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Arabidopsis RADICAL-INDUCED CELL DEATH1 is involved in UV-B signaling.

Jiang, Lei; Wang, Yan; Björn, Lars Olof LU and Li, Shaoshan (2009) In Photochemical and Photobiological Sciences 8. p.838-846
Abstract
The Arabidopsis radical-induced cell death1 (rcd1) mutant is sensitive to ozone fumigation and

apoplastic superoxide, but tolerant to methyl viologen. In the present article, we report that the rcd1

mutant is also tolerant to supplementary UV-B radiation. The rcd1-1 mutant exhibits less accumulation

of TT dimers, increased hypocotyl growth inhibition and higher accumulation of flavonoids under

supplemental UV-B radiation. Moreover, the expression of HY5 (ELONGATED HYPOCOTYL5) is

increased in the mutant after UV-B treatment. Gene expression downstream of UV-B signaling reveals

that COP1 (CONSTITUTIVELY PHOTOMORPHOGENIC1)-regulated genes have an elevated

expression in rcd1-1... (More)
The Arabidopsis radical-induced cell death1 (rcd1) mutant is sensitive to ozone fumigation and

apoplastic superoxide, but tolerant to methyl viologen. In the present article, we report that the rcd1

mutant is also tolerant to supplementary UV-B radiation. The rcd1-1 mutant exhibits less accumulation

of TT dimers, increased hypocotyl growth inhibition and higher accumulation of flavonoids under

supplemental UV-B radiation. Moreover, the expression of HY5 (ELONGATED HYPOCOTYL5) is

increased in the mutant after UV-B treatment. Gene expression downstream of UV-B signaling reveals

that COP1 (CONSTITUTIVELY PHOTOMORPHOGENIC1)-regulated genes have an elevated

expression in rcd1-1 mutant under UV-B radiation, while expression of UVR8 (UV RESISTANCE

LOCUS 8)-regulated and HY5-independent genes are not changed. Interestingly, the expression of

RCD1 genes is not significantly changed by UV-B radiation. Previous study has shown that STO

protein is interacting with RCD1 in vitro. Here, we found the mRNA level of STO (SALT

TOLERANCE) is greatly increased in rcd1-1 mutant after UV-B radiation. However, UV-B-induced

HY5 and CHS expression is partially inhibited in sto mutant. Based on the above results, it is deduced

that the RCD1, working together with STO, is involved in Arabidopsis UV-B signaling. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
UV-B, ultraviolet radiation, signaling, Arabidopis, rcd1
in
Photochemical and Photobiological Sciences
volume
8
pages
838 - 846
publisher
Royal Society of Chemistry
external identifiers
  • wos:000266598000013
  • scopus:67449151623
ISSN
1474-9092
DOI
10.1039/b901187k
project
Photobiology
language
English
LU publication?
yes
id
5cb6a7fd-4ef2-4551-a72d-8e9edb52a218 (old id 1419952)
date added to LUP
2009-06-22 12:31:42
date last changed
2017-11-19 03:29:27
@article{5cb6a7fd-4ef2-4551-a72d-8e9edb52a218,
  abstract     = {The Arabidopsis radical-induced cell death1 (rcd1) mutant is sensitive to ozone fumigation and<br/><br>
apoplastic superoxide, but tolerant to methyl viologen. In the present article, we report that the rcd1<br/><br>
mutant is also tolerant to supplementary UV-B radiation. The rcd1-1 mutant exhibits less accumulation<br/><br>
of TT dimers, increased hypocotyl growth inhibition and higher accumulation of flavonoids under<br/><br>
supplemental UV-B radiation. Moreover, the expression of HY5 (ELONGATED HYPOCOTYL5) is<br/><br>
increased in the mutant after UV-B treatment. Gene expression downstream of UV-B signaling reveals<br/><br>
that COP1 (CONSTITUTIVELY PHOTOMORPHOGENIC1)-regulated genes have an elevated<br/><br>
expression in rcd1-1 mutant under UV-B radiation, while expression of UVR8 (UV RESISTANCE<br/><br>
LOCUS 8)-regulated and HY5-independent genes are not changed. Interestingly, the expression of<br/><br>
RCD1 genes is not significantly changed by UV-B radiation. Previous study has shown that STO<br/><br>
protein is interacting with RCD1 in vitro. Here, we found the mRNA level of STO (SALT<br/><br>
TOLERANCE) is greatly increased in rcd1-1 mutant after UV-B radiation. However, UV-B-induced<br/><br>
HY5 and CHS expression is partially inhibited in sto mutant. Based on the above results, it is deduced<br/><br>
that the RCD1, working together with STO, is involved in Arabidopsis UV-B signaling.},
  author       = {Jiang, Lei and Wang, Yan and Björn, Lars Olof and Li, Shaoshan},
  issn         = {1474-9092},
  keyword      = {UV-B,ultraviolet radiation,signaling,Arabidopis,rcd1},
  language     = {eng},
  pages        = {838--846},
  publisher    = {Royal Society of Chemistry},
  series       = {Photochemical and Photobiological Sciences},
  title        = {Arabidopsis RADICAL-INDUCED CELL DEATH1 is involved in UV-B signaling.},
  url          = {http://dx.doi.org/10.1039/b901187k},
  volume       = {8},
  year         = {2009},
}