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Neutron reflectometry to investigate the delivery of lipids and DNA to interfaces

Nylander, Tommy LU ; Campbell, Richard A.; Vandoolaeghe, Pauline LU ; Cardenas, Marite; Linse, Per LU and Rennie, Adrian R. (2008) In Biointerphases 3(2). p.64-82
Abstract
The application of scattering methods in the study of biological and biomedical problems is a field of research that is currently experiencing fast growth. In particular, neutron reflectometry (NR) is a technique that is becoming progressively more widespread, as indicated by the current commissioning of several new reflectometers worldwide. NR is valuable for the characterization of biomolecules at interfaces due to its capability to provide quantitative structural and compositional information on relevant molecular length scales. Recent years have seen an increasing number of applications of NR to problems related to drug and gene delivery. We start our review by summarizing the experimental methodology of the technique with reference to... (More)
The application of scattering methods in the study of biological and biomedical problems is a field of research that is currently experiencing fast growth. In particular, neutron reflectometry (NR) is a technique that is becoming progressively more widespread, as indicated by the current commissioning of several new reflectometers worldwide. NR is valuable for the characterization of biomolecules at interfaces due to its capability to provide quantitative structural and compositional information on relevant molecular length scales. Recent years have seen an increasing number of applications of NR to problems related to drug and gene delivery. We start our review by summarizing the experimental methodology of the technique with reference to the description of biological liquid interfaces. Various methods for the interpretation of data are then discussed, including a new approach based on the lattice mean-field theory to help characterize stimulus-responsive surfaces relevant to drug delivery function. Recent progress in the subject area is reviewed in terms of NR studies relevant to the delivery of lipids and DNA to surfaces. Lastly, we discuss two case studies to exemplify practical features of NR that are exploited in combination with complementary techniques. The first case concerns the interactions of lipid-based cubic phase nanoparticles with model membranes (a drug delivery application), and the second case concerns DNA compaction at surfaces and in the bulk solution (a gene delivery application). (C) 2008 American Vacuum Society. [DOI: 10.1116/1.2976448] (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Biointerphases
volume
3
issue
2
pages
64 - 82
publisher
AVS
external identifiers
  • wos:000264979100028
  • scopus:77952928034
ISSN
1934-8630
DOI
10.1116/1.2976448
language
English
LU publication?
yes
id
1f1ac9b6-352b-46f4-b04a-53f8e361cea0 (old id 1399311)
date added to LUP
2009-05-28 13:51:04
date last changed
2017-07-30 03:31:44
@article{1f1ac9b6-352b-46f4-b04a-53f8e361cea0,
  abstract     = {The application of scattering methods in the study of biological and biomedical problems is a field of research that is currently experiencing fast growth. In particular, neutron reflectometry (NR) is a technique that is becoming progressively more widespread, as indicated by the current commissioning of several new reflectometers worldwide. NR is valuable for the characterization of biomolecules at interfaces due to its capability to provide quantitative structural and compositional information on relevant molecular length scales. Recent years have seen an increasing number of applications of NR to problems related to drug and gene delivery. We start our review by summarizing the experimental methodology of the technique with reference to the description of biological liquid interfaces. Various methods for the interpretation of data are then discussed, including a new approach based on the lattice mean-field theory to help characterize stimulus-responsive surfaces relevant to drug delivery function. Recent progress in the subject area is reviewed in terms of NR studies relevant to the delivery of lipids and DNA to surfaces. Lastly, we discuss two case studies to exemplify practical features of NR that are exploited in combination with complementary techniques. The first case concerns the interactions of lipid-based cubic phase nanoparticles with model membranes (a drug delivery application), and the second case concerns DNA compaction at surfaces and in the bulk solution (a gene delivery application). (C) 2008 American Vacuum Society. [DOI: 10.1116/1.2976448]},
  author       = {Nylander, Tommy and Campbell, Richard A. and Vandoolaeghe, Pauline and Cardenas, Marite and Linse, Per and Rennie, Adrian R.},
  issn         = {1934-8630},
  language     = {eng},
  number       = {2},
  pages        = {64--82},
  publisher    = {AVS},
  series       = {Biointerphases},
  title        = {Neutron reflectometry to investigate the delivery of lipids and DNA to interfaces},
  url          = {http://dx.doi.org/10.1116/1.2976448},
  volume       = {3},
  year         = {2008},
}