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Diversity of Mhc class I and IIB genes in house sparrows (Passer domesticus)

Bonneaud, C; Sorci, G; Morin, V; Westerdahl, Helena LU ; Zoorob, R and Wittzell, Håkan LU (2004) In Immunogenetics 55(12). p.855-865
Abstract
In order to understand the expression and evolution of host resistance to pathogens, we need to examine the links between genetic variability at the major histocompatibility complex (Mhc), phenotypic expression of the immune response and parasite resistance in natural populations. To do so, we characterized the Mhc class I and IIB genes of house sparrows with the goal of designing a PCR-based genotyping method for the Mhc genes using denaturing gradient gel electrophoresis (DGGE). The incredible success of house sparrows in colonizing habitats worldwide allows us to assess the importance of the variability of Mhc genes in the face of various pathogenic pressures. Isolation and sequencing of Mhc class I and IIB alleles revealed that house... (More)
In order to understand the expression and evolution of host resistance to pathogens, we need to examine the links between genetic variability at the major histocompatibility complex (Mhc), phenotypic expression of the immune response and parasite resistance in natural populations. To do so, we characterized the Mhc class I and IIB genes of house sparrows with the goal of designing a PCR-based genotyping method for the Mhc genes using denaturing gradient gel electrophoresis (DGGE). The incredible success of house sparrows in colonizing habitats worldwide allows us to assess the importance of the variability of Mhc genes in the face of various pathogenic pressures. Isolation and sequencing of Mhc class I and IIB alleles revealed that house sparrows have fewer loci and fewer alleles than great reed warblers. In addition, the Mhc class I genes divided in two distinct lineages with different levels of polymorphism, possibly indicating different functional roles for each gene family. This organization is reminiscent of the chicken B complex and Rfp-Y system. The house sparrow Mhc hence appears to be intermediate between the great reed warbler and the chicken Mhc, both in terms of numbers of alleles and existence of within-class lineages. We specifically amplified one Mhc class I gene family and ran the PCR products on DGGE gels. The individuals screened displayed between one and ten DGGE bands, indicating that this method can be used in future studies to explore the ecological impacts of Mhc diversity. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Immunogenetics
volume
55
issue
12
pages
855 - 865
publisher
Springer
external identifiers
  • wos:000220458900007
  • pmid:14963619
  • scopus:2342504658
ISSN
1432-1211
DOI
10.1007/s00251-004-0648-3
language
English
LU publication?
yes
id
a24f1ae6-e440-4700-a67b-1ff1b01f76e3 (old id 149173)
date added to LUP
2007-06-25 13:02:06
date last changed
2017-08-06 03:31:55
@article{a24f1ae6-e440-4700-a67b-1ff1b01f76e3,
  abstract     = {In order to understand the expression and evolution of host resistance to pathogens, we need to examine the links between genetic variability at the major histocompatibility complex (Mhc), phenotypic expression of the immune response and parasite resistance in natural populations. To do so, we characterized the Mhc class I and IIB genes of house sparrows with the goal of designing a PCR-based genotyping method for the Mhc genes using denaturing gradient gel electrophoresis (DGGE). The incredible success of house sparrows in colonizing habitats worldwide allows us to assess the importance of the variability of Mhc genes in the face of various pathogenic pressures. Isolation and sequencing of Mhc class I and IIB alleles revealed that house sparrows have fewer loci and fewer alleles than great reed warblers. In addition, the Mhc class I genes divided in two distinct lineages with different levels of polymorphism, possibly indicating different functional roles for each gene family. This organization is reminiscent of the chicken B complex and Rfp-Y system. The house sparrow Mhc hence appears to be intermediate between the great reed warbler and the chicken Mhc, both in terms of numbers of alleles and existence of within-class lineages. We specifically amplified one Mhc class I gene family and ran the PCR products on DGGE gels. The individuals screened displayed between one and ten DGGE bands, indicating that this method can be used in future studies to explore the ecological impacts of Mhc diversity.},
  author       = {Bonneaud, C and Sorci, G and Morin, V and Westerdahl, Helena and Zoorob, R and Wittzell, Håkan},
  issn         = {1432-1211},
  language     = {eng},
  number       = {12},
  pages        = {855--865},
  publisher    = {Springer},
  series       = {Immunogenetics},
  title        = {Diversity of Mhc class I and IIB genes in house sparrows (Passer domesticus)},
  url          = {http://dx.doi.org/10.1007/s00251-004-0648-3},
  volume       = {55},
  year         = {2004},
}