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Importance of polyamines in cell cycle kinetics as studied in a transgenic system.

Nasizadeh, Sima LU ; Myhre, Louise LU ; Thiman, Lena LU ; Alm, Kersti LU ; Oredsson, Stina LU and Persson, Lo LU (2005) In Experimental Cell Research 308(May 26). p.254-264
Abstract
Polyamines are organic cations, which are considered essential for normal cell cycle progression. This view is based on results from numerous studies using a variety of enzyme inhibitors or polyamine analogues interfering with either the metabolism or the physiological functions of the polyamines. However, the presence of non-specific effects may be hard to rule out in such studies. In the present study, we have for the first time used a transgenic cell system to analyze the importance of polyamines in cell growth. We have earlier shown that expression of trypanosomal ODC in an ODC-deficient variant of CHO cells (C55.7) supported growth of these otherwise polyamine auxotrophic cells. However, one of the transgenic cell lines grew much... (More)
Polyamines are organic cations, which are considered essential for normal cell cycle progression. This view is based on results from numerous studies using a variety of enzyme inhibitors or polyamine analogues interfering with either the metabolism or the physiological functions of the polyamines. However, the presence of non-specific effects may be hard to rule out in such studies. In the present study, we have for the first time used a transgenic cell system to analyze the importance of polyamines in cell growth. We have earlier shown that expression of trypanosomal ODC in an ODC-deficient variant of CHO cells (C55.7) supported growth of these otherwise polyamine auxotrophic cells. However, one of the transgenic cell lines grew much slower than the others. As shown in the present study, the level of ODC activity was much lower in these cells, and that was reflected in a reduction of cellular polyamine levels. Analysis of cell cycle kinetics revealed that reduction of growth was correlated to prolongation of the G(1), S, and G(2) + M phases in the cells. Providing exogenous putrescine to the cells resulted in a normalization of polyamine levels as well as cell cycle kinetics indicating a causal relationship. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
polyamines, ornithine decarboxylase, trypanosomal, cyclins, cell growth, cell cycle
in
Experimental Cell Research
volume
308
issue
May 26
pages
254 - 264
publisher
Academic Press
external identifiers
  • pmid:15923003
  • wos:000231099100002
  • scopus:24944479210
ISSN
1090-2422
DOI
10.1016/j.yexcr.2005.04.027
language
English
LU publication?
yes
id
8bb3383c-fe25-4ff5-919a-9f99c52c5f52 (old id 140438)
alternative location
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=15923003&query_hl=102
date added to LUP
2007-07-18 12:31:03
date last changed
2017-01-01 04:22:45
@article{8bb3383c-fe25-4ff5-919a-9f99c52c5f52,
  abstract     = {Polyamines are organic cations, which are considered essential for normal cell cycle progression. This view is based on results from numerous studies using a variety of enzyme inhibitors or polyamine analogues interfering with either the metabolism or the physiological functions of the polyamines. However, the presence of non-specific effects may be hard to rule out in such studies. In the present study, we have for the first time used a transgenic cell system to analyze the importance of polyamines in cell growth. We have earlier shown that expression of trypanosomal ODC in an ODC-deficient variant of CHO cells (C55.7) supported growth of these otherwise polyamine auxotrophic cells. However, one of the transgenic cell lines grew much slower than the others. As shown in the present study, the level of ODC activity was much lower in these cells, and that was reflected in a reduction of cellular polyamine levels. Analysis of cell cycle kinetics revealed that reduction of growth was correlated to prolongation of the G(1), S, and G(2) + M phases in the cells. Providing exogenous putrescine to the cells resulted in a normalization of polyamine levels as well as cell cycle kinetics indicating a causal relationship.},
  author       = {Nasizadeh, Sima and Myhre, Louise and Thiman, Lena and Alm, Kersti and Oredsson, Stina and Persson, Lo},
  issn         = {1090-2422},
  keyword      = {polyamines,ornithine decarboxylase,trypanosomal,cyclins,cell growth,cell cycle},
  language     = {eng},
  number       = {May 26},
  pages        = {254--264},
  publisher    = {Academic Press},
  series       = {Experimental Cell Research},
  title        = {Importance of polyamines in cell cycle kinetics as studied in a transgenic system.},
  url          = {http://dx.doi.org/10.1016/j.yexcr.2005.04.027},
  volume       = {308},
  year         = {2005},
}