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The oxygen level determines the fermentation pattern in Kluyveromyces lactis

Merico, Annamaria; Galafassi, Silvia; Piskur, Jure LU and Compagno, Concetta (2009) In FEMS Yeast Research 9(5). p.749-756
Abstract
Yeasts belonging to the lineage that underwent whole-genome duplication (WGD) possess a good fermentative potential and can proliferate in the absence of oxygen. In this study, we analyzed the pre-WGD yeast Kluyveromyces lactis and its ability to grow under oxygen-limited conditions. Under these conditions, K. lactis starts to increase the glucose metabolism and accumulates ethanol and glycerol. However, under more limited conditions, the fermentative metabolism decreases, causing a slow growth rate. In contrast, Saccharomyces cerevisiae and Saccharomyces kluyveri in anaerobiosis exhibit almost the same growth rate as in aerobiosis. In this work, we showed that in K. lactis, under oxygen-limited conditions, a decreased expression of RAG1... (More)
Yeasts belonging to the lineage that underwent whole-genome duplication (WGD) possess a good fermentative potential and can proliferate in the absence of oxygen. In this study, we analyzed the pre-WGD yeast Kluyveromyces lactis and its ability to grow under oxygen-limited conditions. Under these conditions, K. lactis starts to increase the glucose metabolism and accumulates ethanol and glycerol. However, under more limited conditions, the fermentative metabolism decreases, causing a slow growth rate. In contrast, Saccharomyces cerevisiae and Saccharomyces kluyveri in anaerobiosis exhibit almost the same growth rate as in aerobiosis. In this work, we showed that in K. lactis, under oxygen-limited conditions, a decreased expression of RAG1 occurred. The activity of glucose-6-phosphate dehydrogenase also decreased, likely causing a reduced flux in the pentose phosphate pathway. Comparison of related and characterized yeasts suggests that the behavior observed in K. lactis could reflect the lack of an efficient mechanism to maintain a high glycolytic flux and to balance the redox homeostasis under hypoxic conditions. This could be a consequence of a recent specialization of K. lactis toward living in a niche where the ethanol accumulation at high oxygen concentrations and the ability to survive at a low oxygen concentration do not represent an advantage. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
redox, hypoxia, ethanol, Kluyveromyces lactis, fermentation
in
FEMS Yeast Research
volume
9
issue
5
pages
749 - 756
publisher
Elsevier
external identifiers
  • wos:000267751800008
  • scopus:67651148300
ISSN
1567-1364
DOI
10.1111/j.1567-1364.2009.00528.x
language
English
LU publication?
yes
id
4c0fdb3d-c8d9-41c4-97ea-002f03e6b333 (old id 1462715)
date added to LUP
2009-08-19 09:23:50
date last changed
2017-10-22 03:39:00
@article{4c0fdb3d-c8d9-41c4-97ea-002f03e6b333,
  abstract     = {Yeasts belonging to the lineage that underwent whole-genome duplication (WGD) possess a good fermentative potential and can proliferate in the absence of oxygen. In this study, we analyzed the pre-WGD yeast Kluyveromyces lactis and its ability to grow under oxygen-limited conditions. Under these conditions, K. lactis starts to increase the glucose metabolism and accumulates ethanol and glycerol. However, under more limited conditions, the fermentative metabolism decreases, causing a slow growth rate. In contrast, Saccharomyces cerevisiae and Saccharomyces kluyveri in anaerobiosis exhibit almost the same growth rate as in aerobiosis. In this work, we showed that in K. lactis, under oxygen-limited conditions, a decreased expression of RAG1 occurred. The activity of glucose-6-phosphate dehydrogenase also decreased, likely causing a reduced flux in the pentose phosphate pathway. Comparison of related and characterized yeasts suggests that the behavior observed in K. lactis could reflect the lack of an efficient mechanism to maintain a high glycolytic flux and to balance the redox homeostasis under hypoxic conditions. This could be a consequence of a recent specialization of K. lactis toward living in a niche where the ethanol accumulation at high oxygen concentrations and the ability to survive at a low oxygen concentration do not represent an advantage.},
  author       = {Merico, Annamaria and Galafassi, Silvia and Piskur, Jure and Compagno, Concetta},
  issn         = {1567-1364},
  keyword      = {redox,hypoxia,ethanol,Kluyveromyces lactis,fermentation},
  language     = {eng},
  number       = {5},
  pages        = {749--756},
  publisher    = {Elsevier},
  series       = {FEMS Yeast Research},
  title        = {The oxygen level determines the fermentation pattern in Kluyveromyces lactis},
  url          = {http://dx.doi.org/10.1111/j.1567-1364.2009.00528.x},
  volume       = {9},
  year         = {2009},
}