Evaluation of boronate-containing polymer brushes and gels as substrates for carbohydrate-mediated adhesion and cultivation of animal cells.
(2010) In Colloids and Surfaces. B, Biointerfaces 75. p.510-519- Abstract
- Boronate-containing thin polyacrylamide gels (B-Gel), polymer brushes (B-Brush) and chemisorbed organosilane layers (B-COSL) were prepared on the surface of glass slides and studied as substrates for carbohydrate-mediated cell adhesion. B-COSL- and B-Brush-modified glass samples exhibited multiple submicron structures densely and irregularly distributed on the glass surface, as found by scanning electron microscopy and atomic force microscopy. B-Gel was ca. 0.1mm thick and contained pores with effective size of 1-2mum in the middle and of 5-20mum on the edges of the gel sample as found by confocal laser scanning microscopy. Evidence for the presence of phenylboronic acid in the samples was given by time-of-flight secondary ion... (More)
- Boronate-containing thin polyacrylamide gels (B-Gel), polymer brushes (B-Brush) and chemisorbed organosilane layers (B-COSL) were prepared on the surface of glass slides and studied as substrates for carbohydrate-mediated cell adhesion. B-COSL- and B-Brush-modified glass samples exhibited multiple submicron structures densely and irregularly distributed on the glass surface, as found by scanning electron microscopy and atomic force microscopy. B-Gel was ca. 0.1mm thick and contained pores with effective size of 1-2mum in the middle and of 5-20mum on the edges of the gel sample as found by confocal laser scanning microscopy. Evidence for the presence of phenylboronic acid in the samples was given by time-of-flight secondary ion mass-spectrometry (ToF SIMS), contact angle measurements performed in the presence of fructose, and staining with Alizarin Red S dye capable of formation specific, fluorescent complexes with boronic acids. A comparative study of adhesion and cultivation of animal cells on the above substrates was carried out using murine hybridoma M2139 cell line as a model. M2139 cells adhered to the substrates in the culture medium without glucose or sodium pyruvate at pH 8.0, and then were cultivated in the same medium at pH 7.2 for 4 days. It was found that the substrates of B-Brush type were superior both regarding cell adhesion and viability of the adhered cells, among the substrates studied. MTT assay confirmed proliferation of M2139 cells on B-Brush substrates. Some cell adhesion was also registered in the macropores of B-Gel substrate. The effects of surface microstructure of the boronate-containing polymers on cell adhesion are discussed. Transparent glass substrates grafted with boronate-containing copolymers offer good prospects for cell adhesion studies and development of cell-based assays. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1500252
- author
- Ivanov, Alexander LU ; Kumar, Ashok ; Nilsang, Suthasinee ; Aguilar, Maria-Rosa ; Mikhalovska, Lyubov I ; Savina, Irina N ; Nilsson, Lars LU ; Scheblykin, Ivan LU ; Kuzimenkova, Marina LU and Galaev, Igor LU
- organization
- publishing date
- 2010
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Colloids and Surfaces. B, Biointerfaces
- volume
- 75
- pages
- 510 - 519
- publisher
- Elsevier
- external identifiers
-
- wos:000276921900018
- pmid:19837569
- scopus:70549106041
- pmid:19837569
- ISSN
- 1873-4367
- DOI
- 10.1016/j.colsurfb.2009.09.028
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Food Technology (011001017), Chemical Physics (S) (011001060), Biotechnology (LTH) (011001037)
- id
- af49c56e-f71a-474d-8a18-ef2018c4f5b3 (old id 1500252)
- date added to LUP
- 2016-04-01 14:35:23
- date last changed
- 2023-09-03 16:31:46
@article{af49c56e-f71a-474d-8a18-ef2018c4f5b3, abstract = {{Boronate-containing thin polyacrylamide gels (B-Gel), polymer brushes (B-Brush) and chemisorbed organosilane layers (B-COSL) were prepared on the surface of glass slides and studied as substrates for carbohydrate-mediated cell adhesion. B-COSL- and B-Brush-modified glass samples exhibited multiple submicron structures densely and irregularly distributed on the glass surface, as found by scanning electron microscopy and atomic force microscopy. B-Gel was ca. 0.1mm thick and contained pores with effective size of 1-2mum in the middle and of 5-20mum on the edges of the gel sample as found by confocal laser scanning microscopy. Evidence for the presence of phenylboronic acid in the samples was given by time-of-flight secondary ion mass-spectrometry (ToF SIMS), contact angle measurements performed in the presence of fructose, and staining with Alizarin Red S dye capable of formation specific, fluorescent complexes with boronic acids. A comparative study of adhesion and cultivation of animal cells on the above substrates was carried out using murine hybridoma M2139 cell line as a model. M2139 cells adhered to the substrates in the culture medium without glucose or sodium pyruvate at pH 8.0, and then were cultivated in the same medium at pH 7.2 for 4 days. It was found that the substrates of B-Brush type were superior both regarding cell adhesion and viability of the adhered cells, among the substrates studied. MTT assay confirmed proliferation of M2139 cells on B-Brush substrates. Some cell adhesion was also registered in the macropores of B-Gel substrate. The effects of surface microstructure of the boronate-containing polymers on cell adhesion are discussed. Transparent glass substrates grafted with boronate-containing copolymers offer good prospects for cell adhesion studies and development of cell-based assays.}}, author = {{Ivanov, Alexander and Kumar, Ashok and Nilsang, Suthasinee and Aguilar, Maria-Rosa and Mikhalovska, Lyubov I and Savina, Irina N and Nilsson, Lars and Scheblykin, Ivan and Kuzimenkova, Marina and Galaev, Igor}}, issn = {{1873-4367}}, language = {{eng}}, pages = {{510--519}}, publisher = {{Elsevier}}, series = {{Colloids and Surfaces. B, Biointerfaces}}, title = {{Evaluation of boronate-containing polymer brushes and gels as substrates for carbohydrate-mediated adhesion and cultivation of animal cells.}}, url = {{http://dx.doi.org/10.1016/j.colsurfb.2009.09.028}}, doi = {{10.1016/j.colsurfb.2009.09.028}}, volume = {{75}}, year = {{2010}}, }