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Genetic Regulation of Bone Traits Is Influenced by Sex and Reciprocal Cross in F-2 Progeny From GK and F344 Rats

Lagerholm, Sofia LU ; Li, Luo-Sheng; Jiao, Hong; Park, Hee-Bok LU ; Ohlsson, Claes; Åkesson, Kristina LU and Luthman, Holger LU (2009) In Journal of Bone and Mineral Research 24(6). p.1066-1074
Abstract
A genome-wide linkage analysis to identify quantitative trait loci (QTLs) for bone phenotypes was performed in an F-2 intercross of inbred spontaneously type 2 diabetic GK and normoglycemic F344 rats (108 males and 98 females). The aim of the study was to locate genome regions with candidate genes affecting trabecular and cortical bone and to investigate the effects of sex and reciprocal cross. pQCT was used to determine tibia] bone phenotypes in the F2 rats, comprising reciprocal crosses with divergent mitochondrial (mt) DNA. Sex and reciprocal cross-separated QTL analyses were performed followed by assessment of specific interactions. Four genome-wide significant QTLs linked to either cortical vBMD, tibia length, body length, or... (More)
A genome-wide linkage analysis to identify quantitative trait loci (QTLs) for bone phenotypes was performed in an F-2 intercross of inbred spontaneously type 2 diabetic GK and normoglycemic F344 rats (108 males and 98 females). The aim of the study was to locate genome regions with candidate genes affecting trabecular and cortical bone and to investigate the effects of sex and reciprocal cross. pQCT was used to determine tibia] bone phenotypes in the F2 rats, comprising reciprocal crosses with divergent mitochondrial (mt) DNA. Sex and reciprocal cross-separated QTL analyses were performed followed by assessment of specific interactions. Four genome-wide significant QTLs linked to either cortical vBMD, tibia length, body length, or metaphyseal area were identified in males on chromosomes (chr) 1, 8, and 15. In females, three significant QTLs linked to cortical BMC or metaphyseal total vBMD were identified on chr 1 and 2. Several additional suggestive loci for trabecular and cortical traits were detected in both males and females. Four female-specific QTLs on chr 2, 3, 5, and 10 and four reciprocal cross-specific QTLs on chr 1, 10, and 18 were identified, suggesting that both sex and mt genotype influence the expression of bone phenotypes. J Bone Miner Res 2009;24:1066-1074. Published online on December 29, 2008; doi: 10.1359/JBMR.081252 (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
mitochondria, reciprocal cross, GK rat, quantitative trait loci, pQCT
in
Journal of Bone and Mineral Research
volume
24
issue
6
pages
1066 - 1074
publisher
AMBMR
external identifiers
  • wos:000266358100011
  • scopus:66349107959
ISSN
1523-4681
DOI
10.1359/JBMR.081252
language
English
LU publication?
yes
id
701fa2b5-cff0-4eb1-b850-7210aa8d9641 (old id 1425029)
date added to LUP
2009-07-02 16:42:56
date last changed
2017-01-01 04:36:04
@article{701fa2b5-cff0-4eb1-b850-7210aa8d9641,
  abstract     = {A genome-wide linkage analysis to identify quantitative trait loci (QTLs) for bone phenotypes was performed in an F-2 intercross of inbred spontaneously type 2 diabetic GK and normoglycemic F344 rats (108 males and 98 females). The aim of the study was to locate genome regions with candidate genes affecting trabecular and cortical bone and to investigate the effects of sex and reciprocal cross. pQCT was used to determine tibia] bone phenotypes in the F2 rats, comprising reciprocal crosses with divergent mitochondrial (mt) DNA. Sex and reciprocal cross-separated QTL analyses were performed followed by assessment of specific interactions. Four genome-wide significant QTLs linked to either cortical vBMD, tibia length, body length, or metaphyseal area were identified in males on chromosomes (chr) 1, 8, and 15. In females, three significant QTLs linked to cortical BMC or metaphyseal total vBMD were identified on chr 1 and 2. Several additional suggestive loci for trabecular and cortical traits were detected in both males and females. Four female-specific QTLs on chr 2, 3, 5, and 10 and four reciprocal cross-specific QTLs on chr 1, 10, and 18 were identified, suggesting that both sex and mt genotype influence the expression of bone phenotypes. J Bone Miner Res 2009;24:1066-1074. Published online on December 29, 2008; doi: 10.1359/JBMR.081252},
  author       = {Lagerholm, Sofia and Li, Luo-Sheng and Jiao, Hong and Park, Hee-Bok and Ohlsson, Claes and Åkesson, Kristina and Luthman, Holger},
  issn         = {1523-4681},
  keyword      = {mitochondria,reciprocal cross,GK rat,quantitative trait loci,pQCT},
  language     = {eng},
  number       = {6},
  pages        = {1066--1074},
  publisher    = {AMBMR},
  series       = {Journal of Bone and Mineral Research},
  title        = {Genetic Regulation of Bone Traits Is Influenced by Sex and Reciprocal Cross in F-2 Progeny From GK and F344 Rats},
  url          = {http://dx.doi.org/10.1359/JBMR.081252},
  volume       = {24},
  year         = {2009},
}