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DivIVA uses an N-terminal conserved region and two coiled-coil domains to localize and sustain the polar growth in Corynebacterium glutamicum

Letek, Michal; Fiuza, Maria; Ordonez, Efren; Villadangos, Almudena F.; Flärdh, Klas LU ; Mateos, Luis M. and Gil, Jose A. (2009) In FEMS Microbiology Letters 297(1). p.110-116
Abstract
Corynebacterium glutamicum is a rod-shaped actinomycete with a distinct model of peptidoglycan synthesis during cell elongation, which takes place at the cell poles and is sustained by the essential protein DivIVA(CG) (C. glutamicum DivIVA). This protein contains a short conserved N-terminal domain and two coiled-coil regions: CC1 and CC2. Domain deletions and chimeric versions of DivIVA were used to functionally characterize the three domains, and all three were found to be essential for proper DivIVA(CG) function. However, in the presence of the N-terminal domain from DivIVA(CG), either of the two coiled-coil domains of DivIVA(CG) could be replaced by the equivalent coiled-coil domain of Bacillus subtilis DivIVA (DivIVA(BS)) without... (More)
Corynebacterium glutamicum is a rod-shaped actinomycete with a distinct model of peptidoglycan synthesis during cell elongation, which takes place at the cell poles and is sustained by the essential protein DivIVA(CG) (C. glutamicum DivIVA). This protein contains a short conserved N-terminal domain and two coiled-coil regions: CC1 and CC2. Domain deletions and chimeric versions of DivIVA were used to functionally characterize the three domains, and all three were found to be essential for proper DivIVA(CG) function. However, in the presence of the N-terminal domain from DivIVA(CG), either of the two coiled-coil domains of DivIVA(CG) could be replaced by the equivalent coiled-coil domain of Bacillus subtilis DivIVA (DivIVA(BS)) without affecting the function of the original DivIVA(CG), and more than one domain had to be exchanged to lose function. Although no single domain was sufficient for subcellular localization or function, CC1 was mainly implicated in stimulating polar growth and CC2 in targeting to DivIVA(CG) assemblies at the cell poles in C. glutamicum. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
swapping domain, polar growth, DivIVA, Corynebacterium, cell division, two-hybrid system
in
FEMS Microbiology Letters
volume
297
issue
1
pages
110 - 116
publisher
Elsevier
external identifiers
  • wos:000267697700016
  • scopus:67650721277
ISSN
1574-6968
DOI
10.1111/j.1574-6968.2009.01679.x
language
English
LU publication?
yes
id
19b6014f-080d-458f-8887-b5a8d510204a (old id 1463240)
date added to LUP
2009-08-18 13:40:45
date last changed
2017-04-02 03:40:37
@article{19b6014f-080d-458f-8887-b5a8d510204a,
  abstract     = {Corynebacterium glutamicum is a rod-shaped actinomycete with a distinct model of peptidoglycan synthesis during cell elongation, which takes place at the cell poles and is sustained by the essential protein DivIVA(CG) (C. glutamicum DivIVA). This protein contains a short conserved N-terminal domain and two coiled-coil regions: CC1 and CC2. Domain deletions and chimeric versions of DivIVA were used to functionally characterize the three domains, and all three were found to be essential for proper DivIVA(CG) function. However, in the presence of the N-terminal domain from DivIVA(CG), either of the two coiled-coil domains of DivIVA(CG) could be replaced by the equivalent coiled-coil domain of Bacillus subtilis DivIVA (DivIVA(BS)) without affecting the function of the original DivIVA(CG), and more than one domain had to be exchanged to lose function. Although no single domain was sufficient for subcellular localization or function, CC1 was mainly implicated in stimulating polar growth and CC2 in targeting to DivIVA(CG) assemblies at the cell poles in C. glutamicum.},
  author       = {Letek, Michal and Fiuza, Maria and Ordonez, Efren and Villadangos, Almudena F. and Flärdh, Klas and Mateos, Luis M. and Gil, Jose A.},
  issn         = {1574-6968},
  keyword      = {swapping domain,polar growth,DivIVA,Corynebacterium,cell division,two-hybrid system},
  language     = {eng},
  number       = {1},
  pages        = {110--116},
  publisher    = {Elsevier},
  series       = {FEMS Microbiology Letters},
  title        = {DivIVA uses an N-terminal conserved region and two coiled-coil domains to localize and sustain the polar growth in Corynebacterium glutamicum},
  url          = {http://dx.doi.org/10.1111/j.1574-6968.2009.01679.x},
  volume       = {297},
  year         = {2009},
}