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Development of a high performance anion exchange chromatography analysis for mapping of oligosaccharides

Grey, Carl LU ; Edebrink, Per; Krook, Maria and Jacobsson, Sven P. (2009) In Journal of Chromatography. B 877(20-21). p.1827-1832
Abstract
In the present study a HPAEC-PAD method is described that was developed for monitoring the consistency of N-glycosylation during the production and purification of recombinant proteins and monoclonal antibodies. The method successfully separated 18 neutral and sialylated oligosaccharides. Results obtained were compared with MALDI-TOF MS and it was shown that both methods gave similar results. in addition, a method validation was performed showing that the HPAEC-PAD analysis was well suited for the mapping and characterization of oligosaccharides. The method was found to be robust and additionally the precision was significantly better compared to the MALDI-TOF MS method. (C) 2009 Elsevier B.V. All rights reserved.
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Monoclonal antibodies, Glycoprotein, N-glycosylation, Oligosaccharides, HPAEC-PAD, MALDI-TOF MS
in
Journal of Chromatography. B
volume
877
issue
20-21
pages
1827 - 1832
publisher
Elsevier
external identifiers
  • wos:000267453800006
  • scopus:67249087592
ISSN
1873-376X
DOI
10.1016/j.jchromb.2009.05.003
language
English
LU publication?
yes
id
73a71c15-d4bf-4762-af0e-d1f7dc18874e (old id 1463105)
date added to LUP
2009-08-18 14:39:16
date last changed
2017-09-17 05:00:13
@article{73a71c15-d4bf-4762-af0e-d1f7dc18874e,
  abstract     = {In the present study a HPAEC-PAD method is described that was developed for monitoring the consistency of N-glycosylation during the production and purification of recombinant proteins and monoclonal antibodies. The method successfully separated 18 neutral and sialylated oligosaccharides. Results obtained were compared with MALDI-TOF MS and it was shown that both methods gave similar results. in addition, a method validation was performed showing that the HPAEC-PAD analysis was well suited for the mapping and characterization of oligosaccharides. The method was found to be robust and additionally the precision was significantly better compared to the MALDI-TOF MS method. (C) 2009 Elsevier B.V. All rights reserved.},
  author       = {Grey, Carl and Edebrink, Per and Krook, Maria and Jacobsson, Sven P.},
  issn         = {1873-376X},
  keyword      = {Monoclonal antibodies,Glycoprotein,N-glycosylation,Oligosaccharides,HPAEC-PAD,MALDI-TOF MS},
  language     = {eng},
  number       = {20-21},
  pages        = {1827--1832},
  publisher    = {Elsevier},
  series       = {Journal of Chromatography. B},
  title        = {Development of a high performance anion exchange chromatography analysis for mapping of oligosaccharides},
  url          = {http://dx.doi.org/10.1016/j.jchromb.2009.05.003},
  volume       = {877},
  year         = {2009},
}