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Quantitative analysis of the generation of different striatal neuronal subtypes in the adult brain following excitotoxic injury.

Collin, Tove LU ; Arvidsson, Andreas LU ; Kokaia, Zaal LU and Lindvall, Olle LU (2005) In Experimental Neurology 195(1). p.71-80
Abstract
Recent findings in adult rodents have provided evidence for the formation of new striatal neurons from subventricular zone (SVZ) precursors following stroke. Little is known about which factors determine the magnitude of striatal neurogenesis in the damaged brain. Here we studied striatal neurogenesis following an excitotoxic lesion to the adult rat striatum induced by intrastriatal quinolinic acid (QA) infusion. New cells were labeled with the thymidine-analogue 5-bromo-2'-deoxyuridine (BrdU) and their identity was determined immunocytochemically with various phenotypic markers. The unilateral lesion gave rise to increased cell proliferation mainly in the ipsilateral SVZ. At 2 weeks following the insult, there was a pronounced increase of... (More)
Recent findings in adult rodents have provided evidence for the formation of new striatal neurons from subventricular zone (SVZ) precursors following stroke. Little is known about which factors determine the magnitude of striatal neurogenesis in the damaged brain. Here we studied striatal neurogenesis following an excitotoxic lesion to the adult rat striatum induced by intrastriatal quinolinic acid (QA) infusion. New cells were labeled with the thymidine-analogue 5-bromo-2'-deoxyuridine (BrdU) and their identity was determined immunocytochemically with various phenotypic markers. The unilateral lesion gave rise to increased cell proliferation mainly in the ipsilateral SVZ. At 2 weeks following the insult, there was a pronounced increase of the number of new neurons co-expressing BrdU and a marker of migrating neuroblasts, doublecortin, in the ipsilateral striatum, particularly its non-damaged medial parts. About 80% of the new neurons survived up to 6 weeks, when they expressed the mature neuronal marker NeuN and were preferentially located in the outer parts of the damaged area. Lesion-generated neurons expressed phenotypic markers of striatal medium spiny neurons (DARPP-32) and intemeurons (parvalbumin or neuropeptide Y). The magnitude of neurogenesis correlated to the size of the striatal damage. Our data show for the first time that an excitotoxic lesion to the striatum can trigger the formation of new striatal neurons with phenotypes of both projection neurons and interneurons. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
rat striatum, neurogenesis, excitotoxic injury
in
Experimental Neurology
volume
195
issue
1
pages
71 - 80
publisher
Academic Press
external identifiers
  • pmid:15936016
  • wos:000231556300008
  • scopus:23644456973
ISSN
0014-4886
DOI
10.1016/j.expneurol.2005.03.017
language
English
LU publication?
yes
id
bea121ea-a714-4eb2-b366-e1291335f544 (old id 140244)
alternative location
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=15936016&query_hl=13
date added to LUP
2007-07-13 12:12:27
date last changed
2017-09-24 03:36:27
@article{bea121ea-a714-4eb2-b366-e1291335f544,
  abstract     = {Recent findings in adult rodents have provided evidence for the formation of new striatal neurons from subventricular zone (SVZ) precursors following stroke. Little is known about which factors determine the magnitude of striatal neurogenesis in the damaged brain. Here we studied striatal neurogenesis following an excitotoxic lesion to the adult rat striatum induced by intrastriatal quinolinic acid (QA) infusion. New cells were labeled with the thymidine-analogue 5-bromo-2'-deoxyuridine (BrdU) and their identity was determined immunocytochemically with various phenotypic markers. The unilateral lesion gave rise to increased cell proliferation mainly in the ipsilateral SVZ. At 2 weeks following the insult, there was a pronounced increase of the number of new neurons co-expressing BrdU and a marker of migrating neuroblasts, doublecortin, in the ipsilateral striatum, particularly its non-damaged medial parts. About 80% of the new neurons survived up to 6 weeks, when they expressed the mature neuronal marker NeuN and were preferentially located in the outer parts of the damaged area. Lesion-generated neurons expressed phenotypic markers of striatal medium spiny neurons (DARPP-32) and intemeurons (parvalbumin or neuropeptide Y). The magnitude of neurogenesis correlated to the size of the striatal damage. Our data show for the first time that an excitotoxic lesion to the striatum can trigger the formation of new striatal neurons with phenotypes of both projection neurons and interneurons.},
  author       = {Collin, Tove and Arvidsson, Andreas and Kokaia, Zaal and Lindvall, Olle},
  issn         = {0014-4886},
  keyword      = {rat striatum,neurogenesis,excitotoxic injury},
  language     = {eng},
  number       = {1},
  pages        = {71--80},
  publisher    = {Academic Press},
  series       = {Experimental Neurology},
  title        = {Quantitative analysis of the generation of different striatal neuronal subtypes in the adult brain following excitotoxic injury.},
  url          = {http://dx.doi.org/10.1016/j.expneurol.2005.03.017},
  volume       = {195},
  year         = {2005},
}