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Using microdispensing to manufacture a customized cell dish for microbeam irradiation of single, living cells

Nilsson, E.J.C.; Gram, Magnus LU ; Nilsson, J.; Pallon, Jan LU ; Masternak, Anna LU ; Paczesny, J.; Arteaga, Natalia LU ; Elfman, Mikael LU ; Kristiansson, Per LU and Nilsson, Charlotta LU , et al. (2009) In Nuclear Instruments & Methods in Physics Research. Section B: Beam Interactions with Materials and Atoms 267(7). p.1199-1205
Abstract
In this paper is described the preparation of patterned cell dishes to be used in studies of low dose irradiation effects on living cells. Using a droplet microdispenser, an 8 mu m thick polypropylene cell substrate, to which cells do not naturally adhere, was coated in a matrix pattern with the cell adhesive mussel protein Cell-Tak. Cells were shown to adhere and grow on the protein-coated spots, but not on the uncoated parts, providing for guided cell growth. Cultivation of isolated cell colonies provides an opportunity to study how low doses of ionizing radiation affect neighbouring un-irradiated cell colonies. (C) 2009 Elsevier B.V. All rights reserved.
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keywords
irradiation, Microbeam, Patterned cell dish, Microdispensing, Cell substrate, Bystander effect, HepG2 cells
in
Nuclear Instruments & Methods in Physics Research. Section B: Beam Interactions with Materials and Atoms
volume
267
issue
7
pages
1199 - 1205
publisher
Elsevier
external identifiers
  • wos:000266155000028
  • scopus:64349101894
ISSN
0168-583X
DOI
10.1016/j.nimb.2009.02.051
language
English
LU publication?
yes
id
a2347590-91df-4dc2-bd7b-d82feb7ff6ce (old id 1425573)
date added to LUP
2009-07-01 14:02:59
date last changed
2017-01-01 05:22:05
@article{a2347590-91df-4dc2-bd7b-d82feb7ff6ce,
  abstract     = {In this paper is described the preparation of patterned cell dishes to be used in studies of low dose irradiation effects on living cells. Using a droplet microdispenser, an 8 mu m thick polypropylene cell substrate, to which cells do not naturally adhere, was coated in a matrix pattern with the cell adhesive mussel protein Cell-Tak. Cells were shown to adhere and grow on the protein-coated spots, but not on the uncoated parts, providing for guided cell growth. Cultivation of isolated cell colonies provides an opportunity to study how low doses of ionizing radiation affect neighbouring un-irradiated cell colonies. (C) 2009 Elsevier B.V. All rights reserved.},
  author       = {Nilsson, E.J.C. and Gram, Magnus and Nilsson, J. and Pallon, Jan and Masternak, Anna and Paczesny, J. and Arteaga, Natalia and Elfman, Mikael and Kristiansson, Per and Nilsson, Charlotta and Åkerström, Bo},
  issn         = {0168-583X},
  keyword      = {irradiation,Microbeam,Patterned cell dish,Microdispensing,Cell substrate,Bystander effect,HepG2 cells},
  language     = {eng},
  number       = {7},
  pages        = {1199--1205},
  publisher    = {Elsevier},
  series       = {Nuclear Instruments & Methods in Physics Research. Section B: Beam Interactions with Materials and Atoms},
  title        = {Using microdispensing to manufacture a customized cell dish for microbeam irradiation of single, living cells},
  url          = {http://dx.doi.org/10.1016/j.nimb.2009.02.051},
  volume       = {267},
  year         = {2009},
}