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Crabtree-negative characteristics of recombinant xylose-utilizing Saccharomyces cerevisiae

Souto Maior, Ana Maria LU ; Runquist, David LU and Hahn-Hägerdal, Bärbel LU (2009) In Journal of Biotechnology 143(2). p.119-123
Abstract
For recombinant xylose-utilizing Saccharomyces cerevisiae, ethanol yield and productivity is substantially lower on xylose than on glucose. In contrast to glucose, xylose is a novel substrate for S. cerevisiae and it is not known how this substrate is recognized on a molecular level. Failure to activate appropriate genes during xylose-utilization has the potential to result in sub-optimal metabolism and decreased substrate uptake. Certain differences in fermentative performance between the two substrates have thus been ascribed to variations in regulatory response. In this study differences in substrate utilization of glucose and xylose was analyzed in the recombinant S. cerevisiae strain TMB3400. Continuous cultures were performed with... (More)
For recombinant xylose-utilizing Saccharomyces cerevisiae, ethanol yield and productivity is substantially lower on xylose than on glucose. In contrast to glucose, xylose is a novel substrate for S. cerevisiae and it is not known how this substrate is recognized on a molecular level. Failure to activate appropriate genes during xylose-utilization has the potential to result in sub-optimal metabolism and decreased substrate uptake. Certain differences in fermentative performance between the two substrates have thus been ascribed to variations in regulatory response. In this study differences in substrate utilization of glucose and xylose was analyzed in the recombinant S. cerevisiae strain TMB3400. Continuous cultures were performed with glucose and xylose under carbon- and nitrogen-limited conditions. Whereas biomass yield and substrate uptake rate were similar during carbon-limited conditions, the metabolic profile was highly substrate dependent under nitrogen-limited conditions. While glycerol production occurred in both cases, ethanol production was only observed for glucose cultures. Addition of acetate and 2-deoxyglucose pulses to a xylose-limited culture was able to stimulate transient overflow metabolism and ethanol production. Application of glucose pulses enhanced xylose uptake rate under restricted co-substrate concentrations. Results are discussed in relation to regulation of sugar metabolism in Crabtree-positive and -negative yeast. (C) 2009 Elsevier B.V. All rights reserved. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Xylose, Recombinant Saccharomyces cerevisiae, Crabtree effect, Glucose signaling, fermentation
in
Journal of Biotechnology
volume
143
issue
2
pages
119 - 123
publisher
Elsevier
external identifiers
  • wos:000269850700006
  • scopus:68349160717
ISSN
1873-4863
DOI
10.1016/j.jbiotec.2009.06.022
language
English
LU publication?
yes
id
6e08bc23-5da6-4737-9e2b-7ced130130dd (old id 1490687)
date added to LUP
2009-10-19 11:54:05
date last changed
2017-12-10 03:50:05
@article{6e08bc23-5da6-4737-9e2b-7ced130130dd,
  abstract     = {For recombinant xylose-utilizing Saccharomyces cerevisiae, ethanol yield and productivity is substantially lower on xylose than on glucose. In contrast to glucose, xylose is a novel substrate for S. cerevisiae and it is not known how this substrate is recognized on a molecular level. Failure to activate appropriate genes during xylose-utilization has the potential to result in sub-optimal metabolism and decreased substrate uptake. Certain differences in fermentative performance between the two substrates have thus been ascribed to variations in regulatory response. In this study differences in substrate utilization of glucose and xylose was analyzed in the recombinant S. cerevisiae strain TMB3400. Continuous cultures were performed with glucose and xylose under carbon- and nitrogen-limited conditions. Whereas biomass yield and substrate uptake rate were similar during carbon-limited conditions, the metabolic profile was highly substrate dependent under nitrogen-limited conditions. While glycerol production occurred in both cases, ethanol production was only observed for glucose cultures. Addition of acetate and 2-deoxyglucose pulses to a xylose-limited culture was able to stimulate transient overflow metabolism and ethanol production. Application of glucose pulses enhanced xylose uptake rate under restricted co-substrate concentrations. Results are discussed in relation to regulation of sugar metabolism in Crabtree-positive and -negative yeast. (C) 2009 Elsevier B.V. All rights reserved.},
  author       = {Souto Maior, Ana Maria and Runquist, David and Hahn-Hägerdal, Bärbel},
  issn         = {1873-4863},
  keyword      = {Xylose,Recombinant Saccharomyces cerevisiae,Crabtree effect,Glucose signaling,fermentation},
  language     = {eng},
  number       = {2},
  pages        = {119--123},
  publisher    = {Elsevier},
  series       = {Journal of Biotechnology},
  title        = {Crabtree-negative characteristics of recombinant xylose-utilizing Saccharomyces cerevisiae},
  url          = {http://dx.doi.org/10.1016/j.jbiotec.2009.06.022},
  volume       = {143},
  year         = {2009},
}