Calcium increases endocytotic vesicle size and accelerates membrane fission in insulin-secreting INS-1 cells.
MacDonald, Patrick LU ; Eliasson, Lena LU
and Rorsman, Patrik
LU
(2005)
In Journal of Cell Science
118(Pt 24).
p.5911-5920
- Abstract
- In many cells, endocytotic membrane retrieval is accelerated by Ca2+. The effect of Ca2+ on single endocytotic vesicles and fission pore kinetics was examined by measuring capacitance and conductance changes in small membrane patches of insulin-secreting INS-1 cells. In intact cells, elevation of Ca2+ by glucose stimulation induced a 1.8-fold increase in membrane internalisation. This surprisingly resulted from an increased unitary capacitance of endocytotic vesicles whereas the frequency of endocytosis was unaltered. This effect of glucose was prevented by inhibition of L- or R-type Ca2+ channels. Extracellular (pipette) Ca2+ was found to regulate endocytotic vesicle capacitance in a bimodal manner. Vesicle capacitance was increased at... (More)
- In many cells, endocytotic membrane retrieval is accelerated by Ca2+. The effect of Ca2+ on single endocytotic vesicles and fission pore kinetics was examined by measuring capacitance and conductance changes in small membrane patches of insulin-secreting INS-1 cells. In intact cells, elevation of Ca2+ by glucose stimulation induced a 1.8-fold increase in membrane internalisation. This surprisingly resulted from an increased unitary capacitance of endocytotic vesicles whereas the frequency of endocytosis was unaltered. This effect of glucose was prevented by inhibition of L- or R-type Ca2+ channels. Extracellular (pipette) Ca2+ was found to regulate endocytotic vesicle capacitance in a bimodal manner. Vesicle capacitance was increased at intermediate Ca2+ (2.6 mM), but not at high Ca2+ (10 mM). Similar results were obtained upon direct application of 100 nM and 0.5 mM Ca2+ to the intracellular surface of inside-out excised membrane patches, and in these experiments the increase in vesicle capacitance was prevented by the calcineurin inhibitor deltamethrin. Endocytotic fission pore kinetics were accelerated by Ca2+ in both the intact cells and isolated membrane patches; however, the effect in this case was neither bimodal nor deltamethrin sensitive. Membrane retrieval can therefore be upregulated by a Ca2+-dependent increase in endocytotic vesicle size and acceleration of membrane fission in insulin-secreting INS-1 cells. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/149053
- author
- MacDonald, Patrick
LU
; Eliasson, Lena
LU
and Rorsman, Patrik
LU
- organization
- publishing date
- 2005
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Membrane fission, Calcium, Endocytic vesicle, INS-1, Insulin
- in
- Journal of Cell Science
- volume
- 118
- issue
- Pt 24
- pages
- 5911 - 5920
- publisher
- The Company of Biologists Ltd
- external identifiers
-
- wos:000234811600024
- scopus:30544441347
- pmid:16317049
- ISSN
- 0021-9533
- DOI
- 10.1242/jcs.02685
- language
- English
- LU publication?
- yes
- id
- 03f6f437-8ec6-46e8-b529-64ede6ea42c2 (old id 149053)
- alternative location
- http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=16317049&dopt=Abstract
- date added to LUP
- 2016-04-01 12:02:03
- date last changed
- 2022-01-26 21:50:44
@article{03f6f437-8ec6-46e8-b529-64ede6ea42c2,
abstract = {{In many cells, endocytotic membrane retrieval is accelerated by Ca2+. The effect of Ca2+ on single endocytotic vesicles and fission pore kinetics was examined by measuring capacitance and conductance changes in small membrane patches of insulin-secreting INS-1 cells. In intact cells, elevation of Ca2+ by glucose stimulation induced a 1.8-fold increase in membrane internalisation. This surprisingly resulted from an increased unitary capacitance of endocytotic vesicles whereas the frequency of endocytosis was unaltered. This effect of glucose was prevented by inhibition of L- or R-type Ca2+ channels. Extracellular (pipette) Ca2+ was found to regulate endocytotic vesicle capacitance in a bimodal manner. Vesicle capacitance was increased at intermediate Ca2+ (2.6 mM), but not at high Ca2+ (10 mM). Similar results were obtained upon direct application of 100 nM and 0.5 mM Ca2+ to the intracellular surface of inside-out excised membrane patches, and in these experiments the increase in vesicle capacitance was prevented by the calcineurin inhibitor deltamethrin. Endocytotic fission pore kinetics were accelerated by Ca2+ in both the intact cells and isolated membrane patches; however, the effect in this case was neither bimodal nor deltamethrin sensitive. Membrane retrieval can therefore be upregulated by a Ca2+-dependent increase in endocytotic vesicle size and acceleration of membrane fission in insulin-secreting INS-1 cells.}},
author = {{MacDonald, Patrick and Eliasson, Lena and Rorsman, Patrik}},
issn = {{0021-9533}},
keywords = {{Membrane fission; Calcium; Endocytic vesicle; INS-1; Insulin}},
language = {{eng}},
number = {{Pt 24}},
pages = {{5911--5920}},
publisher = {{The Company of Biologists Ltd}},
series = {{Journal of Cell Science}},
title = {{Calcium increases endocytotic vesicle size and accelerates membrane fission in insulin-secreting INS-1 cells.}},
url = {{http://dx.doi.org/10.1242/jcs.02685}},
doi = {{10.1242/jcs.02685}},
volume = {{118}},
year = {{2005}},
}