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Pancreatic juice protects gut from pathogenic bacteria

Kruszewska, Danuta LU ; Ljungh, Åsa LU and Pierzynowski, Stefan LU (2003) International Symposium on Energy and Protein Metabolism and Nutrition In Progress in Research on Energy and Protein Metabolism 109. p.303-305
Abstract
The role of the exocrine pancreas in regulating gut microflora colonization is unclear. The main objective in the present study was to assess the affect of pancreatic fluid on the growth of pathogenic bacteria and fungi. Pancreatic juice samples used in the study were obtained from nine eight-week old weaned pigs in which catheters were implanted in the pancreatic duct. The antibacterial activity of pure pig pancreatic juice collected from healthy, conscious and also anaesthetized pigs was investigated with multi-resistant microbial isolates and non-pathogenic strains. Studies were performed on 23 bacterial and 2 Candida albicans isolates, including 4 lactic acid bacteria (LAB) and 3 reference strains. Pancreatic juice was effective... (More)
The role of the exocrine pancreas in regulating gut microflora colonization is unclear. The main objective in the present study was to assess the affect of pancreatic fluid on the growth of pathogenic bacteria and fungi. Pancreatic juice samples used in the study were obtained from nine eight-week old weaned pigs in which catheters were implanted in the pancreatic duct. The antibacterial activity of pure pig pancreatic juice collected from healthy, conscious and also anaesthetized pigs was investigated with multi-resistant microbial isolates and non-pathogenic strains. Studies were performed on 23 bacterial and 2 Candida albicans isolates, including 4 lactic acid bacteria (LAB) and 3 reference strains. Pancreatic juice was effective (p<0.01) against multidrug resistant bacterial pathogens, whereas other strains had only moderate sensitivity (p<0.05) to its antibacterial action and furthermore LAB were insensitive. The antibacterial action was independent of pancreatic juice proteolytic activity and stable when measured before and after enterokinase activation of trypsinogen. We demonstrated in vitro, that the antibacterial properties of pancreatic juice last for several hours. Our data suggests that broth composition may modulate the intensity of pancreatic juice antibacterial activity in vitro; this can have implications for digestive related antibacterial activity in vivo. Thus, pancreatic juice antibacterial activity may be an important factor in limiting the colonization of pathogenic bacteria in the gastrointestinal tract, in both the small and the large intestines. We postulate that observed antibacterial activity of the pancreatic juice could play an important role as one of the factor of innate immunity. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
subject
keywords
pancreas, innate immunity
in
Progress in Research on Energy and Protein Metabolism
volume
109
pages
303 - 305
publisher
Wageningen Academic Publishers
conference name
International Symposium on Energy and Protein Metabolism and Nutrition
external identifiers
  • wos:000186518200052
ISSN
0071-2477
ISBN
978-90-76998-24-4
language
English
LU publication?
yes
id
d10fede6-b3cd-4c21-9dc1-1417ab61667d (old id 1407082)
date added to LUP
2009-06-02 10:47:52
date last changed
2018-05-29 12:01:39
@inproceedings{d10fede6-b3cd-4c21-9dc1-1417ab61667d,
  abstract     = {The role of the exocrine pancreas in regulating gut microflora colonization is unclear. The main objective in the present study was to assess the affect of pancreatic fluid on the growth of pathogenic bacteria and fungi. Pancreatic juice samples used in the study were obtained from nine eight-week old weaned pigs in which catheters were implanted in the pancreatic duct. The antibacterial activity of pure pig pancreatic juice collected from healthy, conscious and also anaesthetized pigs was investigated with multi-resistant microbial isolates and non-pathogenic strains. Studies were performed on 23 bacterial and 2 Candida albicans isolates, including 4 lactic acid bacteria (LAB) and 3 reference strains. Pancreatic juice was effective (p&lt;0.01) against multidrug resistant bacterial pathogens, whereas other strains had only moderate sensitivity (p&lt;0.05) to its antibacterial action and furthermore LAB were insensitive. The antibacterial action was independent of pancreatic juice proteolytic activity and stable when measured before and after enterokinase activation of trypsinogen. We demonstrated in vitro, that the antibacterial properties of pancreatic juice last for several hours. Our data suggests that broth composition may modulate the intensity of pancreatic juice antibacterial activity in vitro; this can have implications for digestive related antibacterial activity in vivo. Thus, pancreatic juice antibacterial activity may be an important factor in limiting the colonization of pathogenic bacteria in the gastrointestinal tract, in both the small and the large intestines. We postulate that observed antibacterial activity of the pancreatic juice could play an important role as one of the factor of innate immunity.},
  author       = {Kruszewska, Danuta and Ljungh, Åsa and Pierzynowski, Stefan},
  booktitle    = {Progress in Research on Energy and Protein Metabolism},
  isbn         = {978-90-76998-24-4},
  issn         = {0071-2477},
  keyword      = {pancreas,innate immunity},
  language     = {eng},
  pages        = {303--305},
  publisher    = {Wageningen Academic Publishers},
  title        = {Pancreatic juice protects gut from pathogenic bacteria},
  volume       = {109},
  year         = {2003},
}