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Human Islet Amyloid Polypeptide Transgenic Mice: In Vivo and Ex Vivo Models for the Role of hIAPP in Type 2 Diabetes Mellitus

Hoppener, J. W. M. ; Jacobs, H. M. ; Wierup, N. ; Sotthewes, G. ; Sprong, M. ; de Vos, P. ; Berger, R. ; Sundler, Frank LU and Ahrén, Bo LU (2008) In Experimental Diabetes Research
Abstract
Human islet amyloid polypeptide (hIAPP), a pancreatic islet protein of 37 amino acids, is the main component of islet amyloid, seen at autopsy in patients with type 2 diabetes mellitus (DM2). To investigate the roles of hIAPP and islet amyloid in DM2, we generated transgenic mice expressing hIAPP in their islet beta cells. In this study, we found that after a long-term, high-fat diet challenge islet amyloid was observed in only 4 of 19 hIAPP transgenic mice. hIAPP transgenic females exhibited severe glucose intolerance, which was associated with a downregulation of GLUT-2 mRNA expression. In isolated islets from hIAPP males cultured for 3 weeks on high-glucose medium, the percentage of amyloid containing islets increased from 5.5% to 70%.... (More)
Human islet amyloid polypeptide (hIAPP), a pancreatic islet protein of 37 amino acids, is the main component of islet amyloid, seen at autopsy in patients with type 2 diabetes mellitus (DM2). To investigate the roles of hIAPP and islet amyloid in DM2, we generated transgenic mice expressing hIAPP in their islet beta cells. In this study, we found that after a long-term, high-fat diet challenge islet amyloid was observed in only 4 of 19 hIAPP transgenic mice. hIAPP transgenic females exhibited severe glucose intolerance, which was associated with a downregulation of GLUT-2 mRNA expression. In isolated islets from hIAPP males cultured for 3 weeks on high-glucose medium, the percentage of amyloid containing islets increased from 5.5% to 70%. This ex vivo system will allow a more rapid, convenient, and specific study of factors influencing islet amyloidosis as well as of therapeutic strategies to interfere with this pathological process. Copyright (C) 2008 J. W. M. Hoppener et al. (Less)
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publishing date
type
Contribution to journal
publication status
published
subject
in
Experimental Diabetes Research
article number
697035
publisher
Hindawi Limited
external identifiers
  • wos:000267194700001
  • scopus:44649119876
ISSN
1687-5214
DOI
10.1155/2008/697035
language
English
LU publication?
yes
additional info
The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Neuroendocrine Cell Biology (013212008), Medicine (Lund) (013230025)
id
cf737454-377c-4275-a323-9699dbe4bda8 (old id 1440493)
date added to LUP
2016-04-01 11:58:48
date last changed
2024-10-08 17:05:12
@article{cf737454-377c-4275-a323-9699dbe4bda8,
  abstract     = {{Human islet amyloid polypeptide (hIAPP), a pancreatic islet protein of 37 amino acids, is the main component of islet amyloid, seen at autopsy in patients with type 2 diabetes mellitus (DM2). To investigate the roles of hIAPP and islet amyloid in DM2, we generated transgenic mice expressing hIAPP in their islet beta cells. In this study, we found that after a long-term, high-fat diet challenge islet amyloid was observed in only 4 of 19 hIAPP transgenic mice. hIAPP transgenic females exhibited severe glucose intolerance, which was associated with a downregulation of GLUT-2 mRNA expression. In isolated islets from hIAPP males cultured for 3 weeks on high-glucose medium, the percentage of amyloid containing islets increased from 5.5% to 70%. This ex vivo system will allow a more rapid, convenient, and specific study of factors influencing islet amyloidosis as well as of therapeutic strategies to interfere with this pathological process. Copyright (C) 2008 J. W. M. Hoppener et al.}},
  author       = {{Hoppener, J. W. M. and Jacobs, H. M. and Wierup, N. and Sotthewes, G. and Sprong, M. and de Vos, P. and Berger, R. and Sundler, Frank and Ahrén, Bo}},
  issn         = {{1687-5214}},
  language     = {{eng}},
  publisher    = {{Hindawi Limited}},
  series       = {{Experimental Diabetes Research}},
  title        = {{Human Islet Amyloid Polypeptide Transgenic Mice: In Vivo and Ex Vivo Models for the Role of hIAPP in Type 2 Diabetes Mellitus}},
  url          = {{http://dx.doi.org/10.1155/2008/697035}},
  doi          = {{10.1155/2008/697035}},
  year         = {{2008}},
}