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Absence of striatal newborn neurons with mature phenotype following defined striatal and cortical excitotoxic brain injuries.

Deierborg, Tomas LU ; Staflin, Karin LU ; Pesic, Jelena; Roybon, Laurent LU ; Brundin, Patrik LU and Lundberg, Cecilia LU (2009) In Experimental Neurology 219. p.363-367
Abstract
Experimental stroke and excitotoxic brain lesion to the striatum or cortex increase the proliferation of cells residing within the ventricular wall and cause subsequent migration of newborn neuroblasts into the lesioned brain parenchyma. In this study, we clarify the different events of neurogenesis following striatal or cortical excitotoxic brain lesions in adult rats. Newborn cells were labeled by intraperitoneal injection of bromo-deoxy-uridine (BrdU), or by green fluorescent protein (GFP)-expressing lentiviral vectors injected into the subventricular zone (SVZ). We show that only neural progenitors born the first 5 days in the SVZ reside and expand within this neurogenic niche over time, and that these early labeled cells are more... (More)
Experimental stroke and excitotoxic brain lesion to the striatum or cortex increase the proliferation of cells residing within the ventricular wall and cause subsequent migration of newborn neuroblasts into the lesioned brain parenchyma. In this study, we clarify the different events of neurogenesis following striatal or cortical excitotoxic brain lesions in adult rats. Newborn cells were labeled by intraperitoneal injection of bromo-deoxy-uridine (BrdU), or by green fluorescent protein (GFP)-expressing lentiviral vectors injected into the subventricular zone (SVZ). We show that only neural progenitors born the first 5 days in the SVZ reside and expand within this neurogenic niche over time, and that these early labeled cells are more prone to migrate towards the striatum as neuroblasts. However, these neuroblasts could not mature into NeuN(+) neurons in the striatum. Furthermore, we found that cortical lesions, close or distant from the SVZ, could not upregulate SVZ cell proliferation nor promote neurogenesis. Our study demonstrates that both the time window for labeling proliferating cells and the site of lesion are crucial when assessing neurogenesis following brain injury. (Less)
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author
organization
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type
Contribution to journal
publication status
published
subject
in
Experimental Neurology
volume
219
pages
363 - 367
publisher
Academic Press
external identifiers
  • wos:000269398600043
  • pmid:19427853
  • scopus:68749099422
ISSN
0014-4886
DOI
10.1016/j.expneurol.2009.05.002
language
English
LU publication?
yes
id
683a6ca5-b32f-4758-9bd2-e247f7361ea1 (old id 1412520)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/19427853?dopt=Abstract
date added to LUP
2009-06-02 09:06:07
date last changed
2017-07-31 10:20:20
@article{683a6ca5-b32f-4758-9bd2-e247f7361ea1,
  abstract     = {Experimental stroke and excitotoxic brain lesion to the striatum or cortex increase the proliferation of cells residing within the ventricular wall and cause subsequent migration of newborn neuroblasts into the lesioned brain parenchyma. In this study, we clarify the different events of neurogenesis following striatal or cortical excitotoxic brain lesions in adult rats. Newborn cells were labeled by intraperitoneal injection of bromo-deoxy-uridine (BrdU), or by green fluorescent protein (GFP)-expressing lentiviral vectors injected into the subventricular zone (SVZ). We show that only neural progenitors born the first 5 days in the SVZ reside and expand within this neurogenic niche over time, and that these early labeled cells are more prone to migrate towards the striatum as neuroblasts. However, these neuroblasts could not mature into NeuN(+) neurons in the striatum. Furthermore, we found that cortical lesions, close or distant from the SVZ, could not upregulate SVZ cell proliferation nor promote neurogenesis. Our study demonstrates that both the time window for labeling proliferating cells and the site of lesion are crucial when assessing neurogenesis following brain injury.},
  author       = {Deierborg, Tomas and Staflin, Karin and Pesic, Jelena and Roybon, Laurent and Brundin, Patrik and Lundberg, Cecilia},
  issn         = {0014-4886},
  language     = {eng},
  pages        = {363--367},
  publisher    = {Academic Press},
  series       = {Experimental Neurology},
  title        = {Absence of striatal newborn neurons with mature phenotype following defined striatal and cortical excitotoxic brain injuries.},
  url          = {http://dx.doi.org/10.1016/j.expneurol.2009.05.002},
  volume       = {219},
  year         = {2009},
}