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Evaluation of boronate-containing polymer brushes and gels as substrates for carbohydrate-mediated adhesion and cultivation of animal cells.

Ivanov, Alexander LU ; Kumar, Ashok; Nilsang, Suthasinee; Aguilar, Maria-Rosa; Mikhalovska, Lyubov I; Savina, Irina N; Nilsson, Lars LU ; Scheblykin, Ivan LU ; Kuzimenkova, Marina LU and Galaev, Igor LU (2010) In Colloids and Surfaces. B, Biointerfaces 75. p.510-519
Abstract
Boronate-containing thin polyacrylamide gels (B-Gel), polymer brushes (B-Brush) and chemisorbed organosilane layers (B-COSL) were prepared on the surface of glass slides and studied as substrates for carbohydrate-mediated cell adhesion. B-COSL- and B-Brush-modified glass samples exhibited multiple submicron structures densely and irregularly distributed on the glass surface, as found by scanning electron microscopy and atomic force microscopy. B-Gel was ca. 0.1mm thick and contained pores with effective size of 1-2mum in the middle and of 5-20mum on the edges of the gel sample as found by confocal laser scanning microscopy. Evidence for the presence of phenylboronic acid in the samples was given by time-of-flight secondary ion... (More)
Boronate-containing thin polyacrylamide gels (B-Gel), polymer brushes (B-Brush) and chemisorbed organosilane layers (B-COSL) were prepared on the surface of glass slides and studied as substrates for carbohydrate-mediated cell adhesion. B-COSL- and B-Brush-modified glass samples exhibited multiple submicron structures densely and irregularly distributed on the glass surface, as found by scanning electron microscopy and atomic force microscopy. B-Gel was ca. 0.1mm thick and contained pores with effective size of 1-2mum in the middle and of 5-20mum on the edges of the gel sample as found by confocal laser scanning microscopy. Evidence for the presence of phenylboronic acid in the samples was given by time-of-flight secondary ion mass-spectrometry (ToF SIMS), contact angle measurements performed in the presence of fructose, and staining with Alizarin Red S dye capable of formation specific, fluorescent complexes with boronic acids. A comparative study of adhesion and cultivation of animal cells on the above substrates was carried out using murine hybridoma M2139 cell line as a model. M2139 cells adhered to the substrates in the culture medium without glucose or sodium pyruvate at pH 8.0, and then were cultivated in the same medium at pH 7.2 for 4 days. It was found that the substrates of B-Brush type were superior both regarding cell adhesion and viability of the adhered cells, among the substrates studied. MTT assay confirmed proliferation of M2139 cells on B-Brush substrates. Some cell adhesion was also registered in the macropores of B-Gel substrate. The effects of surface microstructure of the boronate-containing polymers on cell adhesion are discussed. Transparent glass substrates grafted with boronate-containing copolymers offer good prospects for cell adhesion studies and development of cell-based assays. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Colloids and Surfaces. B, Biointerfaces
volume
75
pages
510 - 519
publisher
Elsevier
external identifiers
  • wos:000276921900018
  • pmid:19837569
  • scopus:70549106041
ISSN
1873-4367
DOI
10.1016/j.colsurfb.2009.09.028
language
English
LU publication?
yes
id
af49c56e-f71a-474d-8a18-ef2018c4f5b3 (old id 1500252)
date added to LUP
2009-11-06 11:56:27
date last changed
2018-05-29 10:06:18
@article{af49c56e-f71a-474d-8a18-ef2018c4f5b3,
  abstract     = {Boronate-containing thin polyacrylamide gels (B-Gel), polymer brushes (B-Brush) and chemisorbed organosilane layers (B-COSL) were prepared on the surface of glass slides and studied as substrates for carbohydrate-mediated cell adhesion. B-COSL- and B-Brush-modified glass samples exhibited multiple submicron structures densely and irregularly distributed on the glass surface, as found by scanning electron microscopy and atomic force microscopy. B-Gel was ca. 0.1mm thick and contained pores with effective size of 1-2mum in the middle and of 5-20mum on the edges of the gel sample as found by confocal laser scanning microscopy. Evidence for the presence of phenylboronic acid in the samples was given by time-of-flight secondary ion mass-spectrometry (ToF SIMS), contact angle measurements performed in the presence of fructose, and staining with Alizarin Red S dye capable of formation specific, fluorescent complexes with boronic acids. A comparative study of adhesion and cultivation of animal cells on the above substrates was carried out using murine hybridoma M2139 cell line as a model. M2139 cells adhered to the substrates in the culture medium without glucose or sodium pyruvate at pH 8.0, and then were cultivated in the same medium at pH 7.2 for 4 days. It was found that the substrates of B-Brush type were superior both regarding cell adhesion and viability of the adhered cells, among the substrates studied. MTT assay confirmed proliferation of M2139 cells on B-Brush substrates. Some cell adhesion was also registered in the macropores of B-Gel substrate. The effects of surface microstructure of the boronate-containing polymers on cell adhesion are discussed. Transparent glass substrates grafted with boronate-containing copolymers offer good prospects for cell adhesion studies and development of cell-based assays.},
  author       = {Ivanov, Alexander and Kumar, Ashok and Nilsang, Suthasinee and Aguilar, Maria-Rosa and Mikhalovska, Lyubov I and Savina, Irina N and Nilsson, Lars and Scheblykin, Ivan and Kuzimenkova, Marina and Galaev, Igor},
  issn         = {1873-4367},
  language     = {eng},
  pages        = {510--519},
  publisher    = {Elsevier},
  series       = {Colloids and Surfaces. B, Biointerfaces},
  title        = {Evaluation of boronate-containing polymer brushes and gels as substrates for carbohydrate-mediated adhesion and cultivation of animal cells.},
  url          = {http://dx.doi.org/10.1016/j.colsurfb.2009.09.028},
  volume       = {75},
  year         = {2010},
}