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Calcium increases endocytotic vesicle size and accelerates membrane fission in insulin-secreting INS-1 cells.

MacDonald, Patrick LU ; Eliasson, Lena LU and Rorsman, Patrik LU (2005) In Journal of Cell Science 118(Pt 24). p.5911-5920
Abstract
In many cells, endocytotic membrane retrieval is accelerated by Ca2+. The effect of Ca2+ on single endocytotic vesicles and fission pore kinetics was examined by measuring capacitance and conductance changes in small membrane patches of insulin-secreting INS-1 cells. In intact cells, elevation of Ca2+ by glucose stimulation induced a 1.8-fold increase in membrane internalisation. This surprisingly resulted from an increased unitary capacitance of endocytotic vesicles whereas the frequency of endocytosis was unaltered. This effect of glucose was prevented by inhibition of L- or R-type Ca2+ channels. Extracellular (pipette) Ca2+ was found to regulate endocytotic vesicle capacitance in a bimodal manner. Vesicle capacitance was increased at... (More)
In many cells, endocytotic membrane retrieval is accelerated by Ca2+. The effect of Ca2+ on single endocytotic vesicles and fission pore kinetics was examined by measuring capacitance and conductance changes in small membrane patches of insulin-secreting INS-1 cells. In intact cells, elevation of Ca2+ by glucose stimulation induced a 1.8-fold increase in membrane internalisation. This surprisingly resulted from an increased unitary capacitance of endocytotic vesicles whereas the frequency of endocytosis was unaltered. This effect of glucose was prevented by inhibition of L- or R-type Ca2+ channels. Extracellular (pipette) Ca2+ was found to regulate endocytotic vesicle capacitance in a bimodal manner. Vesicle capacitance was increased at intermediate Ca2+ (2.6 mM), but not at high Ca2+ (10 mM). Similar results were obtained upon direct application of 100 nM and 0.5 mM Ca2+ to the intracellular surface of inside-out excised membrane patches, and in these experiments the increase in vesicle capacitance was prevented by the calcineurin inhibitor deltamethrin. Endocytotic fission pore kinetics were accelerated by Ca2+ in both the intact cells and isolated membrane patches; however, the effect in this case was neither bimodal nor deltamethrin sensitive. Membrane retrieval can therefore be upregulated by a Ca2+-dependent increase in endocytotic vesicle size and acceleration of membrane fission in insulin-secreting INS-1 cells. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Membrane fission, Calcium, Endocytic vesicle, INS-1, Insulin
in
Journal of Cell Science
volume
118
issue
Pt 24
pages
5911 - 5920
publisher
The Company of Biologists Ltd
external identifiers
  • wos:000234811600024
  • scopus:30544441347
ISSN
0021-9533
DOI
10.1242/jcs.02685
language
English
LU publication?
yes
id
03f6f437-8ec6-46e8-b529-64ede6ea42c2 (old id 149053)
alternative location
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=16317049&dopt=Abstract
date added to LUP
2007-07-17 12:25:18
date last changed
2017-10-22 03:43:57
@article{03f6f437-8ec6-46e8-b529-64ede6ea42c2,
  abstract     = {In many cells, endocytotic membrane retrieval is accelerated by Ca2+. The effect of Ca2+ on single endocytotic vesicles and fission pore kinetics was examined by measuring capacitance and conductance changes in small membrane patches of insulin-secreting INS-1 cells. In intact cells, elevation of Ca2+ by glucose stimulation induced a 1.8-fold increase in membrane internalisation. This surprisingly resulted from an increased unitary capacitance of endocytotic vesicles whereas the frequency of endocytosis was unaltered. This effect of glucose was prevented by inhibition of L- or R-type Ca2+ channels. Extracellular (pipette) Ca2+ was found to regulate endocytotic vesicle capacitance in a bimodal manner. Vesicle capacitance was increased at intermediate Ca2+ (2.6 mM), but not at high Ca2+ (10 mM). Similar results were obtained upon direct application of 100 nM and 0.5 mM Ca2+ to the intracellular surface of inside-out excised membrane patches, and in these experiments the increase in vesicle capacitance was prevented by the calcineurin inhibitor deltamethrin. Endocytotic fission pore kinetics were accelerated by Ca2+ in both the intact cells and isolated membrane patches; however, the effect in this case was neither bimodal nor deltamethrin sensitive. Membrane retrieval can therefore be upregulated by a Ca2+-dependent increase in endocytotic vesicle size and acceleration of membrane fission in insulin-secreting INS-1 cells.},
  author       = {MacDonald, Patrick and Eliasson, Lena and Rorsman, Patrik},
  issn         = {0021-9533},
  keyword      = {Membrane fission,Calcium,Endocytic vesicle,INS-1,Insulin},
  language     = {eng},
  number       = {Pt 24},
  pages        = {5911--5920},
  publisher    = {The Company of Biologists Ltd},
  series       = {Journal of Cell Science},
  title        = {Calcium increases endocytotic vesicle size and accelerates membrane fission in insulin-secreting INS-1 cells.},
  url          = {http://dx.doi.org/10.1242/jcs.02685},
  volume       = {118},
  year         = {2005},
}