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Low molecular weight heparin stimulates myometrial contractility and cervical remodeling in vitro

Ekman-Ordeberg, Gunvor; Hellgren, Margareta; Åkerud, Anna LU ; Andersson, Eva; Dubicke, Aurelija; Sennstrom, Maria; Bystrom, Birgitta; Tzortzatos, Gerasimos; Gomez, Maria LU and Edlund, Mans, et al. (2009) In Acta Obstetricia et Gynecologica Scandinavica 88(9). p.984-989
Abstract
Objectives. The low molecular weight heparin, Dalteparin, shortens human labor time. The aim of this study was to investigate if the mechanism behind this effect involves myometrial contractility and cervical ripening and if the anticoagulative activity is necessary for its effect. Design. Experimental in vitro study. Setting. Lund University and Karolinska Institute, Sweden. Methods. The effect of low molecular weight heparins with or without anticoagulative properties on myometrial contractility was measured in vitro on smooth muscle strips from biopsies obtained at elective cesarean sections. The effects on cervical ripening were assessed in cervical fibroblasts cultured from explants of cervical biopsies obtained at delivery. Main... (More)
Objectives. The low molecular weight heparin, Dalteparin, shortens human labor time. The aim of this study was to investigate if the mechanism behind this effect involves myometrial contractility and cervical ripening and if the anticoagulative activity is necessary for its effect. Design. Experimental in vitro study. Setting. Lund University and Karolinska Institute, Sweden. Methods. The effect of low molecular weight heparins with or without anticoagulative properties on myometrial contractility was measured in vitro on smooth muscle strips from biopsies obtained at elective cesarean sections. The effects on cervical ripening were assessed in cervical fibroblasts cultured from explants of cervical biopsies obtained at delivery. Main outcome measures. Mean force and number of contractions in uterine smooth muscle strips and interleukin-8 (IL-8) secretion in cervical fibroblasts. Results. Myometrial smooth muscle strips pretreated with low molecular weight heparins showed increased contractile activity compared to untreated smooth muscle strips. Secretion of IL-8 from cultured cervical fibroblasts was significantly increased after treatment with low molecular weight heparin. Both these effects were independent of anticoagulative activity of the low molecular weight heparin. Conclusions. A possible underlying mechanism for the shortened labor time after low molecular weight heparin treatment is enhanced myometrial contractility and an increased IL-8 secretion in cervical fibroblast, mimicking the final cervical ripening in vivo. Our data support the notion that anticoagulant activity is not required to promote labor. (Less)
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published
subject
keywords
Low molecular weight heparin, cervical ripening, myometrial, contractility
in
Acta Obstetricia et Gynecologica Scandinavica
volume
88
issue
9
pages
984 - 989
publisher
Wiley-Blackwell
external identifiers
  • wos:000269094900006
  • scopus:70249098883
ISSN
1600-0412
DOI
10.1080/00016340903176818
language
English
LU publication?
yes
id
d77d80fa-ff45-4f2f-8496-14e0378eaf47 (old id 1477115)
date added to LUP
2009-09-24 08:30:08
date last changed
2017-03-12 03:51:21
@article{d77d80fa-ff45-4f2f-8496-14e0378eaf47,
  abstract     = {Objectives. The low molecular weight heparin, Dalteparin, shortens human labor time. The aim of this study was to investigate if the mechanism behind this effect involves myometrial contractility and cervical ripening and if the anticoagulative activity is necessary for its effect. Design. Experimental in vitro study. Setting. Lund University and Karolinska Institute, Sweden. Methods. The effect of low molecular weight heparins with or without anticoagulative properties on myometrial contractility was measured in vitro on smooth muscle strips from biopsies obtained at elective cesarean sections. The effects on cervical ripening were assessed in cervical fibroblasts cultured from explants of cervical biopsies obtained at delivery. Main outcome measures. Mean force and number of contractions in uterine smooth muscle strips and interleukin-8 (IL-8) secretion in cervical fibroblasts. Results. Myometrial smooth muscle strips pretreated with low molecular weight heparins showed increased contractile activity compared to untreated smooth muscle strips. Secretion of IL-8 from cultured cervical fibroblasts was significantly increased after treatment with low molecular weight heparin. Both these effects were independent of anticoagulative activity of the low molecular weight heparin. Conclusions. A possible underlying mechanism for the shortened labor time after low molecular weight heparin treatment is enhanced myometrial contractility and an increased IL-8 secretion in cervical fibroblast, mimicking the final cervical ripening in vivo. Our data support the notion that anticoagulant activity is not required to promote labor.},
  author       = {Ekman-Ordeberg, Gunvor and Hellgren, Margareta and Åkerud, Anna and Andersson, Eva and Dubicke, Aurelija and Sennstrom, Maria and Bystrom, Birgitta and Tzortzatos, Gerasimos and Gomez, Maria and Edlund, Mans and Lindahl, Ulf and Malmström, Anders},
  issn         = {1600-0412},
  keyword      = {Low molecular weight heparin,cervical ripening,myometrial,contractility},
  language     = {eng},
  number       = {9},
  pages        = {984--989},
  publisher    = {Wiley-Blackwell},
  series       = {Acta Obstetricia et Gynecologica Scandinavica},
  title        = {Low molecular weight heparin stimulates myometrial contractility and cervical remodeling in vitro},
  url          = {http://dx.doi.org/10.1080/00016340903176818},
  volume       = {88},
  year         = {2009},
}