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Reduced hippocampal neurogenesis in R6/2 transgenic Huntington's disease mice.

Gil, Joana LU ; Mohapel, Paul LU ; Araujo, Ines LU ; Popovic, Natalija LU ; Li, Jia-Yi LU ; Brundin, Patrik LU and Petersén, Åsa LU (2005) In Neurobiology of Disease 20(3). p.744-751
Abstract
We investigated whether cell proliferation and neurogenesis are altered in R6/2 transgenic Huntington's disease mice. Using bromodeoxyuridine (BrdU), we found a progressive decrease in the number of proliferating cells in the dentate gyrus of R6/2 mice. This reduction was detected in pre-symptomatic mice, and by 11.5 weeks, R6/2 mice had 66% fewer newly born cells in the hippocampus. The results were confirmed by immunohistochemistry for the cell cycle markers Ki-67 and proliferating cell nuclear antigen (PCNA). We did not observe changes in cell proliferation in the R6/2 subventricular zone, indicating that the decrease in cell proliferation is specific for the hippocampus. This decrease corresponded to a reduction in actual hippocampal... (More)
We investigated whether cell proliferation and neurogenesis are altered in R6/2 transgenic Huntington's disease mice. Using bromodeoxyuridine (BrdU), we found a progressive decrease in the number of proliferating cells in the dentate gyrus of R6/2 mice. This reduction was detected in pre-symptomatic mice, and by 11.5 weeks, R6/2 mice had 66% fewer newly born cells in the hippocampus. The results were confirmed by immunohistochemistry for the cell cycle markers Ki-67 and proliferating cell nuclear antigen (PCNA). We did not observe changes in cell proliferation in the R6/2 subventricular zone, indicating that the decrease in cell proliferation is specific for the hippocampus. This decrease corresponded to a reduction in actual hippocampal neurogenesis as assessed by double immunostaining for BrdU and the neuronal marker neuronal nuclei (NeuN) and by immunohistochemistry for the neuroblast marker doublecortin. Reduced hippocampal neurogenesis may be a novel neuropathological feature in R6/2 mice that could be assessed when evaluating potential therapies. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Cell proliferation, Dentate gyrus, Doublecortin, Huntington's disease, Ki-67, Neurogenesis, R6/2 mice, PCNA, Subventricular zone, BrdU
in
Neurobiology of Disease
volume
20
issue
3
pages
744 - 751
publisher
Elsevier
external identifiers
  • wos:000233739100013
  • pmid:15951191
  • scopus:27644485740
ISSN
0969-9961
DOI
10.1016/j.nbd.2005.05.006
language
English
LU publication?
yes
id
67b2862a-c1f3-42e7-bd1e-c345b97e43f6 (old id 140069)
alternative location
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=15951191&query_hl=14006
date added to LUP
2007-07-12 13:39:18
date last changed
2017-11-12 03:17:47
@article{67b2862a-c1f3-42e7-bd1e-c345b97e43f6,
  abstract     = {We investigated whether cell proliferation and neurogenesis are altered in R6/2 transgenic Huntington's disease mice. Using bromodeoxyuridine (BrdU), we found a progressive decrease in the number of proliferating cells in the dentate gyrus of R6/2 mice. This reduction was detected in pre-symptomatic mice, and by 11.5 weeks, R6/2 mice had 66% fewer newly born cells in the hippocampus. The results were confirmed by immunohistochemistry for the cell cycle markers Ki-67 and proliferating cell nuclear antigen (PCNA). We did not observe changes in cell proliferation in the R6/2 subventricular zone, indicating that the decrease in cell proliferation is specific for the hippocampus. This decrease corresponded to a reduction in actual hippocampal neurogenesis as assessed by double immunostaining for BrdU and the neuronal marker neuronal nuclei (NeuN) and by immunohistochemistry for the neuroblast marker doublecortin. Reduced hippocampal neurogenesis may be a novel neuropathological feature in R6/2 mice that could be assessed when evaluating potential therapies.},
  author       = {Gil, Joana and Mohapel, Paul and Araujo, Ines and Popovic, Natalija and Li, Jia-Yi and Brundin, Patrik and Petersén, Åsa},
  issn         = {0969-9961},
  keyword      = {Cell proliferation,Dentate gyrus,Doublecortin,Huntington's disease,Ki-67,Neurogenesis,R6/2 mice,PCNA,Subventricular zone,BrdU},
  language     = {eng},
  number       = {3},
  pages        = {744--751},
  publisher    = {Elsevier},
  series       = {Neurobiology of Disease},
  title        = {Reduced hippocampal neurogenesis in R6/2 transgenic Huntington's disease mice.},
  url          = {http://dx.doi.org/10.1016/j.nbd.2005.05.006},
  volume       = {20},
  year         = {2005},
}