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Amperometric screen-printed biosensor arrays with co-immobilised oxidoreductases and cholinesterases

Solna, R; Dock, Eva LU ; Christenson, Andreas LU ; Winther-Nielsen, M; Carlsson, C; Emnéus, Jenny LU ; Ruzgas, Tautgirdas LU and Skladal, P (2005) In Analytica Chimica Acta 528(1). p.9-19
Abstract
Amperometric screen-printed biosensor arrays for detection of pesticides (organophosphates and carbamates) and phenols have been developed. Cholinesterases (AChE and BChE), tyrosinase (TYR), peroxidases (SBP. soybean and HRP. horseradish) and cellobiose dehydrogenase (CDH) were combined on the same array consisting of one Ag/AgCl reference electrode surrounded by eight radially distributed working electrodes of either carbon or platinum. Mainly cross-linking with glutaraldehyde was employed for enzyme immobilisation. The substrates for the enzymes were acetylthiocholine for cholinesterases (ChEs), cellobiose for CDH and hydrogen peroxide for peroxidases. Hydrogen peroxide was generated in the presence of glucose by co-immobilised glucose... (More)
Amperometric screen-printed biosensor arrays for detection of pesticides (organophosphates and carbamates) and phenols have been developed. Cholinesterases (AChE and BChE), tyrosinase (TYR), peroxidases (SBP. soybean and HRP. horseradish) and cellobiose dehydrogenase (CDH) were combined on the same array consisting of one Ag/AgCl reference electrode surrounded by eight radially distributed working electrodes of either carbon or platinum. Mainly cross-linking with glutaraldehyde was employed for enzyme immobilisation. The substrates for the enzymes were acetylthiocholine for cholinesterases (ChEs), cellobiose for CDH and hydrogen peroxide for peroxidases. Hydrogen peroxide was generated in the presence of glucose by co-immobilised glucose oxidase (GOx). All measurements were performed in an electrochemical steady state system specially constructed for eight channel screen-printed electrode arrays. The achieved relative standard deviation values calculated for different enzyme substrates (10 measurements) were typically below 7% and one assay was completed within less than 10 min. The detection limits for pesticides and phenols were in the nanomolar and micromolar ranges, respectively. The developed biosensor array was evaluated on wastewater samples. To simplify interpretation of results. the measured data were created with multivariate analysis-principal component analysis (PCA). (C) 2004 Elsevier B.V. All rights reserved. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Analytica Chimica Acta
volume
528
issue
1
pages
9 - 19
publisher
Elsevier
external identifiers
  • wos:000226455600002
  • scopus:11844299687
ISSN
1873-4324
DOI
10.1016/j.aca.2004.10.022
language
English
LU publication?
yes
id
f652c8a1-6dec-49d4-a739-7cffc3e4f43e (old id 151154)
date added to LUP
2007-06-27 16:00:42
date last changed
2017-04-02 04:06:23
@article{f652c8a1-6dec-49d4-a739-7cffc3e4f43e,
  abstract     = {Amperometric screen-printed biosensor arrays for detection of pesticides (organophosphates and carbamates) and phenols have been developed. Cholinesterases (AChE and BChE), tyrosinase (TYR), peroxidases (SBP. soybean and HRP. horseradish) and cellobiose dehydrogenase (CDH) were combined on the same array consisting of one Ag/AgCl reference electrode surrounded by eight radially distributed working electrodes of either carbon or platinum. Mainly cross-linking with glutaraldehyde was employed for enzyme immobilisation. The substrates for the enzymes were acetylthiocholine for cholinesterases (ChEs), cellobiose for CDH and hydrogen peroxide for peroxidases. Hydrogen peroxide was generated in the presence of glucose by co-immobilised glucose oxidase (GOx). All measurements were performed in an electrochemical steady state system specially constructed for eight channel screen-printed electrode arrays. The achieved relative standard deviation values calculated for different enzyme substrates (10 measurements) were typically below 7% and one assay was completed within less than 10 min. The detection limits for pesticides and phenols were in the nanomolar and micromolar ranges, respectively. The developed biosensor array was evaluated on wastewater samples. To simplify interpretation of results. the measured data were created with multivariate analysis-principal component analysis (PCA). (C) 2004 Elsevier B.V. All rights reserved.},
  author       = {Solna, R and Dock, Eva and Christenson, Andreas and Winther-Nielsen, M and Carlsson, C and Emnéus, Jenny and Ruzgas, Tautgirdas and Skladal, P},
  issn         = {1873-4324},
  language     = {eng},
  number       = {1},
  pages        = {9--19},
  publisher    = {Elsevier},
  series       = {Analytica Chimica Acta},
  title        = {Amperometric screen-printed biosensor arrays with co-immobilised oxidoreductases and cholinesterases},
  url          = {http://dx.doi.org/10.1016/j.aca.2004.10.022},
  volume       = {528},
  year         = {2005},
}