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Filter-exchange PGSE NMR determination of cell membrane permeability.

Åslund, Ingrid LU ; Nowacka, Agnieszka LU ; Nilsson, Markus LU and Topgaard, Daniel LU (2009) In Journal of Magnetic Resonance 200. p.291-295
Abstract
A new PGSE NMR sequence is introduced for measuring diffusive transport across the plasma membrane of living cells. A "diffusion filter" and a variable mixing time precedes a standard PGSE block for diffusion encoding of the NMR signal. The filter is a PGSE block optimized for selectively removing the magnetization of the extracellular water. With increasing mixing time the intra- and extracellular components approach their equilibrium fractional populations. The rate of exchange can be measured using only a few minutes of instrument time. Water exchange over the plasma membrane of starved yeast cells is studied in the temperature range +5 to +32 degrees C.
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Magnetic Resonance
volume
200
pages
291 - 295
publisher
Elsevier
external identifiers
  • wos:000272260900016
  • pmid:19647458
  • scopus:69949132949
ISSN
1096-0856
DOI
10.1016/j.jmr.2009.07.015
language
English
LU publication?
yes
id
55c69fc1-6eb9-41cc-bb4b-dba3beb1682e (old id 1470185)
date added to LUP
2009-09-08 13:36:33
date last changed
2017-10-22 04:08:36
@article{55c69fc1-6eb9-41cc-bb4b-dba3beb1682e,
  abstract     = {A new PGSE NMR sequence is introduced for measuring diffusive transport across the plasma membrane of living cells. A "diffusion filter" and a variable mixing time precedes a standard PGSE block for diffusion encoding of the NMR signal. The filter is a PGSE block optimized for selectively removing the magnetization of the extracellular water. With increasing mixing time the intra- and extracellular components approach their equilibrium fractional populations. The rate of exchange can be measured using only a few minutes of instrument time. Water exchange over the plasma membrane of starved yeast cells is studied in the temperature range +5 to +32 degrees C.},
  author       = {Åslund, Ingrid and Nowacka, Agnieszka and Nilsson, Markus and Topgaard, Daniel},
  issn         = {1096-0856},
  language     = {eng},
  pages        = {291--295},
  publisher    = {Elsevier},
  series       = {Journal of Magnetic Resonance},
  title        = {Filter-exchange PGSE NMR determination of cell membrane permeability.},
  url          = {http://dx.doi.org/10.1016/j.jmr.2009.07.015},
  volume       = {200},
  year         = {2009},
}