packed bed of C-18 particles inside the CE capillary, which minimised the dead volume. The on-line coupling of SPE, CE and nESI-MS reduced the time for extraction and identification to less than half an hour, which will allow for screening of several samples per day. The new technique provides short analysis time, low sample and solvent consumption, and HAs in standard solutions were easily detected at 12-17 fmol injections, and in spiked urine samples at 750-810 fmol injections. (Less)
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author
organization
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type
Contribution to journal
publication status
published
subject
in
Analytical and Bioanalytical Chemistry
volume
378
issue
7
pages
1729 - 1734
publisher
Springer
external identifiers
  • wos:000220458400011
  • pmid:14747890
  • scopus:3242732799
ISSN
1618-2642
DOI
10.1007/s00216-003-2480-2
language
English
LU publication?
yes
id
4a4f5374-c5ac-4649-86db-bd4b8fe7ca12 (old id 138623)
date added to LUP
2007-06-28 10:58:05
date last changed
2017-04-30 08:27:02
@article{4a4f5374-c5ac-4649-86db-bd4b8fe7ca12,
  abstract     = {A miniaturised technique to analyse and detect heterocyclic aromatic amines (HAs) using micro solid-phase extraction (muSPE) coupled on-line (in-capillary) to capillary electrophoresis (CE) separation with nanospray (nESI) mass spectrometry (MS) detection has been developed. HAs are mutagenic and carcinogenic compounds formed at low levels in protein-rich food during cooking. Due to the low concentrations of HAs and the high complexity of the matrix in which they exist, sensitive and selective analytical methods are required for quantification. muSPE was performed on a packed bed of C-18 particles inside the CE capillary, which minimised the dead volume. The on-line coupling of SPE, CE and nESI-MS reduced the time for extraction and identification to less than half an hour, which will allow for screening of several samples per day. The new technique provides short analysis time, low sample and solvent consumption, and HAs in standard solutions were easily detected at 12-17 fmol injections, and in spiked urine samples at 750-810 fmol injections.},
  author       = {Viberg, Peter and Nilsson, Staffan and Skog, Kerstin},
  issn         = {1618-2642},
  language     = {eng},
  number       = {7},
  pages        = {1729--1734},
  publisher    = {Springer},
  series       = {Analytical and Bioanalytical Chemistry},
  title        = {In-capillary micro solid-phase extraction and capillary electrophoresis separation of heterocyclic aromatic amines with nanospray mass spectrometric detection},
  url          = {http://dx.doi.org/10.1007/s00216-003-2480-2},
  volume       = {378},
  year         = {2004},
}