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Proteome analysis of the xylose-fermenting mutant yeast strain TMB 3400.

Karhumaa, Kaisa LU ; Påhlman, Anna-Karin; Hahn-Hägerdal, Bärbel LU ; Levander, Fredrik LU and Gorwa-Grauslund, Marie-Francoise LU (2009) In Yeast 26(7). p.371-382
Abstract
Xylose fermentation in yeast has been a target of research for years, yet not all the factors that may affect xylose fermentation perfomance of yeast strains are known. In this study, the mutant S. cerevisiae strain TMB 3400, which has good xylose fermentation properties, was compared with its parental strain to examine the factors behind the improved xylose utilization at protein level. The proteome of the parental and the mutant strains were characterized by difference in gel electrophoresis (DiGE) to quantitatively identify proteins that are expressed at altered levels in the mutant. The most significant changes detected by proteome analysis were the 6-10-fold increased levels of xylose reductase, xylitol dehydrogenase and transketolase... (More)
Xylose fermentation in yeast has been a target of research for years, yet not all the factors that may affect xylose fermentation perfomance of yeast strains are known. In this study, the mutant S. cerevisiae strain TMB 3400, which has good xylose fermentation properties, was compared with its parental strain to examine the factors behind the improved xylose utilization at protein level. The proteome of the parental and the mutant strains were characterized by difference in gel electrophoresis (DiGE) to quantitatively identify proteins that are expressed at altered levels in the mutant. The most significant changes detected by proteome analysis were the 6-10-fold increased levels of xylose reductase, xylitol dehydrogenase and transketolase (Tkl1) in the mutant, which is in accordance with previous knowledge about xylose metabolism in yeast. The level of acetaldehyde dehydrogenase (Ald6) was also significantly increased. In addition, several proteins homologous to proteins from yeast species other than S. cerevisiae were identified in both strains, demonstrating the genetic heterogeneity of industrial yeast strains. The results were also compared with a previously reported transcription analysis performed with identical experimental set-up; however, very little correlation between the two datasets was observed. The results of the proteome analysis were in good agreement with a parallel study in which rationally designed overexpression of XR, XDH and the non-oxidative pentose phosphate pathway resulted in similar improvement in xylose utilization, which demonstrates the usefulness of proteome analysis for the identification of target genes for further metabolic engineering strategies in industrial yeast strains. Copyright (c) 2009 John Wiley & Sons, Ltd. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Yeast
volume
26
issue
7
pages
371 - 382
publisher
John Wiley & Sons
external identifiers
  • wos:000268035200002
  • pmid:19504622
  • scopus:68349115041
ISSN
1097-0061
DOI
10.1002/yea.1673
language
English
LU publication?
yes
id
ba3c647e-c25a-4f57-a050-4c2a4264f6c8 (old id 1434421)
date added to LUP
2009-07-01 16:04:22
date last changed
2017-10-08 04:01:29
@article{ba3c647e-c25a-4f57-a050-4c2a4264f6c8,
  abstract     = {Xylose fermentation in yeast has been a target of research for years, yet not all the factors that may affect xylose fermentation perfomance of yeast strains are known. In this study, the mutant S. cerevisiae strain TMB 3400, which has good xylose fermentation properties, was compared with its parental strain to examine the factors behind the improved xylose utilization at protein level. The proteome of the parental and the mutant strains were characterized by difference in gel electrophoresis (DiGE) to quantitatively identify proteins that are expressed at altered levels in the mutant. The most significant changes detected by proteome analysis were the 6-10-fold increased levels of xylose reductase, xylitol dehydrogenase and transketolase (Tkl1) in the mutant, which is in accordance with previous knowledge about xylose metabolism in yeast. The level of acetaldehyde dehydrogenase (Ald6) was also significantly increased. In addition, several proteins homologous to proteins from yeast species other than S. cerevisiae were identified in both strains, demonstrating the genetic heterogeneity of industrial yeast strains. The results were also compared with a previously reported transcription analysis performed with identical experimental set-up; however, very little correlation between the two datasets was observed. The results of the proteome analysis were in good agreement with a parallel study in which rationally designed overexpression of XR, XDH and the non-oxidative pentose phosphate pathway resulted in similar improvement in xylose utilization, which demonstrates the usefulness of proteome analysis for the identification of target genes for further metabolic engineering strategies in industrial yeast strains. Copyright (c) 2009 John Wiley & Sons, Ltd.},
  author       = {Karhumaa, Kaisa and Påhlman, Anna-Karin and Hahn-Hägerdal, Bärbel and Levander, Fredrik and Gorwa-Grauslund, Marie-Francoise},
  issn         = {1097-0061},
  language     = {eng},
  number       = {7},
  pages        = {371--382},
  publisher    = {John Wiley & Sons},
  series       = {Yeast},
  title        = {Proteome analysis of the xylose-fermenting mutant yeast strain TMB 3400.},
  url          = {http://dx.doi.org/10.1002/yea.1673},
  volume       = {26},
  year         = {2009},
}