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Phosphorus effects on metabolic processes in monoxenic arbuscular mycorrhiza cultures

Olsson, Pål Axel LU ; van Aarle, Ingrid LU ; Allaway, W G; Ashford, A E and Rouhier, Hervé LU (2002) In Plant Physiology 130(3). p.1162-1171
Abstract
The influence of external phosphorus (P) on carbon (C) allocation and metabolism as well as processes related to P metabolism was studied in monoxenic arbuscular mycorrhiza cultures of carrot (Daucus carota). Fungal hyphae of Glomus intraradices proliferated from the solid minimal medium containing the colonized roots into C-free liquid minimal medium with different P treatments. The fungus formed around three times higher biomass in P-free liquid medium than in medium with 2.5 mM inorganic P (high-P). Mycelium in the second experiment was harvested at an earlier growth stag to study metabolic processes when the mycelium was actively growing. P treatment influenced the root P content and [C-13]glucose administered to the roots 7 d before... (More)
The influence of external phosphorus (P) on carbon (C) allocation and metabolism as well as processes related to P metabolism was studied in monoxenic arbuscular mycorrhiza cultures of carrot (Daucus carota). Fungal hyphae of Glomus intraradices proliferated from the solid minimal medium containing the colonized roots into C-free liquid minimal medium with different P treatments. The fungus formed around three times higher biomass in P-free liquid medium than in medium with 2.5 mM inorganic P (high-P). Mycelium in the second experiment was harvested at an earlier growth stag to study metabolic processes when the mycelium was actively growing. P treatment influenced the root P content and [C-13]glucose administered to the roots 7 d before harvest gave a negative correlation between root P content and C-13 enrichment in arbuscular mycorrhiza fungal storage lipids in the extraradical hyphae. Eighteen percent of the enriched C-13 in extraradical hyphae was recovered in the fatty acid 16:1omega5 from neutral lipids. Polyphosphate accumulated in hyphae even in P-free medium. No influence of P treatment on fungal acid phosphatase activity was observed, whereas the proportion of alkaline-phosphatase-active hyphae was highest in high-P medium. We demonstrated the presence of a motile tubular vacuolar system in G. intraradices. This system was rarely seen in hyphae subjected to the highest P treatment. We concluded that the direct responses of the extraradical hyphae to the P concentration in the medium are limited. The effects found in hyphae seemed instead to be related to increased availability of P to the host root. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Plant Physiology
volume
130
issue
3
pages
1162 - 1171
publisher
American Society of Plant Biologists
external identifiers
  • pmid:12427983
  • wos:000179329400010
  • scopus:0036852160
ISSN
1532-2548
DOI
10.1104/pp.009639
language
English
LU publication?
yes
id
e1a650cb-1e3f-4b53-80ba-374ae19bda50 (old id 146885)
date added to LUP
2007-06-29 14:07:00
date last changed
2017-12-10 03:52:51
@article{e1a650cb-1e3f-4b53-80ba-374ae19bda50,
  abstract     = {The influence of external phosphorus (P) on carbon (C) allocation and metabolism as well as processes related to P metabolism was studied in monoxenic arbuscular mycorrhiza cultures of carrot (Daucus carota). Fungal hyphae of Glomus intraradices proliferated from the solid minimal medium containing the colonized roots into C-free liquid minimal medium with different P treatments. The fungus formed around three times higher biomass in P-free liquid medium than in medium with 2.5 mM inorganic P (high-P). Mycelium in the second experiment was harvested at an earlier growth stag to study metabolic processes when the mycelium was actively growing. P treatment influenced the root P content and [C-13]glucose administered to the roots 7 d before harvest gave a negative correlation between root P content and C-13 enrichment in arbuscular mycorrhiza fungal storage lipids in the extraradical hyphae. Eighteen percent of the enriched C-13 in extraradical hyphae was recovered in the fatty acid 16:1omega5 from neutral lipids. Polyphosphate accumulated in hyphae even in P-free medium. No influence of P treatment on fungal acid phosphatase activity was observed, whereas the proportion of alkaline-phosphatase-active hyphae was highest in high-P medium. We demonstrated the presence of a motile tubular vacuolar system in G. intraradices. This system was rarely seen in hyphae subjected to the highest P treatment. We concluded that the direct responses of the extraradical hyphae to the P concentration in the medium are limited. The effects found in hyphae seemed instead to be related to increased availability of P to the host root.},
  author       = {Olsson, Pål Axel and van Aarle, Ingrid and Allaway, W G and Ashford, A E and Rouhier, Hervé},
  issn         = {1532-2548},
  language     = {eng},
  number       = {3},
  pages        = {1162--1171},
  publisher    = {American Society of Plant Biologists},
  series       = {Plant Physiology},
  title        = {Phosphorus effects on metabolic processes in monoxenic arbuscular mycorrhiza cultures},
  url          = {http://dx.doi.org/10.1104/pp.009639},
  volume       = {130},
  year         = {2002},
}