Development of a high performance anion exchange chromatography analysis for mapping of oligosaccharides
(2009) In Journal of Chromatography. B 877(20-21). p.1827-1832- Abstract
- In the present study a HPAEC-PAD method is described that was developed for monitoring the consistency of N-glycosylation during the production and purification of recombinant proteins and monoclonal antibodies. The method successfully separated 18 neutral and sialylated oligosaccharides. Results obtained were compared with MALDI-TOF MS and it was shown that both methods gave similar results. in addition, a method validation was performed showing that the HPAEC-PAD analysis was well suited for the mapping and characterization of oligosaccharides. The method was found to be robust and additionally the precision was significantly better compared to the MALDI-TOF MS method. (C) 2009 Elsevier B.V. All rights reserved.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1463105
- author
- Grey, Carl LU ; Edebrink, Per ; Krook, Maria and Jacobsson, Sven P.
- organization
- publishing date
- 2009
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Monoclonal antibodies, Glycoprotein, N-glycosylation, Oligosaccharides, HPAEC-PAD, MALDI-TOF MS
- in
- Journal of Chromatography. B
- volume
- 877
- issue
- 20-21
- pages
- 1827 - 1832
- publisher
- Elsevier
- external identifiers
-
- wos:000267453800006
- scopus:67249087592
- pmid:19482525
- ISSN
- 1873-376X
- DOI
- 10.1016/j.jchromb.2009.05.003
- language
- English
- LU publication?
- yes
- id
- 73a71c15-d4bf-4762-af0e-d1f7dc18874e (old id 1463105)
- date added to LUP
- 2016-04-01 12:07:33
- date last changed
- 2024-10-08 22:35:23
@article{73a71c15-d4bf-4762-af0e-d1f7dc18874e, abstract = {{In the present study a HPAEC-PAD method is described that was developed for monitoring the consistency of N-glycosylation during the production and purification of recombinant proteins and monoclonal antibodies. The method successfully separated 18 neutral and sialylated oligosaccharides. Results obtained were compared with MALDI-TOF MS and it was shown that both methods gave similar results. in addition, a method validation was performed showing that the HPAEC-PAD analysis was well suited for the mapping and characterization of oligosaccharides. The method was found to be robust and additionally the precision was significantly better compared to the MALDI-TOF MS method. (C) 2009 Elsevier B.V. All rights reserved.}}, author = {{Grey, Carl and Edebrink, Per and Krook, Maria and Jacobsson, Sven P.}}, issn = {{1873-376X}}, keywords = {{Monoclonal antibodies; Glycoprotein; N-glycosylation; Oligosaccharides; HPAEC-PAD; MALDI-TOF MS}}, language = {{eng}}, number = {{20-21}}, pages = {{1827--1832}}, publisher = {{Elsevier}}, series = {{Journal of Chromatography. B}}, title = {{Development of a high performance anion exchange chromatography analysis for mapping of oligosaccharides}}, url = {{http://dx.doi.org/10.1016/j.jchromb.2009.05.003}}, doi = {{10.1016/j.jchromb.2009.05.003}}, volume = {{877}}, year = {{2009}}, }